Glass-formingproperty of hydroxyectoine is the cause of its superior function as a desiccation protectant
We were able to demonstrate that hydroxyectoine, in contrast to ectoine, is a good glass-forming compound. Fourier transform infrared and spin label electron spin resonance studies of dry ectoine and hydroxyectoine have shown that the superior glass-forming properties of hydroxyectoine result from stronger intermolecular H-bonds with the OH group of hydroxyectoine. Spin probe experiments have also shown that better molecular immobilization in dry hydroxyectoine provides better redox stability of the molecules embedded in this dry matrix. With a glass transition temperature of 87°C (vs. 47°C for ectoine) hydroxyectoine displays remarkable desiccation protection properties, on a par with sucrose and trehalose. This explains its accumulation in response to increased salinity and elevated temperature by halophiles such as Halomonas elongata and its successful application in “anhydrobiotic engineering” of both enzymes and whole cells.
Main Authors: | , , , , |
---|---|
Format: | Article/Letter to editor biblioteca |
Language: | English |
Subjects: | alternating copolymers, chromohalobacter-salexigens, compatible solute hydroxyectoine, ectoine hydroxylase, escherichia-coli, halophilic eubacteria, pseudomonas-putida, sugar glasses, transition temperatures, trehalose dihydrate, |
Online Access: | https://research.wur.nl/en/publications/glass-formingproperty-of-hydroxyectoine-is-the-cause-of-its-super |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-wur-nl-wurpubs-481015 |
---|---|
record_format |
koha |
spelling |
dig-wur-nl-wurpubs-4810152024-09-23 Tanne, C. Golovina, E.A. Hoekstra, F.A. Meffert, A. Galinski, E.A. Article/Letter to editor Frontiers in Microbiology 5 (2014) ISSN: 1664-302X Glass-formingproperty of hydroxyectoine is the cause of its superior function as a desiccation protectant 2014 We were able to demonstrate that hydroxyectoine, in contrast to ectoine, is a good glass-forming compound. Fourier transform infrared and spin label electron spin resonance studies of dry ectoine and hydroxyectoine have shown that the superior glass-forming properties of hydroxyectoine result from stronger intermolecular H-bonds with the OH group of hydroxyectoine. Spin probe experiments have also shown that better molecular immobilization in dry hydroxyectoine provides better redox stability of the molecules embedded in this dry matrix. With a glass transition temperature of 87°C (vs. 47°C for ectoine) hydroxyectoine displays remarkable desiccation protection properties, on a par with sucrose and trehalose. This explains its accumulation in response to increased salinity and elevated temperature by halophiles such as Halomonas elongata and its successful application in “anhydrobiotic engineering” of both enzymes and whole cells. en application/pdf https://research.wur.nl/en/publications/glass-formingproperty-of-hydroxyectoine-is-the-cause-of-its-super 10.3389/fmicb.2014.00150 https://edepot.wur.nl/327865 alternating copolymers chromohalobacter-salexigens compatible solute hydroxyectoine ectoine hydroxylase escherichia-coli halophilic eubacteria pseudomonas-putida sugar glasses transition temperatures trehalose dihydrate Wageningen University & Research |
institution |
WUR NL |
collection |
DSpace |
country |
Países bajos |
countrycode |
NL |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-wur-nl |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
WUR Library Netherlands |
language |
English |
topic |
alternating copolymers chromohalobacter-salexigens compatible solute hydroxyectoine ectoine hydroxylase escherichia-coli halophilic eubacteria pseudomonas-putida sugar glasses transition temperatures trehalose dihydrate alternating copolymers chromohalobacter-salexigens compatible solute hydroxyectoine ectoine hydroxylase escherichia-coli halophilic eubacteria pseudomonas-putida sugar glasses transition temperatures trehalose dihydrate |
spellingShingle |
alternating copolymers chromohalobacter-salexigens compatible solute hydroxyectoine ectoine hydroxylase escherichia-coli halophilic eubacteria pseudomonas-putida sugar glasses transition temperatures trehalose dihydrate alternating copolymers chromohalobacter-salexigens compatible solute hydroxyectoine ectoine hydroxylase escherichia-coli halophilic eubacteria pseudomonas-putida sugar glasses transition temperatures trehalose dihydrate Tanne, C. Golovina, E.A. Hoekstra, F.A. Meffert, A. Galinski, E.A. Glass-formingproperty of hydroxyectoine is the cause of its superior function as a desiccation protectant |
description |
We were able to demonstrate that hydroxyectoine, in contrast to ectoine, is a good glass-forming compound. Fourier transform infrared and spin label electron spin resonance studies of dry ectoine and hydroxyectoine have shown that the superior glass-forming properties of hydroxyectoine result from stronger intermolecular H-bonds with the OH group of hydroxyectoine. Spin probe experiments have also shown that better molecular immobilization in dry hydroxyectoine provides better redox stability of the molecules embedded in this dry matrix. With a glass transition temperature of 87°C (vs. 47°C for ectoine) hydroxyectoine displays remarkable desiccation protection properties, on a par with sucrose and trehalose. This explains its accumulation in response to increased salinity and elevated temperature by halophiles such as Halomonas elongata and its successful application in “anhydrobiotic engineering” of both enzymes and whole cells. |
format |
Article/Letter to editor |
topic_facet |
alternating copolymers chromohalobacter-salexigens compatible solute hydroxyectoine ectoine hydroxylase escherichia-coli halophilic eubacteria pseudomonas-putida sugar glasses transition temperatures trehalose dihydrate |
author |
Tanne, C. Golovina, E.A. Hoekstra, F.A. Meffert, A. Galinski, E.A. |
author_facet |
Tanne, C. Golovina, E.A. Hoekstra, F.A. Meffert, A. Galinski, E.A. |
author_sort |
Tanne, C. |
title |
Glass-formingproperty of hydroxyectoine is the cause of its superior function as a desiccation protectant |
title_short |
Glass-formingproperty of hydroxyectoine is the cause of its superior function as a desiccation protectant |
title_full |
Glass-formingproperty of hydroxyectoine is the cause of its superior function as a desiccation protectant |
title_fullStr |
Glass-formingproperty of hydroxyectoine is the cause of its superior function as a desiccation protectant |
title_full_unstemmed |
Glass-formingproperty of hydroxyectoine is the cause of its superior function as a desiccation protectant |
title_sort |
glass-formingproperty of hydroxyectoine is the cause of its superior function as a desiccation protectant |
url |
https://research.wur.nl/en/publications/glass-formingproperty-of-hydroxyectoine-is-the-cause-of-its-super |
work_keys_str_mv |
AT tannec glassformingpropertyofhydroxyectoineisthecauseofitssuperiorfunctionasadesiccationprotectant AT golovinaea glassformingpropertyofhydroxyectoineisthecauseofitssuperiorfunctionasadesiccationprotectant AT hoekstrafa glassformingpropertyofhydroxyectoineisthecauseofitssuperiorfunctionasadesiccationprotectant AT mefferta glassformingpropertyofhydroxyectoineisthecauseofitssuperiorfunctionasadesiccationprotectant AT galinskiea glassformingpropertyofhydroxyectoineisthecauseofitssuperiorfunctionasadesiccationprotectant |
_version_ |
1813201453970358272 |