Potato protein tyrosine phosphatase StPTP1a is activated by StMKK1 to negatively regulate plant immunity
Phytophthora infestans causes severe losses in potato production. The MAPK kinase StMKK1 was previously found to negatively regulate potato immunity to P. infestans. Our results showed that StMKK1 interacts with a protein tyrosine phosphatase, referred to as StPTP1a, and StMKK1 directly phosphorylates StPTP1a at residues Ser-99, Tyr-223 and Thr-290. StPTP1a is a functional phosphatase and the phosphorylation of StPTP1a at these three residues enhances its stability and catalytic activity. StPTP1a negatively regulates potato immunity and represses SA-related gene expression. Furthermore, StPTP1a interacts with, and dephosphorylates, the StMKK1 downstream signalling targets StMPK4 and −7 at their Tyr-203 residue resulting in the repression of salicylic acid (SA)-related immunity. Silencing of NbPTP1a + NbMPK4 or NbPTP1a + NbMPK7 abolished the plant immunity to P. infestans caused by NbPTP1a silencing, indicating that PTP1a functions upstream of NbMPK4 and NbMPK7. StMKK1 requires StPTP1a to negatively regulate SA-related immunity and StPTP1a is phosphorylated and stabilized during immune activation to promote the de-phosphorylation of StMPK4 and −7. Our results reveal that potato StMKK1 activates and stabilizes the tyrosine phosphatase StPTP1a that in its turn de-phosphorylates StMPK4 and −7, thereby repressing plant SA-related immunity.
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Format: | Article/Letter to editor biblioteca |
Language: | English |
Subjects: | MAPK, Phytophthora infestans, oomycete, potato, resistance, |
Online Access: | https://research.wur.nl/en/publications/potato-protein-tyrosine-phosphatase-stptp1a-is-activated-by-stmkk |
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dig-wur-nl-wurpubs-6104412024-10-02 Li, Fangfang Chen, Xiaokang Yang, Ruixin Zhang, Kun Shan, Weixing Joosten, Matthieu H.A.J. Du, Yu Article/Letter to editor Plant Biotechnology Journal 21 (2023) 3 ISSN: 1467-7644 Potato protein tyrosine phosphatase StPTP1a is activated by StMKK1 to negatively regulate plant immunity 2023 Phytophthora infestans causes severe losses in potato production. The MAPK kinase StMKK1 was previously found to negatively regulate potato immunity to P. infestans. Our results showed that StMKK1 interacts with a protein tyrosine phosphatase, referred to as StPTP1a, and StMKK1 directly phosphorylates StPTP1a at residues Ser-99, Tyr-223 and Thr-290. StPTP1a is a functional phosphatase and the phosphorylation of StPTP1a at these three residues enhances its stability and catalytic activity. StPTP1a negatively regulates potato immunity and represses SA-related gene expression. Furthermore, StPTP1a interacts with, and dephosphorylates, the StMKK1 downstream signalling targets StMPK4 and −7 at their Tyr-203 residue resulting in the repression of salicylic acid (SA)-related immunity. Silencing of NbPTP1a + NbMPK4 or NbPTP1a + NbMPK7 abolished the plant immunity to P. infestans caused by NbPTP1a silencing, indicating that PTP1a functions upstream of NbMPK4 and NbMPK7. StMKK1 requires StPTP1a to negatively regulate SA-related immunity and StPTP1a is phosphorylated and stabilized during immune activation to promote the de-phosphorylation of StMPK4 and −7. Our results reveal that potato StMKK1 activates and stabilizes the tyrosine phosphatase StPTP1a that in its turn de-phosphorylates StMPK4 and −7, thereby repressing plant SA-related immunity. en application/pdf https://research.wur.nl/en/publications/potato-protein-tyrosine-phosphatase-stptp1a-is-activated-by-stmkk 10.1111/pbi.13979 https://edepot.wur.nl/587180 MAPK Phytophthora infestans oomycete potato resistance https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by/4.0/ Wageningen University & Research |
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MAPK Phytophthora infestans oomycete potato resistance MAPK Phytophthora infestans oomycete potato resistance |
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MAPK Phytophthora infestans oomycete potato resistance MAPK Phytophthora infestans oomycete potato resistance Li, Fangfang Chen, Xiaokang Yang, Ruixin Zhang, Kun Shan, Weixing Joosten, Matthieu H.A.J. Du, Yu Potato protein tyrosine phosphatase StPTP1a is activated by StMKK1 to negatively regulate plant immunity |
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Phytophthora infestans causes severe losses in potato production. The MAPK kinase StMKK1 was previously found to negatively regulate potato immunity to P. infestans. Our results showed that StMKK1 interacts with a protein tyrosine phosphatase, referred to as StPTP1a, and StMKK1 directly phosphorylates StPTP1a at residues Ser-99, Tyr-223 and Thr-290. StPTP1a is a functional phosphatase and the phosphorylation of StPTP1a at these three residues enhances its stability and catalytic activity. StPTP1a negatively regulates potato immunity and represses SA-related gene expression. Furthermore, StPTP1a interacts with, and dephosphorylates, the StMKK1 downstream signalling targets StMPK4 and −7 at their Tyr-203 residue resulting in the repression of salicylic acid (SA)-related immunity. Silencing of NbPTP1a + NbMPK4 or NbPTP1a + NbMPK7 abolished the plant immunity to P. infestans caused by NbPTP1a silencing, indicating that PTP1a functions upstream of NbMPK4 and NbMPK7. StMKK1 requires StPTP1a to negatively regulate SA-related immunity and StPTP1a is phosphorylated and stabilized during immune activation to promote the de-phosphorylation of StMPK4 and −7. Our results reveal that potato StMKK1 activates and stabilizes the tyrosine phosphatase StPTP1a that in its turn de-phosphorylates StMPK4 and −7, thereby repressing plant SA-related immunity. |
format |
Article/Letter to editor |
topic_facet |
MAPK Phytophthora infestans oomycete potato resistance |
author |
Li, Fangfang Chen, Xiaokang Yang, Ruixin Zhang, Kun Shan, Weixing Joosten, Matthieu H.A.J. Du, Yu |
author_facet |
Li, Fangfang Chen, Xiaokang Yang, Ruixin Zhang, Kun Shan, Weixing Joosten, Matthieu H.A.J. Du, Yu |
author_sort |
Li, Fangfang |
title |
Potato protein tyrosine phosphatase StPTP1a is activated by StMKK1 to negatively regulate plant immunity |
title_short |
Potato protein tyrosine phosphatase StPTP1a is activated by StMKK1 to negatively regulate plant immunity |
title_full |
Potato protein tyrosine phosphatase StPTP1a is activated by StMKK1 to negatively regulate plant immunity |
title_fullStr |
Potato protein tyrosine phosphatase StPTP1a is activated by StMKK1 to negatively regulate plant immunity |
title_full_unstemmed |
Potato protein tyrosine phosphatase StPTP1a is activated by StMKK1 to negatively regulate plant immunity |
title_sort |
potato protein tyrosine phosphatase stptp1a is activated by stmkk1 to negatively regulate plant immunity |
url |
https://research.wur.nl/en/publications/potato-protein-tyrosine-phosphatase-stptp1a-is-activated-by-stmkk |
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