The genome of the haptophyte Diacronema lutheri (Pavlova lutheri, Pavlovales)

Haptophytes are biogeochemically and industrially important protists, but we know little about their genomic diversity. Here we sequenced the nuclear genome of Diacronema lutheri (Pavlova lutheri, Pavlovales) using third generation PacBio long-read sequencing coupled with Illumina sequencing. The genome was assembled with CANU and subsequently polished using both PacBio and Illumina reads to high consensus accuracy. Genome annotation was performed using BRAKER2 and three sets of RNA-seq evidence. The dataset presented here comprises two files comprising (1) the full genome sequence and (2) the annotated protein sequences, each in .fasta format.The genome assembly size is 43.5 Mb and is characterized by a high GC content. Due to the use of long sequencing reads, the genome is highly contiguous and contained in 103 contigs. A total of 14,446 protein-coding genes were annotated, and further details of the assembly and annotation process are available in the publication.

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Bibliographic Details
Main Authors: Hulatt, Chris J., Wijffels, René H., Posewitz, Matthew C.
Format: Dataset biblioteca
Published: Nord University
Subjects:PacBio sequencing, genome assembly, genome evolution, haptophytes, natural sciences, protist,
Online Access:https://research.wur.nl/en/datasets/the-genome-of-the-haptophyte-diacronema-lutheri-pavlova-lutheri-p
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spelling dig-wur-nl-wurpubs-5978392024-09-20 Hulatt, Chris J. Wijffels, René H. Posewitz, Matthew C. Dataset The genome of the haptophyte Diacronema lutheri (Pavlova lutheri, Pavlovales) 2021 Haptophytes are biogeochemically and industrially important protists, but we know little about their genomic diversity. Here we sequenced the nuclear genome of Diacronema lutheri (Pavlova lutheri, Pavlovales) using third generation PacBio long-read sequencing coupled with Illumina sequencing. The genome was assembled with CANU and subsequently polished using both PacBio and Illumina reads to high consensus accuracy. Genome annotation was performed using BRAKER2 and three sets of RNA-seq evidence. The dataset presented here comprises two files comprising (1) the full genome sequence and (2) the annotated protein sequences, each in .fasta format.The genome assembly size is 43.5 Mb and is characterized by a high GC content. Due to the use of long sequencing reads, the genome is highly contiguous and contained in 103 contigs. A total of 14,446 protein-coding genes were annotated, and further details of the assembly and annotation process are available in the publication. Haptophytes are biogeochemically and industrially important protists, but we know little about their genomic diversity. Here we sequenced the nuclear genome of Diacronema lutheri (Pavlova lutheri, Pavlovales) using third generation PacBio long-read sequencing coupled with Illumina sequencing. The genome was assembled with CANU and subsequently polished using both PacBio and Illumina reads to high consensus accuracy. Genome annotation was performed using BRAKER2 and three sets of RNA-seq evidence. The dataset presented here comprises two files comprising (1) the full genome sequence and (2) the annotated protein sequences, each in .fasta format.The genome assembly size is 43.5 Mb and is characterized by a high GC content. Due to the use of long sequencing reads, the genome is highly contiguous and contained in 103 contigs. A total of 14,446 protein-coding genes were annotated, and further details of the assembly and annotation process are available in the publication. Nord University text/html https://research.wur.nl/en/datasets/the-genome-of-the-haptophyte-diacronema-lutheri-pavlova-lutheri-p 10.5061/dryad.5qfttdz55 https://edepot.wur.nl/570621 PacBio sequencing genome assembly genome evolution haptophytes natural sciences protist Wageningen University & Research
institution WUR NL
collection DSpace
country Países bajos
countrycode NL
component Bibliográfico
access En linea
databasecode dig-wur-nl
tag biblioteca
region Europa del Oeste
libraryname WUR Library Netherlands
topic PacBio sequencing
genome assembly
genome evolution
haptophytes
natural sciences
protist
PacBio sequencing
genome assembly
genome evolution
haptophytes
natural sciences
protist
spellingShingle PacBio sequencing
genome assembly
genome evolution
haptophytes
natural sciences
protist
PacBio sequencing
genome assembly
genome evolution
haptophytes
natural sciences
protist
Hulatt, Chris J.
Wijffels, René H.
Posewitz, Matthew C.
The genome of the haptophyte Diacronema lutheri (Pavlova lutheri, Pavlovales)
description Haptophytes are biogeochemically and industrially important protists, but we know little about their genomic diversity. Here we sequenced the nuclear genome of Diacronema lutheri (Pavlova lutheri, Pavlovales) using third generation PacBio long-read sequencing coupled with Illumina sequencing. The genome was assembled with CANU and subsequently polished using both PacBio and Illumina reads to high consensus accuracy. Genome annotation was performed using BRAKER2 and three sets of RNA-seq evidence. The dataset presented here comprises two files comprising (1) the full genome sequence and (2) the annotated protein sequences, each in .fasta format.The genome assembly size is 43.5 Mb and is characterized by a high GC content. Due to the use of long sequencing reads, the genome is highly contiguous and contained in 103 contigs. A total of 14,446 protein-coding genes were annotated, and further details of the assembly and annotation process are available in the publication.
format Dataset
topic_facet PacBio sequencing
genome assembly
genome evolution
haptophytes
natural sciences
protist
author Hulatt, Chris J.
Wijffels, René H.
Posewitz, Matthew C.
author_facet Hulatt, Chris J.
Wijffels, René H.
Posewitz, Matthew C.
author_sort Hulatt, Chris J.
title The genome of the haptophyte Diacronema lutheri (Pavlova lutheri, Pavlovales)
title_short The genome of the haptophyte Diacronema lutheri (Pavlova lutheri, Pavlovales)
title_full The genome of the haptophyte Diacronema lutheri (Pavlova lutheri, Pavlovales)
title_fullStr The genome of the haptophyte Diacronema lutheri (Pavlova lutheri, Pavlovales)
title_full_unstemmed The genome of the haptophyte Diacronema lutheri (Pavlova lutheri, Pavlovales)
title_sort genome of the haptophyte diacronema lutheri (pavlova lutheri, pavlovales)
publisher Nord University
url https://research.wur.nl/en/datasets/the-genome-of-the-haptophyte-diacronema-lutheri-pavlova-lutheri-p
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