Hypoglycaemia induces a sustained pro-inflammatory response in people with type 1 diabetes and healthy controls
Aim: To determine the duration and the extension of the pro-inflammatory response to hypoglycaemia both in people with type 1 diabetes and healthy controls. Materials and Methods: Adults with type 1 diabetes (n = 47) and matched controls (n = 16) underwent a hyperinsulinaemic-euglycaemic hypoglycaemic (2.8 ± 0.1 mmoL/L [49.9 ± 2.3 mg/dL]) glucose clamp. During euglycaemia, hypoglycaemia, and 1, 3 and 7 days later, blood was drawn to determine immune cell phenotype, monocyte function and circulating inflammatory markers. Results: Hypoglycaemia increased lymphocyte and monocyte counts, which remained elevated for 1 week. The proportion of CD16+ monocytes increased and the proportion of CD14+ monocytes decreased. During hypoglycaemia, monocytes released more tumour necrosis factor-α and interleukin-1β, and less interleukin-10, after ex vivo stimulation. Hypoglycaemia increased the levels of 19 circulating inflammatory proteins, including high sensitive C-reactive protein, most of which remained elevated for 1 week. The epinephrine peak in response to hypoglycaemia was positively correlated with immune cell number and phenotype, but not with the proteomic response. Conclusions: Overall, despite differences in prior exposure to hypoglycaemia, the pattern of the inflammatory responses to hypoglycaemia did not differ between people with type 1 diabetes and healthy controls. In conclusion, hypoglycaemia induces a range of pro-inflammatory responses that are sustained for at least 1 week in people with type 1 diabetes and healthy controls.
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| Language: | English |
| Subjects: | C-reactive protein, clamp, counterregulatory hormones, diabetes, hypoglycaemia, inflammation, white blood cells, |
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dig-wur-nl-wurpubs-6178242024-12-04 Verhulst, Clementine E.M. van Heck, Julia I.P. Fabricius, Therese W. Stienstra, Rinke Teerenstra, Steven McCrimmon, Rory J. Tack, Cees J. Pedersen-Bjergaard, Ulrik de Galan, Bastiaan E. Article/Letter to editor Diabetes, Obesity and Metabolism 25 (2023) 11 ISSN: 1462-8902 Hypoglycaemia induces a sustained pro-inflammatory response in people with type 1 diabetes and healthy controls 2023 Aim: To determine the duration and the extension of the pro-inflammatory response to hypoglycaemia both in people with type 1 diabetes and healthy controls. Materials and Methods: Adults with type 1 diabetes (n = 47) and matched controls (n = 16) underwent a hyperinsulinaemic-euglycaemic hypoglycaemic (2.8 ± 0.1 mmoL/L [49.9 ± 2.3 mg/dL]) glucose clamp. During euglycaemia, hypoglycaemia, and 1, 3 and 7 days later, blood was drawn to determine immune cell phenotype, monocyte function and circulating inflammatory markers. Results: Hypoglycaemia increased lymphocyte and monocyte counts, which remained elevated for 1 week. The proportion of CD16+ monocytes increased and the proportion of CD14+ monocytes decreased. During hypoglycaemia, monocytes released more tumour necrosis factor-α and interleukin-1β, and less interleukin-10, after ex vivo stimulation. Hypoglycaemia increased the levels of 19 circulating inflammatory proteins, including high sensitive C-reactive protein, most of which remained elevated for 1 week. The epinephrine peak in response to hypoglycaemia was positively correlated with immune cell number and phenotype, but not with the proteomic response. Conclusions: Overall, despite differences in prior exposure to hypoglycaemia, the pattern of the inflammatory responses to hypoglycaemia did not differ between people with type 1 diabetes and healthy controls. In conclusion, hypoglycaemia induces a range of pro-inflammatory responses that are sustained for at least 1 week in people with type 1 diabetes and healthy controls. en application/pdf https://research.wur.nl/en/publications/hypoglycaemia-induces-a-sustained-pro-inflammatory-response-in-pe 10.1111/dom.15205 https://edepot.wur.nl/636706 C-reactive protein clamp counterregulatory hormones diabetes hypoglycaemia inflammation white blood cells https://creativecommons.org/licenses/by-nc/4.0/ https://creativecommons.org/licenses/by-nc/4.0/ Wageningen University & Research |
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C-reactive protein clamp counterregulatory hormones diabetes hypoglycaemia inflammation white blood cells C-reactive protein clamp counterregulatory hormones diabetes hypoglycaemia inflammation white blood cells |
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C-reactive protein clamp counterregulatory hormones diabetes hypoglycaemia inflammation white blood cells C-reactive protein clamp counterregulatory hormones diabetes hypoglycaemia inflammation white blood cells Verhulst, Clementine E.M. van Heck, Julia I.P. Fabricius, Therese W. Stienstra, Rinke Teerenstra, Steven McCrimmon, Rory J. Tack, Cees J. Pedersen-Bjergaard, Ulrik de Galan, Bastiaan E. Hypoglycaemia induces a sustained pro-inflammatory response in people with type 1 diabetes and healthy controls |
| description |
Aim: To determine the duration and the extension of the pro-inflammatory response to hypoglycaemia both in people with type 1 diabetes and healthy controls. Materials and Methods: Adults with type 1 diabetes (n = 47) and matched controls (n = 16) underwent a hyperinsulinaemic-euglycaemic hypoglycaemic (2.8 ± 0.1 mmoL/L [49.9 ± 2.3 mg/dL]) glucose clamp. During euglycaemia, hypoglycaemia, and 1, 3 and 7 days later, blood was drawn to determine immune cell phenotype, monocyte function and circulating inflammatory markers. Results: Hypoglycaemia increased lymphocyte and monocyte counts, which remained elevated for 1 week. The proportion of CD16+ monocytes increased and the proportion of CD14+ monocytes decreased. During hypoglycaemia, monocytes released more tumour necrosis factor-α and interleukin-1β, and less interleukin-10, after ex vivo stimulation. Hypoglycaemia increased the levels of 19 circulating inflammatory proteins, including high sensitive C-reactive protein, most of which remained elevated for 1 week. The epinephrine peak in response to hypoglycaemia was positively correlated with immune cell number and phenotype, but not with the proteomic response. Conclusions: Overall, despite differences in prior exposure to hypoglycaemia, the pattern of the inflammatory responses to hypoglycaemia did not differ between people with type 1 diabetes and healthy controls. In conclusion, hypoglycaemia induces a range of pro-inflammatory responses that are sustained for at least 1 week in people with type 1 diabetes and healthy controls. |
| format |
Article/Letter to editor |
| topic_facet |
C-reactive protein clamp counterregulatory hormones diabetes hypoglycaemia inflammation white blood cells |
| author |
Verhulst, Clementine E.M. van Heck, Julia I.P. Fabricius, Therese W. Stienstra, Rinke Teerenstra, Steven McCrimmon, Rory J. Tack, Cees J. Pedersen-Bjergaard, Ulrik de Galan, Bastiaan E. |
| author_facet |
Verhulst, Clementine E.M. van Heck, Julia I.P. Fabricius, Therese W. Stienstra, Rinke Teerenstra, Steven McCrimmon, Rory J. Tack, Cees J. Pedersen-Bjergaard, Ulrik de Galan, Bastiaan E. |
| author_sort |
Verhulst, Clementine E.M. |
| title |
Hypoglycaemia induces a sustained pro-inflammatory response in people with type 1 diabetes and healthy controls |
| title_short |
Hypoglycaemia induces a sustained pro-inflammatory response in people with type 1 diabetes and healthy controls |
| title_full |
Hypoglycaemia induces a sustained pro-inflammatory response in people with type 1 diabetes and healthy controls |
| title_fullStr |
Hypoglycaemia induces a sustained pro-inflammatory response in people with type 1 diabetes and healthy controls |
| title_full_unstemmed |
Hypoglycaemia induces a sustained pro-inflammatory response in people with type 1 diabetes and healthy controls |
| title_sort |
hypoglycaemia induces a sustained pro-inflammatory response in people with type 1 diabetes and healthy controls |
| url |
https://research.wur.nl/en/publications/hypoglycaemia-induces-a-sustained-pro-inflammatory-response-in-pe |
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