Sex differences in active DNA demethylation machinery during the critical period of brain masculinization
Fil: Bigarani, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | conferenceObject biblioteca |
| Language: | eng |
| Published: |
2022
|
| Subjects: | DNA demethylation, sexual differentiation, epigenetics, |
| Online Access: | http://hdl.handle.net/11086/549574 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-unc-ar-11086-549574 |
|---|---|
| record_format |
koha |
| institution |
UNC AR |
| collection |
DSpace |
| country |
Argentina |
| countrycode |
AR |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-unc-ar |
| tag |
biblioteca |
| region |
America del Sur |
| libraryname |
Biblioteca 'Ing. Agrónomo Moisés Farber' de la Facultad de Ciencias Agropecuarias |
| language |
eng |
| topic |
DNA demethylation sexual differentiation epigenetics DNA demethylation sexual differentiation epigenetics |
| spellingShingle |
DNA demethylation sexual differentiation epigenetics DNA demethylation sexual differentiation epigenetics Bigarani, Rocío Villarreal, Macarena Cambiasso, María Julia Cisternas, Carla Daniela Sex differences in active DNA demethylation machinery during the critical period of brain masculinization |
| description |
Fil: Bigarani, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. |
| format |
conferenceObject |
| topic_facet |
DNA demethylation sexual differentiation epigenetics |
| author |
Bigarani, Rocío Villarreal, Macarena Cambiasso, María Julia Cisternas, Carla Daniela |
| author_facet |
Bigarani, Rocío Villarreal, Macarena Cambiasso, María Julia Cisternas, Carla Daniela |
| author_sort |
Bigarani, Rocío |
| title |
Sex differences in active DNA demethylation machinery during the critical period of brain masculinization |
| title_short |
Sex differences in active DNA demethylation machinery during the critical period of brain masculinization |
| title_full |
Sex differences in active DNA demethylation machinery during the critical period of brain masculinization |
| title_fullStr |
Sex differences in active DNA demethylation machinery during the critical period of brain masculinization |
| title_full_unstemmed |
Sex differences in active DNA demethylation machinery during the critical period of brain masculinization |
| title_sort |
sex differences in active dna demethylation machinery during the critical period of brain masculinization |
| publishDate |
2022 |
| url |
http://hdl.handle.net/11086/549574 |
| work_keys_str_mv |
AT bigaranirocio sexdifferencesinactivednademethylationmachineryduringthecriticalperiodofbrainmasculinization AT villarrealmacarena sexdifferencesinactivednademethylationmachineryduringthecriticalperiodofbrainmasculinization AT cambiassomariajulia sexdifferencesinactivednademethylationmachineryduringthecriticalperiodofbrainmasculinization AT cisternascarladaniela sexdifferencesinactivednademethylationmachineryduringthecriticalperiodofbrainmasculinization |
| _version_ |
1809101988271489024 |
| spelling |
dig-unc-ar-11086-5495742024-07-26T18:22:20Z Sex differences in active DNA demethylation machinery during the critical period of brain masculinization Bigarani, Rocío Villarreal, Macarena Cambiasso, María Julia Cisternas, Carla Daniela DNA demethylation sexual differentiation epigenetics Fil: Bigarani, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Fil: Villarreal, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Fil: Cambiasso, María Julia. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Biología Celular B; Argentina. Fil: Cambiasso, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina Fil: Cisternas, Carla Daniela. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Biología Celular; Argentina. Fil: Cisternas, Carla Daniela. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Animal; Argentina. Fil: Cisternas, Carla Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. In mammals, perinatal peaks in gonadal testosterone organize a sex-typical neural circuitry during sensitive periods of development and a growing body of evidence suggest that epigenetic mechanisms are implicated. Some of the hormonal effects determine stable, sex-specific patterns of gene expression in neurons leading to the differentiation of neurochemical phenotypes relevant for the display of complex social behaviors in adulthood. We recently found that a neonatal inhibition of DNA methylation or demethylation reduces or eliminates sex differences in neurochemical phenotypes found in hypothalamic regions of the mouse brain. Here, we evaluated gene expression of TET 1-2-3, GAD45a-b and TDG (involved in the removal and replacement of 5-methylcytosine and 5-hydroxymethylcytosine) and the mRNA expression of the oxytocin receptor (OTR). mRNA expression was evaluated by qPCR in brain punches of prefrontal cortex (PFC) and preoptic area (POA) at postnatal day (P) 7 and P20. In PFC, we found sex differences (males > females) in TET3, TDG and Gad45b expression (p<0.05) and a trend for higher OTR-expression in males (p=0.06) at P7 suggesting higher DNA demethylation during the critical period of sexual differentiation. No sex differences were found at P20. Other brain regions and oxytocin expression are being evaluated. Overall, these results suggest that a sex-specific pattern of active DNA demethylation machinery could underline the organizational effects of hormones. https://san2022.saneurociencias.org.ar Fil: Bigarani, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Fil: Villarreal, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Fil: Cambiasso, María Julia. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Biología Celular B; Argentina. Fil: Cambiasso, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina Fil: Cisternas, Carla Daniela. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Biología Celular; Argentina. Fil: Cisternas, Carla Daniela. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Animal; Argentina. Fil: Cisternas, Carla Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina. Bioquímica y Biología Molecular (ídem 3.1.10) 2023-10-20T15:16:23Z 2023-10-20T15:16:23Z 2022 conferenceObject http://hdl.handle.net/11086/549574 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ Electrónico y/o Digital |