Tongue-on-a-Chip: Parallel Recording of Sweet and Bitter Receptor Responses to Sequential Injections of Pure and Mixed Sweeteners
A microfluidic tongue-on-a-chip platform has been evaluated relative to the known sensory properties of various sweeteners. Analogous metrics of typical sensory features reported by human panels such as sweet taste thresholds, onset, and lingering, as well as bitter off-flavor and blocking interactions were deduced from the taste receptor activation curves and then compared. To this end, a flow cell containing a receptor cell array bearing the sweet and six bitter taste receptors was transiently exposed to pure and mixed sweetener samples. The sample concentration gradient across time was separately characterized by the injection of fluorescein dye. Subsequently, cellular calcium responses to different doses of advantame, aspartame, saccharine, and sucrose were overlaid with the concentration gradient. Parameters describing the response kinetics compared to the gradient were quantified. Advantame at 15 μM recorded a significantly faster sweetness onset of 5 ± 2 s and a longer lingering time of 39 s relative to sucrose at 100 mM with an onset of 13 ± 2 s and a lingering time of 6 s. Saccharine was shown to activate the bitter receptors TAS2R8, TAS2R31, and TAS2R43, confirming its known off-flavor, whereas addition of cyclamate reduced or blocked this saccharine bitter response. The potential of using this tongue-on-a-chip to bridge the gap with in vitro assays and taste panels is discussed.
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| Format: | Article/Letter to editor biblioteca |
| Language: | English |
| Subjects: | EC50, GPCR, antagonism, bitter blocking, lingering, microfluidics, off-taste, onset, taste threshold, |
| Online Access: | https://research.wur.nl/en/publications/tongue-on-a-chip-parallel-recording-of-sweet-and-bitter-receptor- |
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dig-wur-nl-wurpubs-6330472025-01-14 Roelse, Margriet Krasteva, Nadejda Pawlizak, Steve Mai, Michaela K. Jongsma, Maarten A. Article/Letter to editor Journal of Agricultural and Food Chemistry 72 (2024) 28 ISSN: 0021-8561 Tongue-on-a-Chip: Parallel Recording of Sweet and Bitter Receptor Responses to Sequential Injections of Pure and Mixed Sweeteners 2024 A microfluidic tongue-on-a-chip platform has been evaluated relative to the known sensory properties of various sweeteners. Analogous metrics of typical sensory features reported by human panels such as sweet taste thresholds, onset, and lingering, as well as bitter off-flavor and blocking interactions were deduced from the taste receptor activation curves and then compared. To this end, a flow cell containing a receptor cell array bearing the sweet and six bitter taste receptors was transiently exposed to pure and mixed sweetener samples. The sample concentration gradient across time was separately characterized by the injection of fluorescein dye. Subsequently, cellular calcium responses to different doses of advantame, aspartame, saccharine, and sucrose were overlaid with the concentration gradient. Parameters describing the response kinetics compared to the gradient were quantified. Advantame at 15 μM recorded a significantly faster sweetness onset of 5 ± 2 s and a longer lingering time of 39 s relative to sucrose at 100 mM with an onset of 13 ± 2 s and a lingering time of 6 s. Saccharine was shown to activate the bitter receptors TAS2R8, TAS2R31, and TAS2R43, confirming its known off-flavor, whereas addition of cyclamate reduced or blocked this saccharine bitter response. The potential of using this tongue-on-a-chip to bridge the gap with in vitro assays and taste panels is discussed. en application/pdf https://research.wur.nl/en/publications/tongue-on-a-chip-parallel-recording-of-sweet-and-bitter-receptor- 10.1021/acs.jafc.4c00815 https://edepot.wur.nl/670936 EC50 GPCR antagonism bitter blocking lingering microfluidics off-taste onset taste threshold https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/ Wageningen University & Research |
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EC50 GPCR antagonism bitter blocking lingering microfluidics off-taste onset taste threshold EC50 GPCR antagonism bitter blocking lingering microfluidics off-taste onset taste threshold |
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EC50 GPCR antagonism bitter blocking lingering microfluidics off-taste onset taste threshold EC50 GPCR antagonism bitter blocking lingering microfluidics off-taste onset taste threshold Roelse, Margriet Krasteva, Nadejda Pawlizak, Steve Mai, Michaela K. Jongsma, Maarten A. Tongue-on-a-Chip: Parallel Recording of Sweet and Bitter Receptor Responses to Sequential Injections of Pure and Mixed Sweeteners |
| description |
A microfluidic tongue-on-a-chip platform has been evaluated relative to the known sensory properties of various sweeteners. Analogous metrics of typical sensory features reported by human panels such as sweet taste thresholds, onset, and lingering, as well as bitter off-flavor and blocking interactions were deduced from the taste receptor activation curves and then compared. To this end, a flow cell containing a receptor cell array bearing the sweet and six bitter taste receptors was transiently exposed to pure and mixed sweetener samples. The sample concentration gradient across time was separately characterized by the injection of fluorescein dye. Subsequently, cellular calcium responses to different doses of advantame, aspartame, saccharine, and sucrose were overlaid with the concentration gradient. Parameters describing the response kinetics compared to the gradient were quantified. Advantame at 15 μM recorded a significantly faster sweetness onset of 5 ± 2 s and a longer lingering time of 39 s relative to sucrose at 100 mM with an onset of 13 ± 2 s and a lingering time of 6 s. Saccharine was shown to activate the bitter receptors TAS2R8, TAS2R31, and TAS2R43, confirming its known off-flavor, whereas addition of cyclamate reduced or blocked this saccharine bitter response. The potential of using this tongue-on-a-chip to bridge the gap with in vitro assays and taste panels is discussed. |
| format |
Article/Letter to editor |
| topic_facet |
EC50 GPCR antagonism bitter blocking lingering microfluidics off-taste onset taste threshold |
| author |
Roelse, Margriet Krasteva, Nadejda Pawlizak, Steve Mai, Michaela K. Jongsma, Maarten A. |
| author_facet |
Roelse, Margriet Krasteva, Nadejda Pawlizak, Steve Mai, Michaela K. Jongsma, Maarten A. |
| author_sort |
Roelse, Margriet |
| title |
Tongue-on-a-Chip: Parallel Recording of Sweet and Bitter Receptor Responses to Sequential Injections of Pure and Mixed Sweeteners |
| title_short |
Tongue-on-a-Chip: Parallel Recording of Sweet and Bitter Receptor Responses to Sequential Injections of Pure and Mixed Sweeteners |
| title_full |
Tongue-on-a-Chip: Parallel Recording of Sweet and Bitter Receptor Responses to Sequential Injections of Pure and Mixed Sweeteners |
| title_fullStr |
Tongue-on-a-Chip: Parallel Recording of Sweet and Bitter Receptor Responses to Sequential Injections of Pure and Mixed Sweeteners |
| title_full_unstemmed |
Tongue-on-a-Chip: Parallel Recording of Sweet and Bitter Receptor Responses to Sequential Injections of Pure and Mixed Sweeteners |
| title_sort |
tongue-on-a-chip: parallel recording of sweet and bitter receptor responses to sequential injections of pure and mixed sweeteners |
| url |
https://research.wur.nl/en/publications/tongue-on-a-chip-parallel-recording-of-sweet-and-bitter-receptor- |
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