In vivo formation of arabinoxylo-oligosaccharides by dietary endo-xylanase alters arabinoxylan utilization in broilers

Previously, arabinoxylan (AX) depolymerization by dietary endo-xylanase was observed in the broiler ileum, but released arabinoxylo-oligosaccharides (AXOS) were not characterized in detail. This study aimed at extracting and identifying AXOS released in vivo in broilers, in order to delineate the influence of endo-xylanase on AX utilization. Hereto, digesta from the gizzard, ileum, ceca and excreta of broilers fed a wheat-soybean diet without (Con) or with endo-xylanase supplementation (Enz) were assessed. Soluble AX content in the ileum was higher for Enz diet (26.9%) than for Con diet (18.8%), indicating a different type and amount of AX entering the ceca. Removal of maltodextrins and fructans enabled monitoring of AX depolymerization to AXOS (Enz diet) using HPSEC-RI and HPAEC-PAD. A recently developed HILIC-MSn methodology allowed AXOS (DP 4–10) identification in ileal digesta and excreta. Xylanase-induced AXOS formation coincided with decreased total tract AX recovery, which indicated improved AX hindgut utilization.

Bibliographic Details

Main Authors:	Kouzounis, Dimitrios, Jonathan, Melliana C., Soares, Natalia, Kabel, Mirjam A., Schols, Henk A.
Format:	Article/Letter to editor biblioteca
Language:	English
Subjects:	Arabinoxylan, Arabinoxylo-oligosaccharides, Broiler, In vivo, endo-xylanase,
Online Access:	https://research.wur.nl/en/publications/in-vivo-formation-of-arabinoxylo-oligosaccharides-by-dietary-endo
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