Comparison of Protein Extracts from Various Unicellular Green Sources
Photosynthetic unicellular organisms are considered as promising alternative protein sources. The aim of this study is to understand the extent to which these green sources differ with respect to their gross composition and how these differences affect the final protein isolate. Using mild isolation techniques, proteins were extracted and isolated from four different unicellular sources (Arthrospira (spirulina) maxima, Nannochloropsis gaditana, Tetraselmis impellucida, and Scenedesmus dimorphus). Despite differences in protein contents of the sources (27-62% w/w) and in protein extractability (17-74% w/w), final protein isolates were obtained that had similar protein contents (62-77% w/w) and protein yields (3-9% w/w). Protein solubility as a function of pH was different between the sources and in ionic strength dependency, especially at pH < 4.0. Overall, the characterization and extraction protocol used allows a relatively fast and well-described isolation of purified proteins from novel protein sources.
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Format: | Article/Letter to editor biblioteca |
Language: | English |
Subjects: | Microalgae, amino acid composition, carbohydrate composition, cyanobacteria, physicochemical properties, single-cell protein, |
Online Access: | https://research.wur.nl/en/publications/comparison-of-protein-extracts-from-various-unicellular-green-sou |
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dig-wur-nl-wurpubs-5275122024-10-02 Teuling, Emma Wierenga, Peter A. Schrama, Johan W. Gruppen, Harry Article/Letter to editor Journal of Agricultural and Food Chemistry 65 (2017) 36 ISSN: 0021-8561 Comparison of Protein Extracts from Various Unicellular Green Sources 2017 Photosynthetic unicellular organisms are considered as promising alternative protein sources. The aim of this study is to understand the extent to which these green sources differ with respect to their gross composition and how these differences affect the final protein isolate. Using mild isolation techniques, proteins were extracted and isolated from four different unicellular sources (Arthrospira (spirulina) maxima, Nannochloropsis gaditana, Tetraselmis impellucida, and Scenedesmus dimorphus). Despite differences in protein contents of the sources (27-62% w/w) and in protein extractability (17-74% w/w), final protein isolates were obtained that had similar protein contents (62-77% w/w) and protein yields (3-9% w/w). Protein solubility as a function of pH was different between the sources and in ionic strength dependency, especially at pH < 4.0. Overall, the characterization and extraction protocol used allows a relatively fast and well-described isolation of purified proteins from novel protein sources. en application/pdf https://research.wur.nl/en/publications/comparison-of-protein-extracts-from-various-unicellular-green-sou 10.1021/acs.jafc.7b01788 https://edepot.wur.nl/424314 Microalgae amino acid composition carbohydrate composition cyanobacteria physicochemical properties single-cell protein https://creativecommons.org/licenses/by-nc-nd/4.0/ Wageningen University & Research |
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Microalgae amino acid composition carbohydrate composition cyanobacteria physicochemical properties single-cell protein Microalgae amino acid composition carbohydrate composition cyanobacteria physicochemical properties single-cell protein |
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Microalgae amino acid composition carbohydrate composition cyanobacteria physicochemical properties single-cell protein Microalgae amino acid composition carbohydrate composition cyanobacteria physicochemical properties single-cell protein Teuling, Emma Wierenga, Peter A. Schrama, Johan W. Gruppen, Harry Comparison of Protein Extracts from Various Unicellular Green Sources |
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Photosynthetic unicellular organisms are considered as promising alternative protein sources. The aim of this study is to understand the extent to which these green sources differ with respect to their gross composition and how these differences affect the final protein isolate. Using mild isolation techniques, proteins were extracted and isolated from four different unicellular sources (Arthrospira (spirulina) maxima, Nannochloropsis gaditana, Tetraselmis impellucida, and Scenedesmus dimorphus). Despite differences in protein contents of the sources (27-62% w/w) and in protein extractability (17-74% w/w), final protein isolates were obtained that had similar protein contents (62-77% w/w) and protein yields (3-9% w/w). Protein solubility as a function of pH was different between the sources and in ionic strength dependency, especially at pH < 4.0. Overall, the characterization and extraction protocol used allows a relatively fast and well-described isolation of purified proteins from novel protein sources. |
format |
Article/Letter to editor |
topic_facet |
Microalgae amino acid composition carbohydrate composition cyanobacteria physicochemical properties single-cell protein |
author |
Teuling, Emma Wierenga, Peter A. Schrama, Johan W. Gruppen, Harry |
author_facet |
Teuling, Emma Wierenga, Peter A. Schrama, Johan W. Gruppen, Harry |
author_sort |
Teuling, Emma |
title |
Comparison of Protein Extracts from Various Unicellular Green Sources |
title_short |
Comparison of Protein Extracts from Various Unicellular Green Sources |
title_full |
Comparison of Protein Extracts from Various Unicellular Green Sources |
title_fullStr |
Comparison of Protein Extracts from Various Unicellular Green Sources |
title_full_unstemmed |
Comparison of Protein Extracts from Various Unicellular Green Sources |
title_sort |
comparison of protein extracts from various unicellular green sources |
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https://research.wur.nl/en/publications/comparison-of-protein-extracts-from-various-unicellular-green-sou |
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AT teulingemma comparisonofproteinextractsfromvariousunicellulargreensources AT wierengapetera comparisonofproteinextractsfromvariousunicellulargreensources AT schramajohanw comparisonofproteinextractsfromvariousunicellulargreensources AT gruppenharry comparisonofproteinextractsfromvariousunicellulargreensources |
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