Mapping and characterisation of quantitative trait loci conferring nematode resistance in Solanum spegazzinii
This thesis describes the mapping and characterisation of QTLs, in Solanum spegazzinii, that are involved in resistance to the potato cyst-nematodes Globodera rostochiensis and G. pallida. For this purpose an RFLP linkage map of potato is constructed based on the offspring from non-inbred parents. Phenomena like distorted segregation and reduced recombination that are observed during linkage analysis are discussed. Several QTLs are mapped that are involved in resistance to G.rostochiensis and one major QTL that is involved in resistance to G.pallida. Single point analysis and interval mapping are employed for the localisation of the QTLs. Other quantitative traits like tuber yield and root development are mapped as well. In addition a method is described for the nonradioactive detection of single copy DNA-DNA hybrids.
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Format: | Doctoral thesis biblioteca |
Language: | English |
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Landbouwuniversiteit Wageningen
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Subjects: | chromosome maps, disease resistance, gene mapping, genetic engineering, globodera, heteroderidae, pest resistance, plant breeding, plant pests, plants, potatoes, pratylenchus, recombinant dna, solanum tuberosum, tylenchidae, aardappelen, chromosoomkaarten, genetische modificatie, genkartering, plaagresistentie, planten, plantenplagen, plantenveredeling, ziekteresistentie, |
Online Access: | https://research.wur.nl/en/publications/mapping-and-characterisation-of-quantitative-trait-loci-conferrin |
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dig-wur-nl-wurpubs-312202024-09-23 Kreike, C.M. Heyting, C. Stiekema, W.J. Doctoral thesis Mapping and characterisation of quantitative trait loci conferring nematode resistance in Solanum spegazzinii 1995 This thesis describes the mapping and characterisation of QTLs, in Solanum spegazzinii, that are involved in resistance to the potato cyst-nematodes Globodera rostochiensis and G. pallida. For this purpose an RFLP linkage map of potato is constructed based on the offspring from non-inbred parents. Phenomena like distorted segregation and reduced recombination that are observed during linkage analysis are discussed. Several QTLs are mapped that are involved in resistance to G.rostochiensis and one major QTL that is involved in resistance to G.pallida. Single point analysis and interval mapping are employed for the localisation of the QTLs. Other quantitative traits like tuber yield and root development are mapped as well. In addition a method is described for the nonradioactive detection of single copy DNA-DNA hybrids. en Landbouwuniversiteit Wageningen application/pdf https://research.wur.nl/en/publications/mapping-and-characterisation-of-quantitative-trait-loci-conferrin https://edepot.wur.nl/205445 chromosome maps disease resistance gene mapping genetic engineering globodera heteroderidae pest resistance plant breeding plant pests plants potatoes pratylenchus recombinant dna solanum tuberosum tylenchidae aardappelen chromosoomkaarten genetische modificatie genkartering globodera heteroderidae plaagresistentie planten plantenplagen plantenveredeling pratylenchus recombinant dna solanum tuberosum tylenchidae ziekteresistentie Wageningen University & Research |
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chromosome maps disease resistance gene mapping genetic engineering globodera heteroderidae pest resistance plant breeding plant pests plants potatoes pratylenchus recombinant dna solanum tuberosum tylenchidae aardappelen chromosoomkaarten genetische modificatie genkartering globodera heteroderidae plaagresistentie planten plantenplagen plantenveredeling pratylenchus recombinant dna solanum tuberosum tylenchidae ziekteresistentie chromosome maps disease resistance gene mapping genetic engineering globodera heteroderidae pest resistance plant breeding plant pests plants potatoes pratylenchus recombinant dna solanum tuberosum tylenchidae aardappelen chromosoomkaarten genetische modificatie genkartering globodera heteroderidae plaagresistentie planten plantenplagen plantenveredeling pratylenchus recombinant dna solanum tuberosum tylenchidae ziekteresistentie |
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chromosome maps disease resistance gene mapping genetic engineering globodera heteroderidae pest resistance plant breeding plant pests plants potatoes pratylenchus recombinant dna solanum tuberosum tylenchidae aardappelen chromosoomkaarten genetische modificatie genkartering globodera heteroderidae plaagresistentie planten plantenplagen plantenveredeling pratylenchus recombinant dna solanum tuberosum tylenchidae ziekteresistentie chromosome maps disease resistance gene mapping genetic engineering globodera heteroderidae pest resistance plant breeding plant pests plants potatoes pratylenchus recombinant dna solanum tuberosum tylenchidae aardappelen chromosoomkaarten genetische modificatie genkartering globodera heteroderidae plaagresistentie planten plantenplagen plantenveredeling pratylenchus recombinant dna solanum tuberosum tylenchidae ziekteresistentie Kreike, C.M. Mapping and characterisation of quantitative trait loci conferring nematode resistance in Solanum spegazzinii |
description |
This thesis describes the mapping and characterisation of QTLs, in Solanum spegazzinii, that are involved in resistance to the potato cyst-nematodes Globodera rostochiensis and G. pallida. For this purpose an RFLP linkage map of potato is constructed based on the offspring from non-inbred parents. Phenomena like distorted segregation and reduced recombination that are observed during linkage analysis are discussed. Several QTLs are mapped that are involved in resistance to G.rostochiensis and one major QTL that is involved in resistance to G.pallida. Single point analysis and interval mapping are employed for the localisation of the QTLs. Other quantitative traits like tuber yield and root development are mapped as well. In addition a method is described for the nonradioactive detection of single copy DNA-DNA hybrids. |
author2 |
Heyting, C. |
author_facet |
Heyting, C. Kreike, C.M. |
format |
Doctoral thesis |
topic_facet |
chromosome maps disease resistance gene mapping genetic engineering globodera heteroderidae pest resistance plant breeding plant pests plants potatoes pratylenchus recombinant dna solanum tuberosum tylenchidae aardappelen chromosoomkaarten genetische modificatie genkartering globodera heteroderidae plaagresistentie planten plantenplagen plantenveredeling pratylenchus recombinant dna solanum tuberosum tylenchidae ziekteresistentie |
author |
Kreike, C.M. |
author_sort |
Kreike, C.M. |
title |
Mapping and characterisation of quantitative trait loci conferring nematode resistance in Solanum spegazzinii |
title_short |
Mapping and characterisation of quantitative trait loci conferring nematode resistance in Solanum spegazzinii |
title_full |
Mapping and characterisation of quantitative trait loci conferring nematode resistance in Solanum spegazzinii |
title_fullStr |
Mapping and characterisation of quantitative trait loci conferring nematode resistance in Solanum spegazzinii |
title_full_unstemmed |
Mapping and characterisation of quantitative trait loci conferring nematode resistance in Solanum spegazzinii |
title_sort |
mapping and characterisation of quantitative trait loci conferring nematode resistance in solanum spegazzinii |
publisher |
Landbouwuniversiteit Wageningen |
url |
https://research.wur.nl/en/publications/mapping-and-characterisation-of-quantitative-trait-loci-conferrin |
work_keys_str_mv |
AT kreikecm mappingandcharacterisationofquantitativetraitlociconferringnematoderesistanceinsolanumspegazzinii |
_version_ |
1813211428760322048 |