Characterization of a nucleopolyhedrovirus isolated from the laboratory rearing of the beet armyworm Spodoptera Exigua (HBN.) in Poland
A nucleopolyhedrovirus isolated from the beet armyworm Spodoptera exigua (Polish laboratory culture), SeMNPV (P), morphologically similar to the viral bioinsecticide virus Spod-XR, was characterized molecularly and biologically. Phylogenetic analysis based on three conserved baculovirus genes, polh, lef-8 and pif-2, showed the highest homology of SeMNPV (P) to Mamestra brassicae (Mb) MNPV and M. configurata (Maco) MNPV, and much less to SeMNPV (Spod-XR). These findings were confirmed by genomic DNA restriction profile analyses. Bioassays revealed that SeMNPV isolated from the commercial bioinsecticide Spod-XR was themost infectious for S. exigua, while the infectivity of SeMNPV (P) and MbMNPV was significantly lower. These data suggest that SeMNPV (P) is a variant of MbMNPV.
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dig-wur-nl-wurpubs-3458002024-09-23 Jakubowska, A.K. Vlak, J.M. Ziemnicka, J. Article/Letter to editor Journal of Plant Protection Research 45 (2005) 4 ISSN: 1427-4345 Characterization of a nucleopolyhedrovirus isolated from the laboratory rearing of the beet armyworm Spodoptera Exigua (HBN.) in Poland 2005 A nucleopolyhedrovirus isolated from the beet armyworm Spodoptera exigua (Polish laboratory culture), SeMNPV (P), morphologically similar to the viral bioinsecticide virus Spod-XR, was characterized molecularly and biologically. Phylogenetic analysis based on three conserved baculovirus genes, polh, lef-8 and pif-2, showed the highest homology of SeMNPV (P) to Mamestra brassicae (Mb) MNPV and M. configurata (Maco) MNPV, and much less to SeMNPV (Spod-XR). These findings were confirmed by genomic DNA restriction profile analyses. Bioassays revealed that SeMNPV isolated from the commercial bioinsecticide Spod-XR was themost infectious for S. exigua, while the infectivity of SeMNPV (P) and MbMNPV was significantly lower. These data suggest that SeMNPV (P) is a variant of MbMNPV. en application/pdf https://research.wur.nl/en/publications/characterization-of-a-nucleopolyhedrovirus-isolated-from-the-labo https://edepot.wur.nl/29694 Life Science Wageningen University & Research |
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Life Science Life Science Jakubowska, A.K. Vlak, J.M. Ziemnicka, J. Characterization of a nucleopolyhedrovirus isolated from the laboratory rearing of the beet armyworm Spodoptera Exigua (HBN.) in Poland |
| description |
A nucleopolyhedrovirus isolated from the beet armyworm Spodoptera exigua (Polish laboratory culture), SeMNPV (P), morphologically similar to the viral bioinsecticide virus Spod-XR, was characterized molecularly and biologically. Phylogenetic analysis based on three conserved baculovirus genes, polh, lef-8 and pif-2, showed the highest homology of SeMNPV (P) to Mamestra brassicae (Mb) MNPV and M. configurata (Maco) MNPV, and much less to SeMNPV (Spod-XR). These findings were confirmed by genomic DNA restriction profile analyses. Bioassays revealed that SeMNPV isolated from the commercial bioinsecticide Spod-XR was themost infectious for S. exigua, while the infectivity of SeMNPV (P) and MbMNPV was significantly lower. These data suggest that SeMNPV (P) is a variant of MbMNPV. |
| format |
Article/Letter to editor |
| topic_facet |
Life Science |
| author |
Jakubowska, A.K. Vlak, J.M. Ziemnicka, J. |
| author_facet |
Jakubowska, A.K. Vlak, J.M. Ziemnicka, J. |
| author_sort |
Jakubowska, A.K. |
| title |
Characterization of a nucleopolyhedrovirus isolated from the laboratory rearing of the beet armyworm Spodoptera Exigua (HBN.) in Poland |
| title_short |
Characterization of a nucleopolyhedrovirus isolated from the laboratory rearing of the beet armyworm Spodoptera Exigua (HBN.) in Poland |
| title_full |
Characterization of a nucleopolyhedrovirus isolated from the laboratory rearing of the beet armyworm Spodoptera Exigua (HBN.) in Poland |
| title_fullStr |
Characterization of a nucleopolyhedrovirus isolated from the laboratory rearing of the beet armyworm Spodoptera Exigua (HBN.) in Poland |
| title_full_unstemmed |
Characterization of a nucleopolyhedrovirus isolated from the laboratory rearing of the beet armyworm Spodoptera Exigua (HBN.) in Poland |
| title_sort |
characterization of a nucleopolyhedrovirus isolated from the laboratory rearing of the beet armyworm spodoptera exigua (hbn.) in poland |
| url |
https://research.wur.nl/en/publications/characterization-of-a-nucleopolyhedrovirus-isolated-from-the-labo |
| work_keys_str_mv |
AT jakubowskaak characterizationofanucleopolyhedrovirusisolatedfromthelaboratoryrearingofthebeetarmywormspodopteraexiguahbninpoland AT vlakjm characterizationofanucleopolyhedrovirusisolatedfromthelaboratoryrearingofthebeetarmywormspodopteraexiguahbninpoland AT ziemnickaj characterizationofanucleopolyhedrovirusisolatedfromthelaboratoryrearingofthebeetarmywormspodopteraexiguahbninpoland |
| _version_ |
1816163089751474176 |