GH10 and GH11 endoxylanases in Penicillium subrubescens : Comparative characterization and synergy with GH51, GH54, GH62 α-L-arabinofuranosidases from the same fungus
Penicillium subrubescens has an expanded set of genes encoding putative endoxylanases (PsXLNs) compared to most other Penicillia and other fungi. In this study, all GH10 and GH11 PsXLNs were produced heterologously in Pichia pastoris and characterized. They were active towards beech wood xylan (BWX) and wheat flour arabinoxylan (WAX), and showed stability over a wide pH range. Additionally, PsXLNs released distinct oligosaccharides from WAX, and showed significant cooperative action with P. subrubescens α-L-arabinofuranosidases (PsABFs) from GH51 or GH54 for WAX degradation, giving insight into a more diverse XLN and ABF system for the efficient degradation of complex hemicelluloses. Homology modeling analysis pointed out differences in the catalytic center of PsXLNs, which are discussed in view of the different modes of action observed. These findings facilitate understanding of structural requirements for substrate recognition to contribute to recombinant XLN engineering for biotechnological applications.
Main Authors: | Li, Xinxin, Kouzounis, Dimitrios, Kabel, Mirjam A., de Vries, Ronald P. |
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Format: | Article/Letter to editor biblioteca |
Language: | English |
Subjects: | Functional diversity, Gene expansion, Penicillium subrubescens, Structural diversity, Xylan degradation, |
Online Access: | https://research.wur.nl/en/publications/gh10-and-gh11-endoxylanases-in-penicillium-subrubescens-comparati |
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