Liver X receptor beta is the dominant LXR subtype in skeletal muscle
Liver X receptors (LXRs) are important regulators of cholesterol, lipid and glucose metabolism and have been extensively studied in liver, macrophages and adipose tissue. However, their role in skeletal muscle is not yet fully elucidated and the functional role of each of the LXRα and LXRβ subtypes in skeletal muscle is at present unknown. To study the importance of each of the receptor subtypes, myotube cultures derived from wild type (WT), LXRα and LXRβ knockout (KO) mice were established. The present study shows that treatment with the unselective LXR agonist T0901317 increased mRNA levels of LXR target genes such as sterol regulatory element-binding transcription factor 1 (SREBF1), fatty acid synthase (FASN), stearoyl-CoA desaturase 1 (SCD1) and ATP-binding cassette transporter A1 (ABCA1) in myotubes established from WT and LXRα KO mice. However, only minor changes in expression level were observed for these genes after treatment with T0901317 in myotubes from LXRβ KO mice. Gene expression analysis using Affymetrix NuGO Genechip arrays showed that few other genes than the classical, well known LXR target genes were regulated by LXR in skeletal muscle. Furthermore, functional studies using radiolabeled substrates showed that treatment with T0901317 increased lipogenesis and apoA1 dependent cholesterol efflux, in myotubes from WT and LXRα KO mice, but not LXRβ KO mice. The data suggest that the lipogenic effects of LXRs, as well as the LXR-stimulated cholesterol efflux, are mainly mediated by LXRβ in skeletal muscle.
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Dataset biblioteca |
| Published: |
Wageningen University
|
| Subjects: | Mus musculus, |
| Online Access: | https://research.wur.nl/en/datasets/liver-x-receptor-beta-is-the-dominant-lxr-subtype-in-skeletal-mus |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-wur-nl-wurpubs-530933 |
|---|---|
| record_format |
koha |
| spelling |
dig-wur-nl-wurpubs-5309332024-09-30 Hessvik, Nina P. Boekschoten, Mark Baltzersen, Mari-Ann Kersten, Sander Xu, Xiufeng Andersen, Harriet Rustan, Arild C. Thoresen, Hege Dataset Liver X receptor beta is the dominant LXR subtype in skeletal muscle 2010 Liver X receptors (LXRs) are important regulators of cholesterol, lipid and glucose metabolism and have been extensively studied in liver, macrophages and adipose tissue. However, their role in skeletal muscle is not yet fully elucidated and the functional role of each of the LXRα and LXRβ subtypes in skeletal muscle is at present unknown. To study the importance of each of the receptor subtypes, myotube cultures derived from wild type (WT), LXRα and LXRβ knockout (KO) mice were established. The present study shows that treatment with the unselective LXR agonist T0901317 increased mRNA levels of LXR target genes such as sterol regulatory element-binding transcription factor 1 (SREBF1), fatty acid synthase (FASN), stearoyl-CoA desaturase 1 (SCD1) and ATP-binding cassette transporter A1 (ABCA1) in myotubes established from WT and LXRα KO mice. However, only minor changes in expression level were observed for these genes after treatment with T0901317 in myotubes from LXRβ KO mice. Gene expression analysis using Affymetrix NuGO Genechip arrays showed that few other genes than the classical, well known LXR target genes were regulated by LXR in skeletal muscle. Furthermore, functional studies using radiolabeled substrates showed that treatment with T0901317 increased lipogenesis and apoA1 dependent cholesterol efflux, in myotubes from WT and LXRα KO mice, but not LXRβ KO mice. The data suggest that the lipogenic effects of LXRs, as well as the LXR-stimulated cholesterol efflux, are mainly mediated by LXRβ in skeletal muscle. Liver X receptors (LXRs) are important regulators of cholesterol, lipid and glucose metabolism and have been extensively studied in liver, macrophages and adipose tissue. However, their role in skeletal muscle is not yet fully elucidated and the functional role of each of the LXRα and LXRβ subtypes in skeletal muscle is at present unknown. To study the importance of each of the receptor subtypes, myotube cultures derived from wild type (WT), LXRα and LXRβ knockout (KO) mice were established. The present study shows that treatment with the unselective LXR agonist T0901317 increased mRNA levels of LXR target genes such as sterol regulatory element-binding transcription factor 1 (SREBF1), fatty acid synthase (FASN), stearoyl-CoA desaturase 1 (SCD1) and ATP-binding cassette transporter A1 (ABCA1) in myotubes established from WT and LXRα KO mice. However, only minor changes in expression level were observed for these genes after treatment with T0901317 in myotubes from LXRβ KO mice. Gene expression analysis using Affymetrix NuGO Genechip arrays showed that few other genes than the classical, well known LXR target genes were regulated by LXR in skeletal muscle. Furthermore, functional studies using radiolabeled substrates showed that treatment with T0901317 increased lipogenesis and apoA1 dependent cholesterol efflux, in myotubes from WT and LXRα KO mice, but not LXRβ KO mice. The data suggest that the lipogenic effects of LXRs, as well as the LXR-stimulated cholesterol efflux, are mainly mediated by LXRβ in skeletal muscle. Wageningen University text/html https://research.wur.nl/en/datasets/liver-x-receptor-beta-is-the-dominant-lxr-subtype-in-skeletal-mus https://edepot.wur.nl/429720 Mus musculus Mus musculus Wageningen University & Research |
| institution |
WUR NL |
| collection |
DSpace |
| country |
Países bajos |
| countrycode |
NL |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-wur-nl |
| tag |
biblioteca |
| region |
Europa del Oeste |
| libraryname |
WUR Library Netherlands |
| topic |
Mus musculus Mus musculus Mus musculus Mus musculus |
| spellingShingle |
Mus musculus Mus musculus Mus musculus Mus musculus Hessvik, Nina P. Boekschoten, Mark Baltzersen, Mari-Ann Kersten, Sander Xu, Xiufeng Andersen, Harriet Rustan, Arild C. Thoresen, Hege Liver X receptor beta is the dominant LXR subtype in skeletal muscle |
| description |
Liver X receptors (LXRs) are important regulators of cholesterol, lipid and glucose metabolism and have been extensively studied in liver, macrophages and adipose tissue. However, their role in skeletal muscle is not yet fully elucidated and the functional role of each of the LXRα and LXRβ subtypes in skeletal muscle is at present unknown. To study the importance of each of the receptor subtypes, myotube cultures derived from wild type (WT), LXRα and LXRβ knockout (KO) mice were established. The present study shows that treatment with the unselective LXR agonist T0901317 increased mRNA levels of LXR target genes such as sterol regulatory element-binding transcription factor 1 (SREBF1), fatty acid synthase (FASN), stearoyl-CoA desaturase 1 (SCD1) and ATP-binding cassette transporter A1 (ABCA1) in myotubes established from WT and LXRα KO mice. However, only minor changes in expression level were observed for these genes after treatment with T0901317 in myotubes from LXRβ KO mice. Gene expression analysis using Affymetrix NuGO Genechip arrays showed that few other genes than the classical, well known LXR target genes were regulated by LXR in skeletal muscle. Furthermore, functional studies using radiolabeled substrates showed that treatment with T0901317 increased lipogenesis and apoA1 dependent cholesterol efflux, in myotubes from WT and LXRα KO mice, but not LXRβ KO mice. The data suggest that the lipogenic effects of LXRs, as well as the LXR-stimulated cholesterol efflux, are mainly mediated by LXRβ in skeletal muscle. |
| format |
Dataset |
| topic_facet |
Mus musculus Mus musculus |
| author |
Hessvik, Nina P. Boekschoten, Mark Baltzersen, Mari-Ann Kersten, Sander Xu, Xiufeng Andersen, Harriet Rustan, Arild C. Thoresen, Hege |
| author_facet |
Hessvik, Nina P. Boekschoten, Mark Baltzersen, Mari-Ann Kersten, Sander Xu, Xiufeng Andersen, Harriet Rustan, Arild C. Thoresen, Hege |
| author_sort |
Hessvik, Nina P. |
| title |
Liver X receptor beta is the dominant LXR subtype in skeletal muscle |
| title_short |
Liver X receptor beta is the dominant LXR subtype in skeletal muscle |
| title_full |
Liver X receptor beta is the dominant LXR subtype in skeletal muscle |
| title_fullStr |
Liver X receptor beta is the dominant LXR subtype in skeletal muscle |
| title_full_unstemmed |
Liver X receptor beta is the dominant LXR subtype in skeletal muscle |
| title_sort |
liver x receptor beta is the dominant lxr subtype in skeletal muscle |
| publisher |
Wageningen University |
| url |
https://research.wur.nl/en/datasets/liver-x-receptor-beta-is-the-dominant-lxr-subtype-in-skeletal-mus |
| work_keys_str_mv |
AT hessvikninap liverxreceptorbetaisthedominantlxrsubtypeinskeletalmuscle AT boekschotenmark liverxreceptorbetaisthedominantlxrsubtypeinskeletalmuscle AT baltzersenmariann liverxreceptorbetaisthedominantlxrsubtypeinskeletalmuscle AT kerstensander liverxreceptorbetaisthedominantlxrsubtypeinskeletalmuscle AT xuxiufeng liverxreceptorbetaisthedominantlxrsubtypeinskeletalmuscle AT andersenharriet liverxreceptorbetaisthedominantlxrsubtypeinskeletalmuscle AT rustanarildc liverxreceptorbetaisthedominantlxrsubtypeinskeletalmuscle AT thoresenhege liverxreceptorbetaisthedominantlxrsubtypeinskeletalmuscle |
| _version_ |
1816161579838734336 |