Aiming for the complete utilization of sugar-beet pulp: Examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion

Background - Biomass use for the production of bioethanol or platform chemicals requires efficient breakdown of biomass to fermentable monosaccharides. Lignocellulosic feedstocks often require physicochemical pretreatment before enzymatic hydrolysis can begin. The optimal pretreatment can be different for different feedstocks, and should not lead to biomass destruction or formation of toxic products. Methods - We examined the influence of six mild sulfuric acid or water pretreatments at different temperatures on the enzymatic degradability of sugar-beet pulp (SBP). Results - We found that optimal pretreatment at 140°C of 15 minutes in water was able to solubilize 60% w/w of the total carbohydrates present, mainly pectins. More severe treatments led to the destruction of the solubilized sugars, and the subsequent production of the sugar-degradation products furfural, hydroxymethylfurfural, acetic acid and formic acid. The pretreated samples were successfully degraded enzymatically with an experimental cellulase preparation. Conclusions - In this study, we found that pretreatment of SBP greatly facilitated the subsequent enzymatic degradation within economically feasible time ranges and enzyme levels. In addition, pretreatment of SBP can be useful to fractionate functional ingredients such as arabinans and pectins from cellulose. We found that the optimal combined severity factor to enhance the enzymatic degradation of SBP was between log R'0 = -2.0 and log R'0 = -1.5. The optimal pretreatment and enzyme treatment solubilized up to 80% of all sugars present in the SBP, including =90% of the cellulose.

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Main Authors: Kuhnel, S., Schols, H.A., Gruppen, H.
Format: Article/Letter to editor biblioteca
Language:English
Subjects:cell-wall, cellulose, degradation, fermentation, hydrolysis, inhibition, lignocellulosics, pectins, severity parameter, side-chains,
Online Access:https://research.wur.nl/en/publications/aiming-for-the-complete-utilization-of-sugar-beet-pulp-examinatio
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spelling dig-wur-nl-wurpubs-4180422024-06-25 Kuhnel, S. Schols, H.A. Gruppen, H. Article/Letter to editor Biotechnology for Biofuels 4 (2011) ISSN: 1754-6834 Aiming for the complete utilization of sugar-beet pulp: Examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion 2011 Background - Biomass use for the production of bioethanol or platform chemicals requires efficient breakdown of biomass to fermentable monosaccharides. Lignocellulosic feedstocks often require physicochemical pretreatment before enzymatic hydrolysis can begin. The optimal pretreatment can be different for different feedstocks, and should not lead to biomass destruction or formation of toxic products. Methods - We examined the influence of six mild sulfuric acid or water pretreatments at different temperatures on the enzymatic degradability of sugar-beet pulp (SBP). Results - We found that optimal pretreatment at 140°C of 15 minutes in water was able to solubilize 60% w/w of the total carbohydrates present, mainly pectins. More severe treatments led to the destruction of the solubilized sugars, and the subsequent production of the sugar-degradation products furfural, hydroxymethylfurfural, acetic acid and formic acid. The pretreated samples were successfully degraded enzymatically with an experimental cellulase preparation. Conclusions - In this study, we found that pretreatment of SBP greatly facilitated the subsequent enzymatic degradation within economically feasible time ranges and enzyme levels. In addition, pretreatment of SBP can be useful to fractionate functional ingredients such as arabinans and pectins from cellulose. We found that the optimal combined severity factor to enhance the enzymatic degradation of SBP was between log R'0 = -2.0 and log R'0 = -1.5. The optimal pretreatment and enzyme treatment solubilized up to 80% of all sugars present in the SBP, including =90% of the cellulose. en application/pdf https://research.wur.nl/en/publications/aiming-for-the-complete-utilization-of-sugar-beet-pulp-examinatio 10.1186/1754-6834-4-14 https://edepot.wur.nl/192269 cell-wall cellulose degradation fermentation hydrolysis inhibition lignocellulosics pectins severity parameter side-chains Wageningen University & Research
institution WUR NL
collection DSpace
country Países bajos
countrycode NL
component Bibliográfico
access En linea
databasecode dig-wur-nl
tag biblioteca
region Europa del Oeste
libraryname WUR Library Netherlands
language English
topic cell-wall
cellulose
degradation
fermentation
hydrolysis
inhibition
lignocellulosics
pectins
severity parameter
side-chains
cell-wall
cellulose
degradation
fermentation
hydrolysis
inhibition
lignocellulosics
pectins
severity parameter
side-chains
spellingShingle cell-wall
cellulose
degradation
fermentation
hydrolysis
inhibition
lignocellulosics
pectins
severity parameter
side-chains
cell-wall
cellulose
degradation
fermentation
hydrolysis
inhibition
lignocellulosics
pectins
severity parameter
side-chains
Kuhnel, S.
Schols, H.A.
Gruppen, H.
Aiming for the complete utilization of sugar-beet pulp: Examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion
description Background - Biomass use for the production of bioethanol or platform chemicals requires efficient breakdown of biomass to fermentable monosaccharides. Lignocellulosic feedstocks often require physicochemical pretreatment before enzymatic hydrolysis can begin. The optimal pretreatment can be different for different feedstocks, and should not lead to biomass destruction or formation of toxic products. Methods - We examined the influence of six mild sulfuric acid or water pretreatments at different temperatures on the enzymatic degradability of sugar-beet pulp (SBP). Results - We found that optimal pretreatment at 140°C of 15 minutes in water was able to solubilize 60% w/w of the total carbohydrates present, mainly pectins. More severe treatments led to the destruction of the solubilized sugars, and the subsequent production of the sugar-degradation products furfural, hydroxymethylfurfural, acetic acid and formic acid. The pretreated samples were successfully degraded enzymatically with an experimental cellulase preparation. Conclusions - In this study, we found that pretreatment of SBP greatly facilitated the subsequent enzymatic degradation within economically feasible time ranges and enzyme levels. In addition, pretreatment of SBP can be useful to fractionate functional ingredients such as arabinans and pectins from cellulose. We found that the optimal combined severity factor to enhance the enzymatic degradation of SBP was between log R'0 = -2.0 and log R'0 = -1.5. The optimal pretreatment and enzyme treatment solubilized up to 80% of all sugars present in the SBP, including =90% of the cellulose.
format Article/Letter to editor
topic_facet cell-wall
cellulose
degradation
fermentation
hydrolysis
inhibition
lignocellulosics
pectins
severity parameter
side-chains
author Kuhnel, S.
Schols, H.A.
Gruppen, H.
author_facet Kuhnel, S.
Schols, H.A.
Gruppen, H.
author_sort Kuhnel, S.
title Aiming for the complete utilization of sugar-beet pulp: Examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion
title_short Aiming for the complete utilization of sugar-beet pulp: Examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion
title_full Aiming for the complete utilization of sugar-beet pulp: Examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion
title_fullStr Aiming for the complete utilization of sugar-beet pulp: Examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion
title_full_unstemmed Aiming for the complete utilization of sugar-beet pulp: Examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion
title_sort aiming for the complete utilization of sugar-beet pulp: examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion
url https://research.wur.nl/en/publications/aiming-for-the-complete-utilization-of-sugar-beet-pulp-examinatio
work_keys_str_mv AT kuhnels aimingforthecompleteutilizationofsugarbeetpulpexaminationoftheeffectsofmildacidandhydrothermalpretreatmentfollowedbyenzymaticdigestion
AT scholsha aimingforthecompleteutilizationofsugarbeetpulpexaminationoftheeffectsofmildacidandhydrothermalpretreatmentfollowedbyenzymaticdigestion
AT gruppenh aimingforthecompleteutilizationofsugarbeetpulpexaminationoftheeffectsofmildacidandhydrothermalpretreatmentfollowedbyenzymaticdigestion
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