Protoanemonin: a natural quorum sensing inhibitor that selectively activates iron starvation response
Many Gram-negative bacteria employ cell-to-cell communication mediated by N-acyl homoserine lactones (quorum sensing) to control expression of a wide range of genes including, but not limited to, genes encoding virulence factors. Outside the laboratory, the bacteria live in complex communities where signals may be perceived across species. We here present a newly found natural quorum sensing inhibitor, produced by the pseudomonads Pseudomonas sp. B13 and Pseudomonas reinekei MT1 as a blind end in the biodegradation of organochloride xenobiotics, which inhibits quorum sensing in P. aeruginosa in naturally occurring concentrations. This catabolite, 4-methylenebut-2-en-4-olide, also known as protoanemonin, has been reported to possess antibacterial properties, but seems to have dual functions. Using transcriptomics and proteomics, we found that protoanemonin significantly reduced expression of genes and secretion of proteins known to be under control of quorum sensing in P. aeruginosa. Moreover, we found activation of genes and gene products involved in iron starvation response. It is thus likely that inhibition of quorum sensing, as the production of antibiotics, is a phenomenon found in complex bacterial communities
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Language: | English |
Subjects: | attenuation, bacteria, expression, genes, identification, infection, polymorphonuclear leukocytes, pseudomonas-aeruginosa virulence, pyocyanin, to-cell communication, |
Online Access: | https://research.wur.nl/en/publications/protoanemonin-a-natural-quorum-sensing-inhibitor-that-selectively |
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dig-wur-nl-wurpubs-4348322024-08-16 Fazzini, R.A. Skindersoe, M. Bielecki, M. Puchalka, J. Givskov, M. Martins Dos Santos, V.A.P. Article/Letter to editor Environmental Microbiology 15 (2013) 1 ISSN: 1462-2912 Protoanemonin: a natural quorum sensing inhibitor that selectively activates iron starvation response 2013 Many Gram-negative bacteria employ cell-to-cell communication mediated by N-acyl homoserine lactones (quorum sensing) to control expression of a wide range of genes including, but not limited to, genes encoding virulence factors. Outside the laboratory, the bacteria live in complex communities where signals may be perceived across species. We here present a newly found natural quorum sensing inhibitor, produced by the pseudomonads Pseudomonas sp. B13 and Pseudomonas reinekei MT1 as a blind end in the biodegradation of organochloride xenobiotics, which inhibits quorum sensing in P. aeruginosa in naturally occurring concentrations. This catabolite, 4-methylenebut-2-en-4-olide, also known as protoanemonin, has been reported to possess antibacterial properties, but seems to have dual functions. Using transcriptomics and proteomics, we found that protoanemonin significantly reduced expression of genes and secretion of proteins known to be under control of quorum sensing in P. aeruginosa. Moreover, we found activation of genes and gene products involved in iron starvation response. It is thus likely that inhibition of quorum sensing, as the production of antibiotics, is a phenomenon found in complex bacterial communities en application/pdf https://research.wur.nl/en/publications/protoanemonin-a-natural-quorum-sensing-inhibitor-that-selectively 10.1111/j.1462-2920.2012.02792.x https://edepot.wur.nl/246228 attenuation bacteria expression genes identification infection polymorphonuclear leukocytes pseudomonas-aeruginosa virulence pyocyanin to-cell communication Wageningen University & Research |
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attenuation bacteria expression genes identification infection polymorphonuclear leukocytes pseudomonas-aeruginosa virulence pyocyanin to-cell communication attenuation bacteria expression genes identification infection polymorphonuclear leukocytes pseudomonas-aeruginosa virulence pyocyanin to-cell communication |
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attenuation bacteria expression genes identification infection polymorphonuclear leukocytes pseudomonas-aeruginosa virulence pyocyanin to-cell communication attenuation bacteria expression genes identification infection polymorphonuclear leukocytes pseudomonas-aeruginosa virulence pyocyanin to-cell communication Fazzini, R.A. Skindersoe, M. Bielecki, M. Puchalka, J. Givskov, M. Martins Dos Santos, V.A.P. Protoanemonin: a natural quorum sensing inhibitor that selectively activates iron starvation response |
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Many Gram-negative bacteria employ cell-to-cell communication mediated by N-acyl homoserine lactones (quorum sensing) to control expression of a wide range of genes including, but not limited to, genes encoding virulence factors. Outside the laboratory, the bacteria live in complex communities where signals may be perceived across species. We here present a newly found natural quorum sensing inhibitor, produced by the pseudomonads Pseudomonas sp. B13 and Pseudomonas reinekei MT1 as a blind end in the biodegradation of organochloride xenobiotics, which inhibits quorum sensing in P. aeruginosa in naturally occurring concentrations. This catabolite, 4-methylenebut-2-en-4-olide, also known as protoanemonin, has been reported to possess antibacterial properties, but seems to have dual functions. Using transcriptomics and proteomics, we found that protoanemonin significantly reduced expression of genes and secretion of proteins known to be under control of quorum sensing in P. aeruginosa. Moreover, we found activation of genes and gene products involved in iron starvation response. It is thus likely that inhibition of quorum sensing, as the production of antibiotics, is a phenomenon found in complex bacterial communities |
format |
Article/Letter to editor |
topic_facet |
attenuation bacteria expression genes identification infection polymorphonuclear leukocytes pseudomonas-aeruginosa virulence pyocyanin to-cell communication |
author |
Fazzini, R.A. Skindersoe, M. Bielecki, M. Puchalka, J. Givskov, M. Martins Dos Santos, V.A.P. |
author_facet |
Fazzini, R.A. Skindersoe, M. Bielecki, M. Puchalka, J. Givskov, M. Martins Dos Santos, V.A.P. |
author_sort |
Fazzini, R.A. |
title |
Protoanemonin: a natural quorum sensing inhibitor that selectively activates iron starvation response |
title_short |
Protoanemonin: a natural quorum sensing inhibitor that selectively activates iron starvation response |
title_full |
Protoanemonin: a natural quorum sensing inhibitor that selectively activates iron starvation response |
title_fullStr |
Protoanemonin: a natural quorum sensing inhibitor that selectively activates iron starvation response |
title_full_unstemmed |
Protoanemonin: a natural quorum sensing inhibitor that selectively activates iron starvation response |
title_sort |
protoanemonin: a natural quorum sensing inhibitor that selectively activates iron starvation response |
url |
https://research.wur.nl/en/publications/protoanemonin-a-natural-quorum-sensing-inhibitor-that-selectively |
work_keys_str_mv |
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_version_ |
1813022772134150144 |