Open reading frame 132 of Helicoverpa armigera nucleopolyhedrovirus encodes a functional per os infectivity factor (PIF-2)
Open reading frame 132 (Ha132) of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) is a homologue of per os infectivity factor 2 (pif-2) of Spodoptera exigua multiple nucleopolyhedrovirus. Sequence analysis indicated that Ha132 encoded a protein of 383 aa with a predicted molecular mass of 44.5 kDa. Alignment of HA132 and its baculovirus homologues revealed that HA132 was highly conserved among baculoviruses, with 14 absolutely conserved cysteine residues. RT-PCR indicated that Ha132 was first transcribed at 24 h post-infection. Western blot analysis showed that a 43 kDa band was detectable in HearNPV-infected HzAM1 cells from 36 h post-infection. Western blots also indicated that HA132 was a component of the occlusion-derived virus, but not of budded virus. Deletion of Ha132 from HearNPV abolished per os infectivity, but had no effect on the infectivity of the budded virus phenotype
Main Authors: | Fang, M., Nie, Y., Wang, Q., Deng, F., Wang, R., Wang, H., Vlak, J.M., Chen, X., Hu, Z.H. |
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Format: | Article/Letter to editor biblioteca |
Language: | English |
Subjects: | autographa-californica nucleopolyhedrovirus, envelope protein p74, heliothis-virescens larvae, multiple nucleopolyhedrovirus, nuclear polyhedrosis-virus, nucleotide-s, occlusion-derived virus, orgyia-pseudotsugata, single-nucleocapsid nucleopolyhedrovirus, |
Online Access: | https://research.wur.nl/en/publications/open-reading-frame-132-of-helicoverpa-armigera-nucleopolyhedrovir |
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