Comparative transcriptome analysis of biofilm and planktonic cells of Bacillus cereus ATCC 10987
Planktonic and biofilm cells of Bacillus cereus ATCC 14579 and ATCC 10987 were studied using microscopy and transcriptome analysis. By microscopy, clear differences could be observed between biofilm and planktonic cells as well as between the two strains. By using hierarchical clustering of the transcriptome data, little difference was observed between the biofilm cells of B. cereus ATCC 14579 and ATCC 10987. Different responses between biofilm and planktonic cells could be identified using transcriptome analysis. Biofilm formation seemed to cause a shift in metabolism with up- or down-regulation of genes involved in different metabolic pathways. Genes involved in motility were down-regulated. No clear up-regulation related to capsular or extracellular polysaccharides was observed. Sporulation was observed in biofilm cells using microscopy, which was corroborated with up-regulation of genes involved in sporulation in biofilm cells. The results obtained in this study provide insight in general and strain specific behavior of B. cereus cells in multicellular communities.
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dig-wur-nl-wurpubs-5298832024-06-25 Wijman, Janneke Mols, M. Tempelaars, Marcel Abee, Tjakko Dataset Comparative transcriptome analysis of biofilm and planktonic cells of Bacillus cereus ATCC 10987 2015 Planktonic and biofilm cells of Bacillus cereus ATCC 14579 and ATCC 10987 were studied using microscopy and transcriptome analysis. By microscopy, clear differences could be observed between biofilm and planktonic cells as well as between the two strains. By using hierarchical clustering of the transcriptome data, little difference was observed between the biofilm cells of B. cereus ATCC 14579 and ATCC 10987. Different responses between biofilm and planktonic cells could be identified using transcriptome analysis. Biofilm formation seemed to cause a shift in metabolism with up- or down-regulation of genes involved in different metabolic pathways. Genes involved in motility were down-regulated. No clear up-regulation related to capsular or extracellular polysaccharides was observed. Sporulation was observed in biofilm cells using microscopy, which was corroborated with up-regulation of genes involved in sporulation in biofilm cells. The results obtained in this study provide insight in general and strain specific behavior of B. cereus cells in multicellular communities. Planktonic and biofilm cells of Bacillus cereus ATCC 14579 and ATCC 10987 were studied using microscopy and transcriptome analysis. By microscopy, clear differences could be observed between biofilm and planktonic cells as well as between the two strains. By using hierarchical clustering of the transcriptome data, little difference was observed between the biofilm cells of B. cereus ATCC 14579 and ATCC 10987. Different responses between biofilm and planktonic cells could be identified using transcriptome analysis. Biofilm formation seemed to cause a shift in metabolism with up- or down-regulation of genes involved in different metabolic pathways. Genes involved in motility were down-regulated. No clear up-regulation related to capsular or extracellular polysaccharides was observed. Sporulation was observed in biofilm cells using microscopy, which was corroborated with up-regulation of genes involved in sporulation in biofilm cells. The results obtained in this study provide insight in general and strain specific behavior of B. cereus cells in multicellular communities. Wageningen UR text/html https://research.wur.nl/en/datasets/comparative-transcriptome-analysis-of-biofilm-and-planktonic-cell https://edepot.wur.nl/427571 Bacillus cereus ATCC 10987 Bacillus cereus Wageningen University & Research |
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Bacillus cereus ATCC 10987 Bacillus cereus Bacillus cereus ATCC 10987 Bacillus cereus |
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Bacillus cereus ATCC 10987 Bacillus cereus Bacillus cereus ATCC 10987 Bacillus cereus Wijman, Janneke Mols, M. Tempelaars, Marcel Abee, Tjakko Comparative transcriptome analysis of biofilm and planktonic cells of Bacillus cereus ATCC 10987 |
description |
Planktonic and biofilm cells of Bacillus cereus ATCC 14579 and ATCC 10987 were studied using microscopy and transcriptome analysis. By microscopy, clear differences could be observed between biofilm and planktonic cells as well as between the two strains. By using hierarchical clustering of the transcriptome data, little difference was observed between the biofilm cells of B. cereus ATCC 14579 and ATCC 10987. Different responses between biofilm and planktonic cells could be identified using transcriptome analysis. Biofilm formation seemed to cause a shift in metabolism with up- or down-regulation of genes involved in different metabolic pathways. Genes involved in motility were down-regulated. No clear up-regulation related to capsular or extracellular polysaccharides was observed. Sporulation was observed in biofilm cells using microscopy, which was corroborated with up-regulation of genes involved in sporulation in biofilm cells. The results obtained in this study provide insight in general and strain specific behavior of B. cereus cells in multicellular communities. |
format |
Dataset |
topic_facet |
Bacillus cereus ATCC 10987 Bacillus cereus |
author |
Wijman, Janneke Mols, M. Tempelaars, Marcel Abee, Tjakko |
author_facet |
Wijman, Janneke Mols, M. Tempelaars, Marcel Abee, Tjakko |
author_sort |
Wijman, Janneke |
title |
Comparative transcriptome analysis of biofilm and planktonic cells of Bacillus cereus ATCC 10987 |
title_short |
Comparative transcriptome analysis of biofilm and planktonic cells of Bacillus cereus ATCC 10987 |
title_full |
Comparative transcriptome analysis of biofilm and planktonic cells of Bacillus cereus ATCC 10987 |
title_fullStr |
Comparative transcriptome analysis of biofilm and planktonic cells of Bacillus cereus ATCC 10987 |
title_full_unstemmed |
Comparative transcriptome analysis of biofilm and planktonic cells of Bacillus cereus ATCC 10987 |
title_sort |
comparative transcriptome analysis of biofilm and planktonic cells of bacillus cereus atcc 10987 |
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Wageningen UR |
url |
https://research.wur.nl/en/datasets/comparative-transcriptome-analysis-of-biofilm-and-planktonic-cell |
work_keys_str_mv |
AT wijmanjanneke comparativetranscriptomeanalysisofbiofilmandplanktoniccellsofbacilluscereusatcc10987 AT molsm comparativetranscriptomeanalysisofbiofilmandplanktoniccellsofbacilluscereusatcc10987 AT tempelaarsmarcel comparativetranscriptomeanalysisofbiofilmandplanktoniccellsofbacilluscereusatcc10987 AT abeetjakko comparativetranscriptomeanalysisofbiofilmandplanktoniccellsofbacilluscereusatcc10987 |
_version_ |
1813201182863130624 |