Overproduction of heterologous mannitol 1-phosphatase : a key factor for engineering mannitol production by Lactococcus lactis

To achieve high mannitol production by Lactococcus lactis, the mannitol 1-phosphatase gene of Eimeria tenella and the mannitol 1-phosphate dehydrogenase gene mtlD of Lactobacillus plantarum were cloned in the nisin-dependent L. lactis NICE overexpression system. As predicted by a kinetic L. lactis glycolysis model, increase in mannitol 1-phosphate dehydrogenase and mannitol 1-phosphatase activities resulted in increased mannitol production. Overexpression of both genes in growing cells resulted in glucose-mannitol conversions of 11, 21, and 27% by the L. lactis parental strain, a strain with reduced phosphofructokinase activity, and a lactate dehydrogenase-deficient strain, respectively. Improved induction conditions and increased substrate concentrations resulted in an even higher glucose-to-mannitol conversion of 50% by the lactate dehydrogenase-deficient L. lactis strain, close to the theoretical mannitol yield of 67%. Moreover, a clear correlation between mannitol 1-phosphatase activity and mannitol production was shown, demonstrating the usefulness of this metabolic engineering approach.

Bibliographic Details

Main Authors:	Wisselink, H.W., Moers, A.P.H.A., Mars, A.E., Hoefnagel, M.H.N., de Vos, W.M., Hugenholtz, J.
Format:	Article/Letter to editor biblioteca
Language:	English
Subjects:	acid bacteria, complete genome sequence, controlled gene-expression, deficient, glucose, lactate-dehydrogenase, lactobacillus-plantarum, leuconostoc, mesenteroides, protects,
Online Access:	https://research.wur.nl/en/publications/overproduction-of-heterologous-mannitol-1-phosphatase-a-key-facto
Tags:	Add Tag No Tags, Be the first to tag this record!