Marginal selenium deficiency down-regulates inflammation-related genes in splenic leukocytes of the mouse

Moderate selenium deficiency may lead to an impaired capacity to cope with health challenges. Functional effects of suboptimal selenium intake are not fully known, and biomarkers for an insufficient selenium supply are inadequate. We therefore fed mice diets of moderately deficient or adequate selenium intake for 6 weeks. Changes in global gene expression were monitored by microarray analysis in splenic leukocytes. Genes for four selenoproteins, Sepw1, Gpx1, Selh and Sep15, were the most significantly down-regulated in moderate selenium deficiency, and this was confirmed by quantitative polymerase chain reaction (qPCR). Classification of significantly affected genes revealed that processes related to inflammation, heme biosynthesis, DNA replication and transcription, cell cycle and transport were affected by selenium restriction. Down-regulation by moderate selenium deficiency of specific genes involved in inflammation and heme biosynthesis was confirmed by qPCR. Myeloperoxidase and lysozyme activities were decreased in selenium-restricted leukocytes, providing evidence for functional consequences. Genes for 31 nuclear factor (NF)-κB targets were down-regulated in moderate selenium deficiency, indicating an impaired NF-κB signaling. Together, the observed changes point to a disturbance in inflammatory response. The selenoproteins found here to be sensitive to selenium intake in murine leukocytes might also be useful as biomarkers for a moderate selenium deficiency in humans.

Saved in:
Bibliographic Details
Main Authors: Kipp, A.P., Banning, A., Brigelius, R., Keijer, J., van Schothorst, E.M.
Format: Dataset biblioteca
Published: Wageningen University
Subjects:Mus musculus,
Online Access:https://research.wur.nl/en/datasets/marginal-selenium-deficiency-down-regulates-inflammation-related-
Tags: Add Tag
No Tags, Be the first to tag this record!
id dig-wur-nl-wurpubs-543844
record_format koha
spelling dig-wur-nl-wurpubs-5438442024-09-30 Kipp, A.P. Banning, A. Brigelius, R. Keijer, J. van Schothorst, E.M. Dataset Marginal selenium deficiency down-regulates inflammation-related genes in splenic leukocytes of the mouse 2018 Moderate selenium deficiency may lead to an impaired capacity to cope with health challenges. Functional effects of suboptimal selenium intake are not fully known, and biomarkers for an insufficient selenium supply are inadequate. We therefore fed mice diets of moderately deficient or adequate selenium intake for 6 weeks. Changes in global gene expression were monitored by microarray analysis in splenic leukocytes. Genes for four selenoproteins, Sepw1, Gpx1, Selh and Sep15, were the most significantly down-regulated in moderate selenium deficiency, and this was confirmed by quantitative polymerase chain reaction (qPCR). Classification of significantly affected genes revealed that processes related to inflammation, heme biosynthesis, DNA replication and transcription, cell cycle and transport were affected by selenium restriction. Down-regulation by moderate selenium deficiency of specific genes involved in inflammation and heme biosynthesis was confirmed by qPCR. Myeloperoxidase and lysozyme activities were decreased in selenium-restricted leukocytes, providing evidence for functional consequences. Genes for 31 nuclear factor (NF)-κB targets were down-regulated in moderate selenium deficiency, indicating an impaired NF-κB signaling. Together, the observed changes point to a disturbance in inflammatory response. The selenoproteins found here to be sensitive to selenium intake in murine leukocytes might also be useful as biomarkers for a moderate selenium deficiency in humans. Wageningen University text/html https://research.wur.nl/en/datasets/marginal-selenium-deficiency-down-regulates-inflammation-related- https://edepot.wur.nl/465306 Mus musculus Wageningen University & Research
institution WUR NL
collection DSpace
country Países bajos
countrycode NL
component Bibliográfico
access En linea
databasecode dig-wur-nl
tag biblioteca
region Europa del Oeste
libraryname WUR Library Netherlands
topic Mus musculus
Mus musculus
spellingShingle Mus musculus
Mus musculus
Kipp, A.P.
Banning, A.
Brigelius, R.
Keijer, J.
van Schothorst, E.M.
Marginal selenium deficiency down-regulates inflammation-related genes in splenic leukocytes of the mouse
description Moderate selenium deficiency may lead to an impaired capacity to cope with health challenges. Functional effects of suboptimal selenium intake are not fully known, and biomarkers for an insufficient selenium supply are inadequate. We therefore fed mice diets of moderately deficient or adequate selenium intake for 6 weeks. Changes in global gene expression were monitored by microarray analysis in splenic leukocytes. Genes for four selenoproteins, Sepw1, Gpx1, Selh and Sep15, were the most significantly down-regulated in moderate selenium deficiency, and this was confirmed by quantitative polymerase chain reaction (qPCR). Classification of significantly affected genes revealed that processes related to inflammation, heme biosynthesis, DNA replication and transcription, cell cycle and transport were affected by selenium restriction. Down-regulation by moderate selenium deficiency of specific genes involved in inflammation and heme biosynthesis was confirmed by qPCR. Myeloperoxidase and lysozyme activities were decreased in selenium-restricted leukocytes, providing evidence for functional consequences. Genes for 31 nuclear factor (NF)-κB targets were down-regulated in moderate selenium deficiency, indicating an impaired NF-κB signaling. Together, the observed changes point to a disturbance in inflammatory response. The selenoproteins found here to be sensitive to selenium intake in murine leukocytes might also be useful as biomarkers for a moderate selenium deficiency in humans.
format Dataset
topic_facet Mus musculus
author Kipp, A.P.
Banning, A.
Brigelius, R.
Keijer, J.
van Schothorst, E.M.
author_facet Kipp, A.P.
Banning, A.
Brigelius, R.
Keijer, J.
van Schothorst, E.M.
author_sort Kipp, A.P.
title Marginal selenium deficiency down-regulates inflammation-related genes in splenic leukocytes of the mouse
title_short Marginal selenium deficiency down-regulates inflammation-related genes in splenic leukocytes of the mouse
title_full Marginal selenium deficiency down-regulates inflammation-related genes in splenic leukocytes of the mouse
title_fullStr Marginal selenium deficiency down-regulates inflammation-related genes in splenic leukocytes of the mouse
title_full_unstemmed Marginal selenium deficiency down-regulates inflammation-related genes in splenic leukocytes of the mouse
title_sort marginal selenium deficiency down-regulates inflammation-related genes in splenic leukocytes of the mouse
publisher Wageningen University
url https://research.wur.nl/en/datasets/marginal-selenium-deficiency-down-regulates-inflammation-related-
work_keys_str_mv AT kippap marginalseleniumdeficiencydownregulatesinflammationrelatedgenesinsplenicleukocytesofthemouse
AT banninga marginalseleniumdeficiencydownregulatesinflammationrelatedgenesinsplenicleukocytesofthemouse
AT brigeliusr marginalseleniumdeficiencydownregulatesinflammationrelatedgenesinsplenicleukocytesofthemouse
AT keijerj marginalseleniumdeficiencydownregulatesinflammationrelatedgenesinsplenicleukocytesofthemouse
AT vanschothorstem marginalseleniumdeficiencydownregulatesinflammationrelatedgenesinsplenicleukocytesofthemouse
_version_ 1816156750689075200