Nitrate is a negative signal for fructan synthesis, and the fructosyltransferase-inducing trehalose inhibits nitrogen and carbon assimilation in excised barley leaves
• Fructan biosynthesis in barley (Hordeum vulgare) has been shown to be upregulated by sugar signalling and downregulated by nitrogen. The relationship between these two regulations is investigated. • Excised third-leaves of barley were fed nitrate or glutamine under two light intensities. Other leaf blades were supplied in the dark for 24 h with nitrate and trehalose in the presence of validamycin A, a trehalase inhibitor. • In the light, nitrate, but not glutamine, decreased fructan contents and sucrose:fructan 6-fructosyltransferase protein without affecting the levels of sucrose and other carbohydrates. In darkened leaves, trehalose increased and nitrate decreased the fructan contents and total sucrose:fructosyltransferase activity without altering the concentration of sucrose. The effect on fructan contents of trehalose disappeared, whereas that of nitrate remained in subsequent incubations in water under light. Trehalose decreased and nitrate increased the light- and CO2-saturated rate of photosynthesis without significantly affecting the initial Rubisco (ribulose-1,5-bisphosphate carboxylase oxygenase) activity. Trehalose feeding decreased the activation of nitrate reductase and amino acid levels, and blocked the positive effect of nitrate on the maximal activity of this enzyme. • The results indicate that nitrate, and not a downstream metabolite, is a negative signal for fructan synthesis, independent from the positive sugar signalling and overriding it. Trehalose signalling inhibits nitrogen and carbon assimilation, at the same time, inducing fructosyltransferase activity.
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Wiley-Blackwell
2004-03
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Subjects: | Barley (Hordeum vulgare), Fructan, Fructosyltransferase, Nitrate, Nitrogen, Regulation, Trehalose, |
Online Access: | http://hdl.handle.net/10261/271235 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100014180 |
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dig-irnasa-es-10261-2712352022-07-16T02:39:38Z Nitrate is a negative signal for fructan synthesis, and the fructosyltransferase-inducing trehalose inhibits nitrogen and carbon assimilation in excised barley leaves Morcuende, Rosa Kostadinova, Svetla Pérez Pérez, Pilar Martín del Molino, I. Martínez-Carrasco, Rafael European Commission Junta de Castilla y León Barley (Hordeum vulgare) Fructan Fructosyltransferase Nitrate Nitrogen Regulation Trehalose • Fructan biosynthesis in barley (Hordeum vulgare) has been shown to be upregulated by sugar signalling and downregulated by nitrogen. The relationship between these two regulations is investigated. • Excised third-leaves of barley were fed nitrate or glutamine under two light intensities. Other leaf blades were supplied in the dark for 24 h with nitrate and trehalose in the presence of validamycin A, a trehalase inhibitor. • In the light, nitrate, but not glutamine, decreased fructan contents and sucrose:fructan 6-fructosyltransferase protein without affecting the levels of sucrose and other carbohydrates. In darkened leaves, trehalose increased and nitrate decreased the fructan contents and total sucrose:fructosyltransferase activity without altering the concentration of sucrose. The effect on fructan contents of trehalose disappeared, whereas that of nitrate remained in subsequent incubations in water under light. Trehalose decreased and nitrate increased the light- and CO2-saturated rate of photosynthesis without significantly affecting the initial Rubisco (ribulose-1,5-bisphosphate carboxylase oxygenase) activity. Trehalose feeding decreased the activation of nitrate reductase and amino acid levels, and blocked the positive effect of nitrate on the maximal activity of this enzyme. • The results indicate that nitrate, and not a downstream metabolite, is a negative signal for fructan synthesis, independent from the positive sugar signalling and overriding it. Trehalose signalling inhibits nitrogen and carbon assimilation, at the same time, inducing fructosyltransferase activity. This work was funded by the Spanish National Research and Development Programme, FEDER (1FD97-0468 grant),and the Junta de Castilla y León (CSI5/00F grant). S. K. wasthe recipient of a fellowship from the Spanish Agency forInternational Cooperation 2022-06-01T15:00:14Z 2022-06-01T15:00:14Z 2004-03 2022-06-01T15:00:14Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1046/j.1469-8137.2004.00990.x issn: 0028-646X e-issn: 1469-8137 New Phytologist 161(3): 749-759 (2004) http://hdl.handle.net/10261/271235 10.1046/j.1469-8137.2004.00990.x http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100014180 http://dx.doi.org/10.1046/j.1469-8137.2004.00990.x Sí none Wiley-Blackwell |
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Barley (Hordeum vulgare) Fructan Fructosyltransferase Nitrate Nitrogen Regulation Trehalose Barley (Hordeum vulgare) Fructan Fructosyltransferase Nitrate Nitrogen Regulation Trehalose |
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Barley (Hordeum vulgare) Fructan Fructosyltransferase Nitrate Nitrogen Regulation Trehalose Barley (Hordeum vulgare) Fructan Fructosyltransferase Nitrate Nitrogen Regulation Trehalose Morcuende, Rosa Kostadinova, Svetla Pérez Pérez, Pilar Martín del Molino, I. Martínez-Carrasco, Rafael Nitrate is a negative signal for fructan synthesis, and the fructosyltransferase-inducing trehalose inhibits nitrogen and carbon assimilation in excised barley leaves |
description |
• Fructan biosynthesis in barley (Hordeum vulgare) has been shown to be upregulated by sugar signalling and downregulated by nitrogen. The relationship between these two regulations is investigated. • Excised third-leaves of barley were fed nitrate or glutamine under two light intensities. Other leaf blades were supplied in the dark for 24 h with nitrate and trehalose in the presence of validamycin A, a trehalase inhibitor. • In the light, nitrate, but not glutamine, decreased fructan contents and sucrose:fructan 6-fructosyltransferase protein without affecting the levels of sucrose and other carbohydrates. In darkened leaves, trehalose increased and nitrate decreased the fructan contents and total sucrose:fructosyltransferase activity without altering the concentration of sucrose. The effect on fructan contents of trehalose disappeared, whereas that of nitrate remained in subsequent incubations in water under light. Trehalose decreased and nitrate increased the light- and CO2-saturated rate of photosynthesis without significantly affecting the initial Rubisco (ribulose-1,5-bisphosphate carboxylase oxygenase) activity. Trehalose feeding decreased the activation of nitrate reductase and amino acid levels, and blocked the positive effect of nitrate on the maximal activity of this enzyme. • The results indicate that nitrate, and not a downstream metabolite, is a negative signal for fructan synthesis, independent from the positive sugar signalling and overriding it. Trehalose signalling inhibits nitrogen and carbon assimilation, at the same time, inducing fructosyltransferase activity. |
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European Commission |
author_facet |
European Commission Morcuende, Rosa Kostadinova, Svetla Pérez Pérez, Pilar Martín del Molino, I. Martínez-Carrasco, Rafael |
format |
artículo |
topic_facet |
Barley (Hordeum vulgare) Fructan Fructosyltransferase Nitrate Nitrogen Regulation Trehalose |
author |
Morcuende, Rosa Kostadinova, Svetla Pérez Pérez, Pilar Martín del Molino, I. Martínez-Carrasco, Rafael |
author_sort |
Morcuende, Rosa |
title |
Nitrate is a negative signal for fructan synthesis, and the fructosyltransferase-inducing trehalose inhibits nitrogen and carbon assimilation in excised barley leaves |
title_short |
Nitrate is a negative signal for fructan synthesis, and the fructosyltransferase-inducing trehalose inhibits nitrogen and carbon assimilation in excised barley leaves |
title_full |
Nitrate is a negative signal for fructan synthesis, and the fructosyltransferase-inducing trehalose inhibits nitrogen and carbon assimilation in excised barley leaves |
title_fullStr |
Nitrate is a negative signal for fructan synthesis, and the fructosyltransferase-inducing trehalose inhibits nitrogen and carbon assimilation in excised barley leaves |
title_full_unstemmed |
Nitrate is a negative signal for fructan synthesis, and the fructosyltransferase-inducing trehalose inhibits nitrogen and carbon assimilation in excised barley leaves |
title_sort |
nitrate is a negative signal for fructan synthesis, and the fructosyltransferase-inducing trehalose inhibits nitrogen and carbon assimilation in excised barley leaves |
publisher |
Wiley-Blackwell |
publishDate |
2004-03 |
url |
http://hdl.handle.net/10261/271235 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100014180 |
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