Is nitric oxide a critical key factor in ABA-induced stomatal closure?
The role of nitric oxide (NO) in abscisic acid (ABA)-induced stomatal closure is a matter of debate. We conducted experiments in Vicia faba leaves using NO gas and sodium nitroprusside (SNP), a NO-donor compound, and compared their effects to those of ABA. In epidermal strips, stomatal closure was induced by ABA but not by NO, casting doubt on the role of NO in ABA-mediated stomatal closure. Leaf discs and intact leaves showed a dual dose response to NO: stomatal aperture widened at low dosage and narrowed at high dosage. Overcoming stomatal resistance by means of high CO2 concentration ([CO2]) restored photosynthesis in ABA-treated leaf discs but not in those exposed to NO. NO inhibited photosynthesis immediately, causing an instantaneous increase in intercellular [CO2] (Ci), followed by stomatal closure. However, lowering Ci by using low ambient [CO2] showed that it was not the main factor in NO-induced stomatal closure. In intact leaves, the rate of stomatal closure in response to NO was about one order of magnitude less than after ABA application. Because of the different kinetics of photosynthesis and stomatal closure that were observed, we conclude that NO is not likely to be the key factor in ABA-induced rapid stomatal closure, but that it fine-tunes stomatal aperture via different pathways.
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Format: | Article/Letter to editor biblioteca |
Language: | English |
Subjects: | ABA, epidermal peels, nitric oxide gas, photosynthesis, sodium nitroprusside (SNP), stomatal closure, |
Online Access: | https://research.wur.nl/en/publications/is-nitric-oxide-a-critical-key-factor-in-aba-induced-stomatal-clo |
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dig-wur-nl-wurpubs-5574882024-10-02 van Meeteren, U. Kaiser, M.E. Malcolm Matamoros, P.R. Verdonk, J.C. Aliniaeifard, Sasan Article/Letter to editor Journal of Experimental Botany 71 (2020) 1 ISSN: 0022-0957 Is nitric oxide a critical key factor in ABA-induced stomatal closure? 2020 The role of nitric oxide (NO) in abscisic acid (ABA)-induced stomatal closure is a matter of debate. We conducted experiments in Vicia faba leaves using NO gas and sodium nitroprusside (SNP), a NO-donor compound, and compared their effects to those of ABA. In epidermal strips, stomatal closure was induced by ABA but not by NO, casting doubt on the role of NO in ABA-mediated stomatal closure. Leaf discs and intact leaves showed a dual dose response to NO: stomatal aperture widened at low dosage and narrowed at high dosage. Overcoming stomatal resistance by means of high CO2 concentration ([CO2]) restored photosynthesis in ABA-treated leaf discs but not in those exposed to NO. NO inhibited photosynthesis immediately, causing an instantaneous increase in intercellular [CO2] (Ci), followed by stomatal closure. However, lowering Ci by using low ambient [CO2] showed that it was not the main factor in NO-induced stomatal closure. In intact leaves, the rate of stomatal closure in response to NO was about one order of magnitude less than after ABA application. Because of the different kinetics of photosynthesis and stomatal closure that were observed, we conclude that NO is not likely to be the key factor in ABA-induced rapid stomatal closure, but that it fine-tunes stomatal aperture via different pathways. en text/html https://research.wur.nl/en/publications/is-nitric-oxide-a-critical-key-factor-in-aba-induced-stomatal-clo 10.1093/jxb/erz437 https://edepot.wur.nl/509782 ABA epidermal peels nitric oxide gas photosynthesis sodium nitroprusside (SNP) stomatal closure https://creativecommons.org/licenses/by-nc/4.0/ Wageningen University & Research |
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ABA epidermal peels nitric oxide gas photosynthesis sodium nitroprusside (SNP) stomatal closure ABA epidermal peels nitric oxide gas photosynthesis sodium nitroprusside (SNP) stomatal closure |
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ABA epidermal peels nitric oxide gas photosynthesis sodium nitroprusside (SNP) stomatal closure ABA epidermal peels nitric oxide gas photosynthesis sodium nitroprusside (SNP) stomatal closure van Meeteren, U. Kaiser, M.E. Malcolm Matamoros, P.R. Verdonk, J.C. Aliniaeifard, Sasan Is nitric oxide a critical key factor in ABA-induced stomatal closure? |
description |
The role of nitric oxide (NO) in abscisic acid (ABA)-induced stomatal closure is a matter of debate. We conducted experiments in Vicia faba leaves using NO gas and sodium nitroprusside (SNP), a NO-donor compound, and compared their effects to those of ABA. In epidermal strips, stomatal closure was induced by ABA but not by NO, casting doubt on the role of NO in ABA-mediated stomatal closure. Leaf discs and intact leaves showed a dual dose response to NO: stomatal aperture widened at low dosage and narrowed at high dosage. Overcoming stomatal resistance by means of high CO2 concentration ([CO2]) restored photosynthesis in ABA-treated leaf discs but not in those exposed to NO. NO inhibited photosynthesis immediately, causing an instantaneous increase in intercellular [CO2] (Ci), followed by stomatal closure. However, lowering Ci by using low ambient [CO2] showed that it was not the main factor in NO-induced stomatal closure. In intact leaves, the rate of stomatal closure in response to NO was about one order of magnitude less than after ABA application. Because of the different kinetics of photosynthesis and stomatal closure that were observed, we conclude that NO is not likely to be the key factor in ABA-induced rapid stomatal closure, but that it fine-tunes stomatal aperture via different pathways. |
format |
Article/Letter to editor |
topic_facet |
ABA epidermal peels nitric oxide gas photosynthesis sodium nitroprusside (SNP) stomatal closure |
author |
van Meeteren, U. Kaiser, M.E. Malcolm Matamoros, P.R. Verdonk, J.C. Aliniaeifard, Sasan |
author_facet |
van Meeteren, U. Kaiser, M.E. Malcolm Matamoros, P.R. Verdonk, J.C. Aliniaeifard, Sasan |
author_sort |
van Meeteren, U. |
title |
Is nitric oxide a critical key factor in ABA-induced stomatal closure? |
title_short |
Is nitric oxide a critical key factor in ABA-induced stomatal closure? |
title_full |
Is nitric oxide a critical key factor in ABA-induced stomatal closure? |
title_fullStr |
Is nitric oxide a critical key factor in ABA-induced stomatal closure? |
title_full_unstemmed |
Is nitric oxide a critical key factor in ABA-induced stomatal closure? |
title_sort |
is nitric oxide a critical key factor in aba-induced stomatal closure? |
url |
https://research.wur.nl/en/publications/is-nitric-oxide-a-critical-key-factor-in-aba-induced-stomatal-clo |
work_keys_str_mv |
AT vanmeeterenu isnitricoxideacriticalkeyfactorinabainducedstomatalclosure AT kaiserme isnitricoxideacriticalkeyfactorinabainducedstomatalclosure AT malcolmmatamorospr isnitricoxideacriticalkeyfactorinabainducedstomatalclosure AT verdonkjc isnitricoxideacriticalkeyfactorinabainducedstomatalclosure AT aliniaeifardsasan isnitricoxideacriticalkeyfactorinabainducedstomatalclosure |
_version_ |
1813196809867100160 |