Evaluation of genotypic correlations of nitrogen-fixing bacteria in common beans (Phaseolus vulgaris L.).

The common bean (Phaseolus vulgaris L.) is a pulse and legume of significant agronomic importance because of its high nutritive values and ability to fix atmospheric nitrogen. It is grown across the world in America, Australia, Europe, the Middle East, Asia and Africa. In this research, a greenhouse experiment was conducted to evaluate the genotypic correlations of nitrogen-fixing bacteria (Rhizobium) in common beans. The red common bean Chirripo variety was used with seven treatments with five replicates of each: two with Bradyrhizobium japonicum, two with Rhizobium sp., one soil treatment, one substrate treatment receiving nitrogen and the control, which was only substrate. Six bacteria isolates were made and amplified by polymerase chain reaction (PCR). The 16S rRNA sequence were used to identify the isolated Rhizobia and assess their evolutionary relationship. The results obtained showed that the inoculum we had as Bradyrhizobium japonicum was actually Bradyrhizobium sp. It had excellent results, producing big and red nodules. Rhizobium sp. produced small nodules but effectively fixed nitrogen. In addition, the soils from the EARTH University Organic Farm produced nodules, though in smaller quantities. Thus, Bradyrhizobium sp. can be a successful alternative strain for common bean inoculation. The obtained phylogenetic results of the 16S rRNA show that the isolated Rhizobia were closely related to the genus Rhizobium, which is known for forming the symbiotic relationship with common beans: Rhizobium sp., Rhizobium tropici, and Bradyrhizobium sp. Common beans proved to be promiscuous (multi-host plant).

Bibliographic Details

Main Authors:	Shareka, Forget, Dilbato, Lidia
Other Authors:	Tamayo, Ana Cristina
Format:	info:eu-repo/semantics/bachelorThesis biblioteca
Language:	eng
Published:	Universidad EARTH 2019-12
Subjects:	PHASEOLUS VULGARIS, FRIJOL (PHASEOLUS), BACTERIAS FIJADORAS DE NITROGENO, BRADYRHIZOBIUM JAPONICUM, RHIZOBIUM, https://purl.org/pe-repo/ocde/ford#4.01.01,
Online Access:	https://repositorio.earth.ac.cr/handle/UEARTH/441
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