Unravelling alternative splicing patterns in susceptible and resistant Brassica napus lines in response to Xanthomonas campestris infection
Background: Rapeseed (Brassica napus L.) is an important oil and industrial crop worldwide. Black rot caused by the bacterial pathogen Xanthomonas campestris pv. campestris (Xcc) is an infectious vascular disease that leads to considerable yield losses in rapeseed. Resistance improvement through genetic breeding is an effective and sustainable approach to control black rot disease in B. napus. However, the molecular mechanisms underlying Brassica-Xcc interactions are not yet fully understood, especially regarding the impact of post-transcriptional gene regulation via alternative splicing (AS). Results: In this study, we compared the AS landscapes of a susceptible parental line and two mutagenized B. napus lines with contrasting levels of black rot resistance. Different types of AS events were identified in these B. napus lines at three time points upon Xcc infection, among which intron retention was the most common AS type. A total of 1,932 genes was found to show differential AS patterns between different B. napus lines. Multiple defense-related differential alternative splicing (DAS) hub candidates were pinpointed through an isoform-based co-expression network analysis, including genes involved in pathogen recognition, defense signalling, transcriptional regulation, and oxidation reduction. Conclusion: This study provides new insights into the potential effects of post-transcriptional regulation on immune responses in B. napus towards Xcc attack. These findings could be beneficial for the genetic improvement of B. napus to achieve durable black rot resistance in the future.
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| Format: | Article/Letter to editor biblioteca |
| Language: | English |
| Subjects: | Alternative splicing, Black rot resistance, Brassica napus, RNA-seq, Xanthomonas campestris pv. campestris, |
| Online Access: | https://research.wur.nl/en/publications/unravelling-alternative-splicing-patterns-in-susceptible-and-resi |
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dig-wur-nl-wurpubs-6374002025-01-14 Yang, Li Yang, Lingli Zhao, Chuanji Bai, Zetao Xie, Meili Liu, Jie Cui, Xiaobo Bouwmeester, Klaas Liu, Shengyi Article/Letter to editor BMC Plant Biology 24 (2024) 1 ISSN: 1471-2229 Unravelling alternative splicing patterns in susceptible and resistant Brassica napus lines in response to Xanthomonas campestris infection 2024 Background: Rapeseed (Brassica napus L.) is an important oil and industrial crop worldwide. Black rot caused by the bacterial pathogen Xanthomonas campestris pv. campestris (Xcc) is an infectious vascular disease that leads to considerable yield losses in rapeseed. Resistance improvement through genetic breeding is an effective and sustainable approach to control black rot disease in B. napus. However, the molecular mechanisms underlying Brassica-Xcc interactions are not yet fully understood, especially regarding the impact of post-transcriptional gene regulation via alternative splicing (AS). Results: In this study, we compared the AS landscapes of a susceptible parental line and two mutagenized B. napus lines with contrasting levels of black rot resistance. Different types of AS events were identified in these B. napus lines at three time points upon Xcc infection, among which intron retention was the most common AS type. A total of 1,932 genes was found to show differential AS patterns between different B. napus lines. Multiple defense-related differential alternative splicing (DAS) hub candidates were pinpointed through an isoform-based co-expression network analysis, including genes involved in pathogen recognition, defense signalling, transcriptional regulation, and oxidation reduction. Conclusion: This study provides new insights into the potential effects of post-transcriptional regulation on immune responses in B. napus towards Xcc attack. These findings could be beneficial for the genetic improvement of B. napus to achieve durable black rot resistance in the future. en application/pdf https://research.wur.nl/en/publications/unravelling-alternative-splicing-patterns-in-susceptible-and-resi 10.1186/s12870-024-05728-8 https://edepot.wur.nl/678841 Alternative splicing Black rot resistance Brassica napus RNA-seq Xanthomonas campestris pv. campestris https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/ Wageningen University & Research |
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Alternative splicing Black rot resistance Brassica napus RNA-seq Xanthomonas campestris pv. campestris Alternative splicing Black rot resistance Brassica napus RNA-seq Xanthomonas campestris pv. campestris |
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Alternative splicing Black rot resistance Brassica napus RNA-seq Xanthomonas campestris pv. campestris Alternative splicing Black rot resistance Brassica napus RNA-seq Xanthomonas campestris pv. campestris Yang, Li Yang, Lingli Zhao, Chuanji Bai, Zetao Xie, Meili Liu, Jie Cui, Xiaobo Bouwmeester, Klaas Liu, Shengyi Unravelling alternative splicing patterns in susceptible and resistant Brassica napus lines in response to Xanthomonas campestris infection |
| description |
Background: Rapeseed (Brassica napus L.) is an important oil and industrial crop worldwide. Black rot caused by the bacterial pathogen Xanthomonas campestris pv. campestris (Xcc) is an infectious vascular disease that leads to considerable yield losses in rapeseed. Resistance improvement through genetic breeding is an effective and sustainable approach to control black rot disease in B. napus. However, the molecular mechanisms underlying Brassica-Xcc interactions are not yet fully understood, especially regarding the impact of post-transcriptional gene regulation via alternative splicing (AS). Results: In this study, we compared the AS landscapes of a susceptible parental line and two mutagenized B. napus lines with contrasting levels of black rot resistance. Different types of AS events were identified in these B. napus lines at three time points upon Xcc infection, among which intron retention was the most common AS type. A total of 1,932 genes was found to show differential AS patterns between different B. napus lines. Multiple defense-related differential alternative splicing (DAS) hub candidates were pinpointed through an isoform-based co-expression network analysis, including genes involved in pathogen recognition, defense signalling, transcriptional regulation, and oxidation reduction. Conclusion: This study provides new insights into the potential effects of post-transcriptional regulation on immune responses in B. napus towards Xcc attack. These findings could be beneficial for the genetic improvement of B. napus to achieve durable black rot resistance in the future. |
| format |
Article/Letter to editor |
| topic_facet |
Alternative splicing Black rot resistance Brassica napus RNA-seq Xanthomonas campestris pv. campestris |
| author |
Yang, Li Yang, Lingli Zhao, Chuanji Bai, Zetao Xie, Meili Liu, Jie Cui, Xiaobo Bouwmeester, Klaas Liu, Shengyi |
| author_facet |
Yang, Li Yang, Lingli Zhao, Chuanji Bai, Zetao Xie, Meili Liu, Jie Cui, Xiaobo Bouwmeester, Klaas Liu, Shengyi |
| author_sort |
Yang, Li |
| title |
Unravelling alternative splicing patterns in susceptible and resistant Brassica napus lines in response to Xanthomonas campestris infection |
| title_short |
Unravelling alternative splicing patterns in susceptible and resistant Brassica napus lines in response to Xanthomonas campestris infection |
| title_full |
Unravelling alternative splicing patterns in susceptible and resistant Brassica napus lines in response to Xanthomonas campestris infection |
| title_fullStr |
Unravelling alternative splicing patterns in susceptible and resistant Brassica napus lines in response to Xanthomonas campestris infection |
| title_full_unstemmed |
Unravelling alternative splicing patterns in susceptible and resistant Brassica napus lines in response to Xanthomonas campestris infection |
| title_sort |
unravelling alternative splicing patterns in susceptible and resistant brassica napus lines in response to xanthomonas campestris infection |
| url |
https://research.wur.nl/en/publications/unravelling-alternative-splicing-patterns-in-susceptible-and-resi |
| work_keys_str_mv |
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1822262543913058304 |