Molecular and Biochemical Characterization of a Distinct Type of Fructose-1,6- Bisphosphatase from Pyrococcus furiosus
The Pyrococcus furiosus fbpA gene was cloned and expressed in Escherichia coli, and the fructose-1,6-bisphosphatase produced was subsequently purified and characterized. The dimeric enzyme showed a preference for fructose-1,6-bisphosphate, with a Km of 0.32 mM and a Vmax of 12.2 U/mg. The P. furiosus fructose-1,6-bisphosphatase was strongly inhibited by Li (50␒nhibitory concentration, 1 mM). Based on the presence of conserved sequence motifs and the substrate specificity of the P. furiosus fructose-1,6-bisphosphatase, we propose that this enzyme belongs to a new family, class IV fructose-1,6-bisphosphatase.
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Main Authors: | Verhees, C.H., Akkerboom, J., Schiltz, J., de Vos, W.M., van der Oost, J. |
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Format: | Article/Letter to editor biblioteca |
Language: | English |
Subjects: | Life Science, |
Online Access: | https://research.wur.nl/en/publications/molecular-and-biochemical-characterization-of-a-distinct-type-of- |
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