Connexin 43 induces odontoblast differentiation in rat incisors with dental fluorosis
Fil: Fontanetti, Pablo Alejandro. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Introducción a la Química y Física Biológicas A; Argentina.
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2016
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| Subjects: | fluorosis; connexin43; molecular mechanism, |
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fluorosis; connexin43; molecular mechanism fluorosis; connexin43; molecular mechanism Fontanetti, Pablo Alejandro Gallará, Raquel Vivian Interlandi, Victoria Ponce, Rubén Hugo Rigalli, Alfredo Centeno, Viviana Andrea Connexin 43 induces odontoblast differentiation in rat incisors with dental fluorosis |
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Fil: Fontanetti, Pablo Alejandro. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Introducción a la Química y Física Biológicas A; Argentina. |
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conferenceObject |
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fluorosis; connexin43; molecular mechanism |
| author |
Fontanetti, Pablo Alejandro Gallará, Raquel Vivian Interlandi, Victoria Ponce, Rubén Hugo Rigalli, Alfredo Centeno, Viviana Andrea |
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Fontanetti, Pablo Alejandro Gallará, Raquel Vivian Interlandi, Victoria Ponce, Rubén Hugo Rigalli, Alfredo Centeno, Viviana Andrea |
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Fontanetti, Pablo Alejandro |
| title |
Connexin 43 induces odontoblast differentiation in rat incisors with dental fluorosis |
| title_short |
Connexin 43 induces odontoblast differentiation in rat incisors with dental fluorosis |
| title_full |
Connexin 43 induces odontoblast differentiation in rat incisors with dental fluorosis |
| title_fullStr |
Connexin 43 induces odontoblast differentiation in rat incisors with dental fluorosis |
| title_full_unstemmed |
Connexin 43 induces odontoblast differentiation in rat incisors with dental fluorosis |
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connexin 43 induces odontoblast differentiation in rat incisors with dental fluorosis |
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2016 |
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http://hdl.handle.net/11086/547994 |
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AT fontanettipabloalejandro connexin43inducesodontoblastdifferentiationinratincisorswithdentalfluorosis AT gallararaquelvivian connexin43inducesodontoblastdifferentiationinratincisorswithdentalfluorosis AT interlandivictoria connexin43inducesodontoblastdifferentiationinratincisorswithdentalfluorosis AT poncerubenhugo connexin43inducesodontoblastdifferentiationinratincisorswithdentalfluorosis AT rigallialfredo connexin43inducesodontoblastdifferentiationinratincisorswithdentalfluorosis AT centenovivianaandrea connexin43inducesodontoblastdifferentiationinratincisorswithdentalfluorosis |
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1806029605183684608 |
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dig-unc-ar-11086-5479942024-07-02T18:28:14Z Connexin 43 induces odontoblast differentiation in rat incisors with dental fluorosis Fontanetti, Pablo Alejandro Gallará, Raquel Vivian Interlandi, Victoria Ponce, Rubén Hugo Rigalli, Alfredo Centeno, Viviana Andrea fluorosis; connexin43; molecular mechanism Fil: Fontanetti, Pablo Alejandro. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Introducción a la Química y Física Biológicas A; Argentina. Fil: Gallará, Raquel Vivian. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina. Fil: Interlandi, Victoria. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina. Fil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina. Fil: Rigalli, Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Fil: Centeno, Viviana Andrea. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina. Molecules of intercellular communication, such as connexin 43 (Cx43), are important during dentinogenesis since they control cellular growth and differentiation. Excessive ingestion of fluoride (F-) during the phase of dental mineralization generates alterations in the structure and function of cells from different dental tissues. In previous works, we demonstrated that chronic ingestion of F- through drinking water produced an increase in the activity of alkaline phosphatase and in the levels of ARNm of Cx43, osteocalcin and dentin sialophosphoprotein in pulp of rat´s incisor. OBJECTIVE: To study the effect of chronic exposure to F- on the protein expression of Cx43 and morphological and molecular indicators of odontoblast differentiation. METHODS: Male Wistar rats (n=6 per group) drank water during 8 weeks, with different concentrations of F- in the form of NaF: a) 0.3 mg/L (Control), b) 10 mg/L (Treated). Subsequently, all animals were euthanized; their mandibles were extracted for histological processing and the upper maxilla to obtain incisor´s pulp tissue. Protein expression of Cx43 was analyzed by immunohistochemistry. On digital microphotographs, histomorphometric parameters were analyzed: number of odontoblasts/µm2, ratio nucleus/cytoplasm, and predentin thickness. Total ARN was extracted from pulp tissue and gene expression of Hsp25, specific marker of odontoblast differentiation, was analyzed by RT-PCR. Data were analyzed by Student ?t? test. RESULTS: Immunoreactivity of Cx43 was evident in odontoblasts and increased by exposure to F- compared to controls. The F- dose used in the treated group did not modify the number of odontoblasts/ µm2, although it increased odontoblast differentiation, evidenced by the decrease in the ratio nucleus/cytoplasm of odontoblasts and expression of the differentiation marker Hsp25. CONCLUSION: Chronic exposure to high dose of F- could induce odontoblast differentiation mediated by Cx43, producing cells more capable to express genes and/or proteins essential for mineralization process.This work was funded by SECYT-UNC Y CICYT-UNLAR. https://iadr.abstractarchives.com/abstract/16iags-2503100/connexin-43-induces-odontoblast-differentiation-in-incisors-of-rats-with-dental-fluorosis Fil: Fontanetti, Pablo Alejandro. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Introducción a la Química y Física Biológicas A; Argentina. Fil: Gallará, Raquel Vivian. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina. Fil: Interlandi, Victoria. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina. Fil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina. Fil: Rigalli, Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina. Fil: Centeno, Viviana Andrea. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina. Otras Ciencias de la Salud 2023-07-03T12:52:53Z 2023-07-03T12:52:53Z 2016 conferenceObject http://hdl.handle.net/11086/547994 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ Impreso; Electrónico y/o Digital |