Membrane lipids and soluble sugars dynamics of the alkaliphilic fungus Sodiomyces tronii in response to ambient pH
Alkaliphily, the ability of an organism to thrive optimally at high ambient pH, has been well-documented in several lineages: archaea, bacteria and fungi. The molecular mechanics of such adaptation has been extensively addressed in alkaliphilic bacteria and alkalitolerant fungi. In this study, we consider an additional property that may have enabled fungi to prosper at alkaline pH: altered contents of membrane lipids and cytoprotectant molecules. In the alkaliphilic Sodiomyces tronii, we showed that at its optimal growth pH 9.2, the fungus accumulates abundant cytosolic trehalose (4–10% dry weight) and phosphatidic acids in the membrane lipids, properties not normally observed in neutrophilic species. At a very high pH 10.2, the major carbohydrate, glucose, was rapidly substituted by mannitol and arabitol. Conversely, lowering the pH to 5.4–7.0 had major implications both on the content of carbohydrates and membrane lipids. It was shown that trehalose dominated at pH 5.4. Fractions of sphingolipids and sterols of plasma membranes rapidly elevated possibly indicating the formation of membrane structures called rafts. Overall, our results reveals complex dynamics of the contents of membrane lipids and cytoplasmic sugars in alkaliphilic S. tronii, suggesting their adaptive functionality against pH stress.
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Format: | Article/Letter to editor biblioteca |
Language: | English |
Subjects: | Alkaliphilic fungi, Mannitol, Membrane lipids, Sodiomyces tronii, Soluble carbohydrates, Trehalose, |
Online Access: | https://research.wur.nl/en/publications/membrane-lipids-and-soluble-sugars-dynamics-of-the-alkaliphilic-f |
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dig-wur-nl-wurpubs-5211892024-09-23 Bondarenko, Sofiya A. Ianutsevich, Elena A. Danilova, Olga A. Grum-Grzhimaylo, Alexey A. Kotlova, Ekaterina R. Kamzolkina, Olga V. Bilanenko, Elena N. Tereshina, Vera M. Article/Letter to editor Extremophiles 21 (2017) 4 ISSN: 1431-0651 Membrane lipids and soluble sugars dynamics of the alkaliphilic fungus Sodiomyces tronii in response to ambient pH 2017 Alkaliphily, the ability of an organism to thrive optimally at high ambient pH, has been well-documented in several lineages: archaea, bacteria and fungi. The molecular mechanics of such adaptation has been extensively addressed in alkaliphilic bacteria and alkalitolerant fungi. In this study, we consider an additional property that may have enabled fungi to prosper at alkaline pH: altered contents of membrane lipids and cytoprotectant molecules. In the alkaliphilic Sodiomyces tronii, we showed that at its optimal growth pH 9.2, the fungus accumulates abundant cytosolic trehalose (4–10% dry weight) and phosphatidic acids in the membrane lipids, properties not normally observed in neutrophilic species. At a very high pH 10.2, the major carbohydrate, glucose, was rapidly substituted by mannitol and arabitol. Conversely, lowering the pH to 5.4–7.0 had major implications both on the content of carbohydrates and membrane lipids. It was shown that trehalose dominated at pH 5.4. Fractions of sphingolipids and sterols of plasma membranes rapidly elevated possibly indicating the formation of membrane structures called rafts. Overall, our results reveals complex dynamics of the contents of membrane lipids and cytoplasmic sugars in alkaliphilic S. tronii, suggesting their adaptive functionality against pH stress. en application/pdf https://research.wur.nl/en/publications/membrane-lipids-and-soluble-sugars-dynamics-of-the-alkaliphilic-f 10.1007/s00792-017-0940-4 https://edepot.wur.nl/416205 Alkaliphilic fungi Mannitol Membrane lipids Sodiomyces tronii Soluble carbohydrates Trehalose Wageningen University & Research |
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Alkaliphilic fungi Mannitol Membrane lipids Sodiomyces tronii Soluble carbohydrates Trehalose Alkaliphilic fungi Mannitol Membrane lipids Sodiomyces tronii Soluble carbohydrates Trehalose |
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Alkaliphilic fungi Mannitol Membrane lipids Sodiomyces tronii Soluble carbohydrates Trehalose Alkaliphilic fungi Mannitol Membrane lipids Sodiomyces tronii Soluble carbohydrates Trehalose Bondarenko, Sofiya A. Ianutsevich, Elena A. Danilova, Olga A. Grum-Grzhimaylo, Alexey A. Kotlova, Ekaterina R. Kamzolkina, Olga V. Bilanenko, Elena N. Tereshina, Vera M. Membrane lipids and soluble sugars dynamics of the alkaliphilic fungus Sodiomyces tronii in response to ambient pH |
description |
Alkaliphily, the ability of an organism to thrive optimally at high ambient pH, has been well-documented in several lineages: archaea, bacteria and fungi. The molecular mechanics of such adaptation has been extensively addressed in alkaliphilic bacteria and alkalitolerant fungi. In this study, we consider an additional property that may have enabled fungi to prosper at alkaline pH: altered contents of membrane lipids and cytoprotectant molecules. In the alkaliphilic Sodiomyces tronii, we showed that at its optimal growth pH 9.2, the fungus accumulates abundant cytosolic trehalose (4–10% dry weight) and phosphatidic acids in the membrane lipids, properties not normally observed in neutrophilic species. At a very high pH 10.2, the major carbohydrate, glucose, was rapidly substituted by mannitol and arabitol. Conversely, lowering the pH to 5.4–7.0 had major implications both on the content of carbohydrates and membrane lipids. It was shown that trehalose dominated at pH 5.4. Fractions of sphingolipids and sterols of plasma membranes rapidly elevated possibly indicating the formation of membrane structures called rafts. Overall, our results reveals complex dynamics of the contents of membrane lipids and cytoplasmic sugars in alkaliphilic S. tronii, suggesting their adaptive functionality against pH stress. |
format |
Article/Letter to editor |
topic_facet |
Alkaliphilic fungi Mannitol Membrane lipids Sodiomyces tronii Soluble carbohydrates Trehalose |
author |
Bondarenko, Sofiya A. Ianutsevich, Elena A. Danilova, Olga A. Grum-Grzhimaylo, Alexey A. Kotlova, Ekaterina R. Kamzolkina, Olga V. Bilanenko, Elena N. Tereshina, Vera M. |
author_facet |
Bondarenko, Sofiya A. Ianutsevich, Elena A. Danilova, Olga A. Grum-Grzhimaylo, Alexey A. Kotlova, Ekaterina R. Kamzolkina, Olga V. Bilanenko, Elena N. Tereshina, Vera M. |
author_sort |
Bondarenko, Sofiya A. |
title |
Membrane lipids and soluble sugars dynamics of the alkaliphilic fungus Sodiomyces tronii in response to ambient pH |
title_short |
Membrane lipids and soluble sugars dynamics of the alkaliphilic fungus Sodiomyces tronii in response to ambient pH |
title_full |
Membrane lipids and soluble sugars dynamics of the alkaliphilic fungus Sodiomyces tronii in response to ambient pH |
title_fullStr |
Membrane lipids and soluble sugars dynamics of the alkaliphilic fungus Sodiomyces tronii in response to ambient pH |
title_full_unstemmed |
Membrane lipids and soluble sugars dynamics of the alkaliphilic fungus Sodiomyces tronii in response to ambient pH |
title_sort |
membrane lipids and soluble sugars dynamics of the alkaliphilic fungus sodiomyces tronii in response to ambient ph |
url |
https://research.wur.nl/en/publications/membrane-lipids-and-soluble-sugars-dynamics-of-the-alkaliphilic-f |
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