Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection

Objective: Loop-mediated isothermal amplification (LAMP) of DNA is gaining relevance as a method to detect nucleic acids, as it is easier, faster, and more powerful than conventional Polymerase Chain Reaction. However, LAMP is still mostly used in laboratory settings, because of the lack of a cheap and easy, one-button device that can perform LAMP experiments. Results: Here we show how to build and program an Arduino shield for a LAMP and detection of DNA. The here described Arduino Shield is cheap, easy to assemble, to program and use, it is battery operated and the detection of DNA is done by naked-eye so that it can be used in field.

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Bibliographic Details
Main Authors: Velders, Aldrik H., Schoen, Cor, Saggiomo, Vittorio
Format: Article/Letter to editor biblioteca
Language:English
Subjects:Arduino instruments, Loop mediated isothermal amplification, Nucleic acid detection, Portable,
Online Access:https://research.wur.nl/en/publications/loop-mediated-isothermal-amplification-lamp-shield-for-arduino-dn
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spelling dig-wur-nl-wurpubs-5340772024-12-04 Velders, Aldrik H. Schoen, Cor Saggiomo, Vittorio Article/Letter to editor BMC Research Notes 11 (2018) ISSN: 1756-0500 Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection 2018 Objective: Loop-mediated isothermal amplification (LAMP) of DNA is gaining relevance as a method to detect nucleic acids, as it is easier, faster, and more powerful than conventional Polymerase Chain Reaction. However, LAMP is still mostly used in laboratory settings, because of the lack of a cheap and easy, one-button device that can perform LAMP experiments. Results: Here we show how to build and program an Arduino shield for a LAMP and detection of DNA. The here described Arduino Shield is cheap, easy to assemble, to program and use, it is battery operated and the detection of DNA is done by naked-eye so that it can be used in field. en application/pdf https://research.wur.nl/en/publications/loop-mediated-isothermal-amplification-lamp-shield-for-arduino-dn 10.1186/s13104-018-3197-9 https://edepot.wur.nl/441950 Arduino instruments Loop mediated isothermal amplification Nucleic acid detection Portable https://creativecommons.org/licenses/by/4.0/ Wageningen University & Research
institution WUR NL
collection DSpace
country Países bajos
countrycode NL
component Bibliográfico
access En linea
databasecode dig-wur-nl
tag biblioteca
region Europa del Oeste
libraryname WUR Library Netherlands
language English
topic Arduino instruments
Loop mediated isothermal amplification
Nucleic acid detection
Portable
Arduino instruments
Loop mediated isothermal amplification
Nucleic acid detection
Portable
spellingShingle Arduino instruments
Loop mediated isothermal amplification
Nucleic acid detection
Portable
Arduino instruments
Loop mediated isothermal amplification
Nucleic acid detection
Portable
Velders, Aldrik H.
Schoen, Cor
Saggiomo, Vittorio
Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection
description Objective: Loop-mediated isothermal amplification (LAMP) of DNA is gaining relevance as a method to detect nucleic acids, as it is easier, faster, and more powerful than conventional Polymerase Chain Reaction. However, LAMP is still mostly used in laboratory settings, because of the lack of a cheap and easy, one-button device that can perform LAMP experiments. Results: Here we show how to build and program an Arduino shield for a LAMP and detection of DNA. The here described Arduino Shield is cheap, easy to assemble, to program and use, it is battery operated and the detection of DNA is done by naked-eye so that it can be used in field.
format Article/Letter to editor
topic_facet Arduino instruments
Loop mediated isothermal amplification
Nucleic acid detection
Portable
author Velders, Aldrik H.
Schoen, Cor
Saggiomo, Vittorio
author_facet Velders, Aldrik H.
Schoen, Cor
Saggiomo, Vittorio
author_sort Velders, Aldrik H.
title Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection
title_short Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection
title_full Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection
title_fullStr Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection
title_full_unstemmed Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection
title_sort loop-mediated isothermal amplification (lamp) shield for arduino dna detection
url https://research.wur.nl/en/publications/loop-mediated-isothermal-amplification-lamp-shield-for-arduino-dn
work_keys_str_mv AT veldersaldrikh loopmediatedisothermalamplificationlampshieldforarduinodnadetection
AT schoencor loopmediatedisothermalamplificationlampshieldforarduinodnadetection
AT saggiomovittorio loopmediatedisothermalamplificationlampshieldforarduinodnadetection
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