Transcription profiling by array of barley after inoculation with Puccinia hordei, Puccinia triticina or mock-inoculation to identify candidate genes for nonhost resistance against rust disease

Transcription profiling by array of barley after inoculation with Puccinia hordei, Puccinia triticina or mock-inoculation to identify candidate genes for nonhost resistance against rust disease

In this experiment the transcriptome reprogramming in barley during host and nonhost interaction with Puccinia sp. was analyzed in a time-series approach. Ten days old barley plants of cv. Vada were mock-inoculated or inoculated with P. hordei (Ph), 1.2.1 isolate, or P. triticina (Pt), BRW96258 isolate. After 12, 24, 36 and 48 hours first leaves were sampled. Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany), the Ambion TURBO DNA-free DNase Kit was used for DNA elimination, and RNA was hybridized to Agilent 44k oligonucleotide arrays.

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Bibliographic Details
Main Authors: Delventhal, Rhoda, Niks, R.E.
Format: Dataset biblioteca
Published: RWTH Aachen University
Subjects:Hordeum vulgare,
Online Access:https://research.wur.nl/en/datasets/transcription-profiling-by-array-of-barley-after-inoculation-with
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spelling dig-wur-nl-wurpubs-5552482024-09-23 Delventhal, Rhoda Niks, R.E. Dataset Transcription profiling by array of barley after inoculation with Puccinia hordei, Puccinia triticina or mock-inoculation to identify candidate genes for nonhost resistance against rust disease 2017 In this experiment the transcriptome reprogramming in barley during host and nonhost interaction with Puccinia sp. was analyzed in a time-series approach. Ten days old barley plants of cv. Vada were mock-inoculated or inoculated with P. hordei (Ph), 1.2.1 isolate, or P. triticina (Pt), BRW96258 isolate. After 12, 24, 36 and 48 hours first leaves were sampled. Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany), the Ambion TURBO DNA-free DNase Kit was used for DNA elimination, and RNA was hybridized to Agilent 44k oligonucleotide arrays. RWTH Aachen University text/html https://research.wur.nl/en/datasets/transcription-profiling-by-array-of-barley-after-inoculation-with https://edepot.wur.nl/504792 Hordeum vulgare Wageningen University & Research
institution WUR NL
collection DSpace
country Países bajos
countrycode NL
component Bibliográfico
access En linea
databasecode dig-wur-nl
tag biblioteca
region Europa del Oeste
libraryname WUR Library Netherlands
topic Hordeum vulgare
Hordeum vulgare
spellingShingle Hordeum vulgare
Hordeum vulgare
Delventhal, Rhoda
Niks, R.E.
Transcription profiling by array of barley after inoculation with Puccinia hordei, Puccinia triticina or mock-inoculation to identify candidate genes for nonhost resistance against rust disease
description In this experiment the transcriptome reprogramming in barley during host and nonhost interaction with Puccinia sp. was analyzed in a time-series approach. Ten days old barley plants of cv. Vada were mock-inoculated or inoculated with P. hordei (Ph), 1.2.1 isolate, or P. triticina (Pt), BRW96258 isolate. After 12, 24, 36 and 48 hours first leaves were sampled. Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany), the Ambion TURBO DNA-free DNase Kit was used for DNA elimination, and RNA was hybridized to Agilent 44k oligonucleotide arrays.
format Dataset
topic_facet Hordeum vulgare
author Delventhal, Rhoda
Niks, R.E.
author_facet Delventhal, Rhoda
Niks, R.E.
author_sort Delventhal, Rhoda
title Transcription profiling by array of barley after inoculation with Puccinia hordei, Puccinia triticina or mock-inoculation to identify candidate genes for nonhost resistance against rust disease
title_short Transcription profiling by array of barley after inoculation with Puccinia hordei, Puccinia triticina or mock-inoculation to identify candidate genes for nonhost resistance against rust disease
title_full Transcription profiling by array of barley after inoculation with Puccinia hordei, Puccinia triticina or mock-inoculation to identify candidate genes for nonhost resistance against rust disease
title_fullStr Transcription profiling by array of barley after inoculation with Puccinia hordei, Puccinia triticina or mock-inoculation to identify candidate genes for nonhost resistance against rust disease
title_full_unstemmed Transcription profiling by array of barley after inoculation with Puccinia hordei, Puccinia triticina or mock-inoculation to identify candidate genes for nonhost resistance against rust disease
title_sort transcription profiling by array of barley after inoculation with puccinia hordei, puccinia triticina or mock-inoculation to identify candidate genes for nonhost resistance against rust disease
publisher RWTH Aachen University
url https://research.wur.nl/en/datasets/transcription-profiling-by-array-of-barley-after-inoculation-with
work_keys_str_mv AT delventhalrhoda transcriptionprofilingbyarrayofbarleyafterinoculationwithpucciniahordeipucciniatriticinaormockinoculationtoidentifycandidategenesfornonhostresistanceagainstrustdisease
AT niksre transcriptionprofilingbyarrayofbarleyafterinoculationwithpucciniahordeipucciniatriticinaormockinoculationtoidentifycandidategenesfornonhostresistanceagainstrustdisease
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