Galacto-oligosaccharides as an anti-bacterial and anti-invasive agent in lung infections
Emerging antimicrobial resistance in infections asks for novel intervention strategies. Galacto-oligosaccharides (GOS) might be attractive alternatives to antibiotics due to their anti-inflammatory and anti-adhesive properties. Mannheimia haemolytica is one of the major Pasteurellaceae associated with bovine lung infections. Using M. haemolytica, we demonstrated that GOS have the capacity to reduce bacterial viability and can be used as adjuvant to improve antibiotic efficacy. Using M. haemolytica-treated primary bronchial epithelial cells (PBECs) of calves, we identified the anti-adhesive and anti-invasive activities of GOS. The observed inhibition of cytokine/chemokine release and the prevention of airway epithelial barrier dysfunction in M. haemolytica-treated PBECs by GOS might be related to the downregulation of “toll-like receptor 4/nuclear factor-κB” pathway and the anti-invasive and anti-adhesive properties of GOS. Particularly, GOS lowered lipopolysaccharides- but not flagellin-induced cytokine/chemokine release in calf and human airway epithelial cells. Finally, we performed in vivo experiments in calves and demonstrated for the first time that intranasal application of GOS can relieve lung infections/inflammation and lower M. haemolytica positivity in the lungs without affecting clinical performance. These findings not only shed light on the anti-inflammatory mechanisms of GOS during lung infections, but GOS might also be a promising anti-bacterial agent for preventing (lung) infections.
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| Format: | Article/Letter to editor biblioteca |
| Language: | English |
| Subjects: | Airway inflammation, Antibiotic adjuvant, Bactericidal effect, Carbohydrate-based biomaterials, Intranasal drug delivery, Respiratory pathogens, |
| Online Access: | https://research.wur.nl/en/publications/galacto-oligosaccharides-as-an-anti-bacterial-and-anti-invasive-a |
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dig-wur-nl-wurpubs-5958252024-12-04 Cai, Yang van Putten, Jos P.M. Gilbert, Myrthe S. Gerrits, Walter J.J. Folkerts, Gert Braber, Saskia Article/Letter to editor Biomaterials 283 (2022) ISSN: 0142-9612 Galacto-oligosaccharides as an anti-bacterial and anti-invasive agent in lung infections 2022 Emerging antimicrobial resistance in infections asks for novel intervention strategies. Galacto-oligosaccharides (GOS) might be attractive alternatives to antibiotics due to their anti-inflammatory and anti-adhesive properties. Mannheimia haemolytica is one of the major Pasteurellaceae associated with bovine lung infections. Using M. haemolytica, we demonstrated that GOS have the capacity to reduce bacterial viability and can be used as adjuvant to improve antibiotic efficacy. Using M. haemolytica-treated primary bronchial epithelial cells (PBECs) of calves, we identified the anti-adhesive and anti-invasive activities of GOS. The observed inhibition of cytokine/chemokine release and the prevention of airway epithelial barrier dysfunction in M. haemolytica-treated PBECs by GOS might be related to the downregulation of “toll-like receptor 4/nuclear factor-κB” pathway and the anti-invasive and anti-adhesive properties of GOS. Particularly, GOS lowered lipopolysaccharides- but not flagellin-induced cytokine/chemokine release in calf and human airway epithelial cells. Finally, we performed in vivo experiments in calves and demonstrated for the first time that intranasal application of GOS can relieve lung infections/inflammation and lower M. haemolytica positivity in the lungs without affecting clinical performance. These findings not only shed light on the anti-inflammatory mechanisms of GOS during lung infections, but GOS might also be a promising anti-bacterial agent for preventing (lung) infections. en application/pdf https://research.wur.nl/en/publications/galacto-oligosaccharides-as-an-anti-bacterial-and-anti-invasive-a 10.1016/j.biomaterials.2022.121461 https://edepot.wur.nl/567275 Airway inflammation Antibiotic adjuvant Bactericidal effect Carbohydrate-based biomaterials Intranasal drug delivery Respiratory pathogens https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/ Wageningen University & Research |
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Airway inflammation Antibiotic adjuvant Bactericidal effect Carbohydrate-based biomaterials Intranasal drug delivery Respiratory pathogens Airway inflammation Antibiotic adjuvant Bactericidal effect Carbohydrate-based biomaterials Intranasal drug delivery Respiratory pathogens |
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Airway inflammation Antibiotic adjuvant Bactericidal effect Carbohydrate-based biomaterials Intranasal drug delivery Respiratory pathogens Airway inflammation Antibiotic adjuvant Bactericidal effect Carbohydrate-based biomaterials Intranasal drug delivery Respiratory pathogens Cai, Yang van Putten, Jos P.M. Gilbert, Myrthe S. Gerrits, Walter J.J. Folkerts, Gert Braber, Saskia Galacto-oligosaccharides as an anti-bacterial and anti-invasive agent in lung infections |
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Emerging antimicrobial resistance in infections asks for novel intervention strategies. Galacto-oligosaccharides (GOS) might be attractive alternatives to antibiotics due to their anti-inflammatory and anti-adhesive properties. Mannheimia haemolytica is one of the major Pasteurellaceae associated with bovine lung infections. Using M. haemolytica, we demonstrated that GOS have the capacity to reduce bacterial viability and can be used as adjuvant to improve antibiotic efficacy. Using M. haemolytica-treated primary bronchial epithelial cells (PBECs) of calves, we identified the anti-adhesive and anti-invasive activities of GOS. The observed inhibition of cytokine/chemokine release and the prevention of airway epithelial barrier dysfunction in M. haemolytica-treated PBECs by GOS might be related to the downregulation of “toll-like receptor 4/nuclear factor-κB” pathway and the anti-invasive and anti-adhesive properties of GOS. Particularly, GOS lowered lipopolysaccharides- but not flagellin-induced cytokine/chemokine release in calf and human airway epithelial cells. Finally, we performed in vivo experiments in calves and demonstrated for the first time that intranasal application of GOS can relieve lung infections/inflammation and lower M. haemolytica positivity in the lungs without affecting clinical performance. These findings not only shed light on the anti-inflammatory mechanisms of GOS during lung infections, but GOS might also be a promising anti-bacterial agent for preventing (lung) infections. |
| format |
Article/Letter to editor |
| topic_facet |
Airway inflammation Antibiotic adjuvant Bactericidal effect Carbohydrate-based biomaterials Intranasal drug delivery Respiratory pathogens |
| author |
Cai, Yang van Putten, Jos P.M. Gilbert, Myrthe S. Gerrits, Walter J.J. Folkerts, Gert Braber, Saskia |
| author_facet |
Cai, Yang van Putten, Jos P.M. Gilbert, Myrthe S. Gerrits, Walter J.J. Folkerts, Gert Braber, Saskia |
| author_sort |
Cai, Yang |
| title |
Galacto-oligosaccharides as an anti-bacterial and anti-invasive agent in lung infections |
| title_short |
Galacto-oligosaccharides as an anti-bacterial and anti-invasive agent in lung infections |
| title_full |
Galacto-oligosaccharides as an anti-bacterial and anti-invasive agent in lung infections |
| title_fullStr |
Galacto-oligosaccharides as an anti-bacterial and anti-invasive agent in lung infections |
| title_full_unstemmed |
Galacto-oligosaccharides as an anti-bacterial and anti-invasive agent in lung infections |
| title_sort |
galacto-oligosaccharides as an anti-bacterial and anti-invasive agent in lung infections |
| url |
https://research.wur.nl/en/publications/galacto-oligosaccharides-as-an-anti-bacterial-and-anti-invasive-a |
| work_keys_str_mv |
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| _version_ |
1819142857379807232 |