Study of the cross-talk between Fasciola hepatica juveniles and the intestinal epithelial cells of the host by transcriptomics in an in vitro model

Study of the cross-talk between Fasciola hepatica juveniles and the intestinal epithelial cells of the host by transcriptomics in an in vitro model

9 páginas, 5 figuras

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Bibliographic Details
Main Authors: Becerro Recio, David, Serrat, Judit, López-García, Marta, Torres Valle, María, Colina, Francisco, Fernández, Iván M, González Miguel, Javier, Siles Lucas, Mar
Other Authors: Ministerio de Ciencia, Innovación y Universidades (España)
Format: artículo biblioteca
Language:English
Published: Elsevier 2023
Subjects:Differential gene expression, Fasciola hepatica, Intestinal epithelium, Newly excysted juveniles, RNA-Seq, In vitro model,
Online Access:http://hdl.handle.net/10261/332411
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record_format koha
institution IRNASA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-irnasa-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IRNASA España
language English
topic Differential gene expression
Fasciola hepatica
Intestinal epithelium
Newly excysted juveniles
RNA-Seq
In vitro model
Differential gene expression
Fasciola hepatica
Intestinal epithelium
Newly excysted juveniles
RNA-Seq
In vitro model
spellingShingle Differential gene expression
Fasciola hepatica
Intestinal epithelium
Newly excysted juveniles
RNA-Seq
In vitro model
Differential gene expression
Fasciola hepatica
Intestinal epithelium
Newly excysted juveniles
RNA-Seq
In vitro model
Becerro Recio, David
Serrat, Judit
López-García, Marta
Torres Valle, María
Colina, Francisco
Fernández, Iván M
González Miguel, Javier
Siles Lucas, Mar
Study of the cross-talk between Fasciola hepatica juveniles and the intestinal epithelial cells of the host by transcriptomics in an in vitro model
description 9 páginas, 5 figuras
author2 Ministerio de Ciencia, Innovación y Universidades (España)
author_facet Ministerio de Ciencia, Innovación y Universidades (España)
Becerro Recio, David
Serrat, Judit
López-García, Marta
Torres Valle, María
Colina, Francisco
Fernández, Iván M
González Miguel, Javier
Siles Lucas, Mar
format artículo
topic_facet Differential gene expression
Fasciola hepatica
Intestinal epithelium
Newly excysted juveniles
RNA-Seq
In vitro model
author Becerro Recio, David
Serrat, Judit
López-García, Marta
Torres Valle, María
Colina, Francisco
Fernández, Iván M
González Miguel, Javier
Siles Lucas, Mar
author_sort Becerro Recio, David
title Study of the cross-talk between Fasciola hepatica juveniles and the intestinal epithelial cells of the host by transcriptomics in an in vitro model
title_short Study of the cross-talk between Fasciola hepatica juveniles and the intestinal epithelial cells of the host by transcriptomics in an in vitro model
title_full Study of the cross-talk between Fasciola hepatica juveniles and the intestinal epithelial cells of the host by transcriptomics in an in vitro model
title_fullStr Study of the cross-talk between Fasciola hepatica juveniles and the intestinal epithelial cells of the host by transcriptomics in an in vitro model
title_full_unstemmed Study of the cross-talk between Fasciola hepatica juveniles and the intestinal epithelial cells of the host by transcriptomics in an in vitro model
title_sort study of the cross-talk between fasciola hepatica juveniles and the intestinal epithelial cells of the host by transcriptomics in an in vitro model
publisher Elsevier
publishDate 2023
url http://hdl.handle.net/10261/332411
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spelling dig-irnasa-es-10261-3324112023-08-03T09:18:08Z Study of the cross-talk between Fasciola hepatica juveniles and the intestinal epithelial cells of the host by transcriptomics in an in vitro model Becerro Recio, David Serrat, Judit López-García, Marta Torres Valle, María Colina, Francisco Fernández, Iván M González Miguel, Javier Siles Lucas, Mar Ministerio de Ciencia, Innovación y Universidades (España) Junta de Castilla y León Becerro Recio, David [0000-0001-8876-2592] Serrat, Judit [0000-0002-1182-1088] Torres Valle, María [0000-0003-0503-2688] González Miguel, Javier [0000-0003-4279-4761] Siles Lucas, Mar [0000-0002-1257-2562] Differential gene expression Fasciola hepatica Intestinal epithelium Newly excysted juveniles RNA-Seq In vitro model 9 páginas, 5 figuras Fasciolosis is a globally widespread trematodiasis with a major economic and veterinary impact. Therefore, this disease is responsible for millions of dollars in losses to the livestock industry, and also constitutes an emerging human health problem in endemic areas. The ubiquitous nature of Fasciola hepatica, the main causative agent, is one of the key factors for the success of fasciolosis. Accordingly, this parasite is able to subsist in a wide variety of ecosystems and hosts, thanks to the development of a plethora of strategies for adaption and immune evasion. Fasciolosis comprises a growing concern due to its high prevalence rates, together with the emergence of strains of the parasite resistant to the treatment of choice (triclabendazole). These facts highlight the importance of developing novel control measures which allow for an effective protection against the disease before F. hepatica settles in a niche inaccessible to the immune system. However, knowledge about the initial phases of the infection, including the migration mechanisms of the parasite and the early innate host response, is still scarce. Recently, our group developed an in vitro host-parasite interaction model that allowed the early events to be unveiled after the first contact between the both actors. This occurs shortly upon ingestion of F. hepatica metacercariae and the emergence of the newly excysted juveniles (FhNEJ) in the host duodenum. Here, we present a transcriptomic analysis of such model using an approach based on RNA sequencing (RNA-Seq), which reveals changes in gene expression related to proteolysis and uptake of metabolites in FhNEJ. Additionally, contact with the parasite triggered changes in host intestinal cells related to pseudogenes expression and host defence mechanisms, including immune response, among others. In sum, these results provide a better understanding of the early stages of fasciolosis at molecular level, and a pool of targets that could be used in future therapeutic strategies against the disease. M.S.L. acknowledges the financial support of the Spanish Ministry of Science and Innovation (grant numbers AGL2015-67023-C2-2-R and PID2019-108782RB-C22) and the Junta de Castilla y Leon ´ “IRNASA/ CSIC Unit of Excellence” co-financed by the European Union (ERDF “Europe drives our growth”) (grant number CLU-2019-05). D.B.R. and J. S. acknowledge the support of the Junta de Castilla y León for their Predoctoral contracts. M.L.G. acknowledges the support of the Spanish Ministry of Science and Innovation for her FPU Predoctoral contract. J. G.M. is supported by the ‘Ramón y Cajal’ program of the Spanish Ministerio de Ciencia e Innovación (grant number RYC2020-030575-I). M.T. V. acknowledges the support of the European Commission NextGenerationEU Fund (grant number EU 2020/2094), through CSIC’s Global Health Platform (PTI Salud Global) Peer reviewed 2023-08-03T09:18:08Z 2023-08-03T09:18:08Z 2023 artículo Veterinary Parasitology 320: 109981(2023) 0304-4017 http://hdl.handle.net/10261/332411 10.1016/j.vetpar.2023.109981 37450963 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108782RB-C22/ES/FASCIOLA HEPATICA: SOLO UN ATAQUE COORDINADO EN LINEA PUEDE DERROTAR AL MASTER DE LA EVASION/ Veterinary parasitology Publisher's version http://dx.doi.org/10.1016/j.vetpar.2023.109981 Sí open Elsevier

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