Efficient sweet pepper transformation mediated by the BABY BOOM transcription factor
Pepper (Capsicum L.) is a nutritionally and economically important crop that is cultivated throughout the world as a vegetable, condiment, and food additive. Genetic transformation using Agrobacterium tumefaciens (agrobacterium) is a powerful biotechnology tool that could be used in pepper to develop community-based functional genomics resources and to introduce important agronomic traits. However, pepper is considered to be highly recalcitrant for agrobacterium-mediated transformation, and current transformation protocols are either inefficient, cumbersome or highly genotype dependent. The main bottleneck in pepper transformation is the inability to generate cells that are competent for both regeneration and transformation. Here, we report that ectopic expression of the Brassica napus BABY BOOM AP2/ERF transcription factor overcomes this bottleneck and can be used to efficiently regenerate transgenic plants from otherwise recalcitrant sweet pepper (C. annuum) varieties. Transient activation of BABY BOOM in the progeny plants induced prolific cell regeneration and was used to produce a large number of somatic embryos that could be converted readily to seedlings. The data highlight the utility of combining biotechnology and classical plant tissue culture approaches to develop an efficient transformation and regeneration system for a highly recalcitrant vegetable crop
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Format: | Article/Letter to editor biblioteca |
Language: | English |
Subjects: | agrobacterium-tumefaciens, arabidopsis, capsicum-annuum l., chinense jacq., direct somatic embryogenesis, genetic-transformation, growth, in-vitro regeneration, plant-regeneration, shoot, |
Online Access: | https://research.wur.nl/en/publications/efficient-sweet-pepper-transformation-mediated-by-the-baby-boom-t |
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dig-wur-nl-wurpubs-4049372024-09-23 Heidmann, I. de Lange, B. Lambalk, J. Angenent, G.C. Boutilier, K. Article/Letter to editor Plant Cell Reports (2011) ISSN: 0721-7714 Efficient sweet pepper transformation mediated by the BABY BOOM transcription factor 2011 Pepper (Capsicum L.) is a nutritionally and economically important crop that is cultivated throughout the world as a vegetable, condiment, and food additive. Genetic transformation using Agrobacterium tumefaciens (agrobacterium) is a powerful biotechnology tool that could be used in pepper to develop community-based functional genomics resources and to introduce important agronomic traits. However, pepper is considered to be highly recalcitrant for agrobacterium-mediated transformation, and current transformation protocols are either inefficient, cumbersome or highly genotype dependent. The main bottleneck in pepper transformation is the inability to generate cells that are competent for both regeneration and transformation. Here, we report that ectopic expression of the Brassica napus BABY BOOM AP2/ERF transcription factor overcomes this bottleneck and can be used to efficiently regenerate transgenic plants from otherwise recalcitrant sweet pepper (C. annuum) varieties. Transient activation of BABY BOOM in the progeny plants induced prolific cell regeneration and was used to produce a large number of somatic embryos that could be converted readily to seedlings. The data highlight the utility of combining biotechnology and classical plant tissue culture approaches to develop an efficient transformation and regeneration system for a highly recalcitrant vegetable crop en application/pdf https://research.wur.nl/en/publications/efficient-sweet-pepper-transformation-mediated-by-the-baby-boom-t 10.1007/s00299-011-1018-x https://edepot.wur.nl/165209 agrobacterium-tumefaciens arabidopsis capsicum-annuum l. chinense jacq. direct somatic embryogenesis genetic-transformation growth in-vitro regeneration plant-regeneration shoot Wageningen University & Research |
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agrobacterium-tumefaciens arabidopsis capsicum-annuum l. chinense jacq. direct somatic embryogenesis genetic-transformation growth in-vitro regeneration plant-regeneration shoot agrobacterium-tumefaciens arabidopsis capsicum-annuum l. chinense jacq. direct somatic embryogenesis genetic-transformation growth in-vitro regeneration plant-regeneration shoot |
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agrobacterium-tumefaciens arabidopsis capsicum-annuum l. chinense jacq. direct somatic embryogenesis genetic-transformation growth in-vitro regeneration plant-regeneration shoot agrobacterium-tumefaciens arabidopsis capsicum-annuum l. chinense jacq. direct somatic embryogenesis genetic-transformation growth in-vitro regeneration plant-regeneration shoot Heidmann, I. de Lange, B. Lambalk, J. Angenent, G.C. Boutilier, K. Efficient sweet pepper transformation mediated by the BABY BOOM transcription factor |
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Pepper (Capsicum L.) is a nutritionally and economically important crop that is cultivated throughout the world as a vegetable, condiment, and food additive. Genetic transformation using Agrobacterium tumefaciens (agrobacterium) is a powerful biotechnology tool that could be used in pepper to develop community-based functional genomics resources and to introduce important agronomic traits. However, pepper is considered to be highly recalcitrant for agrobacterium-mediated transformation, and current transformation protocols are either inefficient, cumbersome or highly genotype dependent. The main bottleneck in pepper transformation is the inability to generate cells that are competent for both regeneration and transformation. Here, we report that ectopic expression of the Brassica napus BABY BOOM AP2/ERF transcription factor overcomes this bottleneck and can be used to efficiently regenerate transgenic plants from otherwise recalcitrant sweet pepper (C. annuum) varieties. Transient activation of BABY BOOM in the progeny plants induced prolific cell regeneration and was used to produce a large number of somatic embryos that could be converted readily to seedlings. The data highlight the utility of combining biotechnology and classical plant tissue culture approaches to develop an efficient transformation and regeneration system for a highly recalcitrant vegetable crop |
format |
Article/Letter to editor |
topic_facet |
agrobacterium-tumefaciens arabidopsis capsicum-annuum l. chinense jacq. direct somatic embryogenesis genetic-transformation growth in-vitro regeneration plant-regeneration shoot |
author |
Heidmann, I. de Lange, B. Lambalk, J. Angenent, G.C. Boutilier, K. |
author_facet |
Heidmann, I. de Lange, B. Lambalk, J. Angenent, G.C. Boutilier, K. |
author_sort |
Heidmann, I. |
title |
Efficient sweet pepper transformation mediated by the BABY BOOM transcription factor |
title_short |
Efficient sweet pepper transformation mediated by the BABY BOOM transcription factor |
title_full |
Efficient sweet pepper transformation mediated by the BABY BOOM transcription factor |
title_fullStr |
Efficient sweet pepper transformation mediated by the BABY BOOM transcription factor |
title_full_unstemmed |
Efficient sweet pepper transformation mediated by the BABY BOOM transcription factor |
title_sort |
efficient sweet pepper transformation mediated by the baby boom transcription factor |
url |
https://research.wur.nl/en/publications/efficient-sweet-pepper-transformation-mediated-by-the-baby-boom-t |
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