Potential of Near Infrared Spectroscopy for the classification of fungal endophytes of grasses

Potential of Near Infrared Spectroscopy for the classification of fungal endophytes of grasses

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Resumen del trabajo presentado en el 11th International Conference on Near Infrared Spectroscopy, celebrado en Córdoba (España), del 6 al 11 de abril de 2021

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Main Authors: García Ciudad, Antonia, Zabalgogeazcoa, Iñigo, Vázquez de Aldana, Beatriz R., Canedo, M. M., García Criado, Balbino
Format: póster de congreso biblioteca
Published: 2003-04-07
Subjects:Fungal endophytes, Epichloe, Grasses, NIRS, Classification,
Online Access:http://hdl.handle.net/10261/267227
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spelling dig-irnasa-es-10261-2672272022-04-21T01:53:38Z Potential of Near Infrared Spectroscopy for the classification of fungal endophytes of grasses García Ciudad, Antonia Zabalgogeazcoa, Iñigo Vázquez de Aldana, Beatriz R. Canedo, M. M. García Criado, Balbino Fungal endophytes Epichloe Grasses NIRS Classification Resumen del trabajo presentado en el 11th International Conference on Near Infrared Spectroscopy, celebrado en Córdoba (España), del 6 al 11 de abril de 2021 Several species of fungal endophytes infect a large number of grass species. Infected plants do not show any symptom, but contain toxíc alkaloids. Sínce some endophyte specíes are very similar morphologically, identification of these fungi often involve tedious laboratory work such as nucleotide sequencíng of variable genes, a process which involves multiple steps and is expensive. We have studied the potential of near infrared reflectance spectroscopy (NIRS) to classífy two species of the endophytic genus Epichloe. Epichloe festucae infects red fescue (Festuca rubra) and other fine fescues, whíle E. typhina infects several grass specíes. For this study we isolated E. festucae from plants of red fescue and E. typhína from orchardgrass (Dactylis glomerata). Pure cultures of E. fes tuca e and E. typhina are virtually identical, in terms of morphology and radial growth rates. Without information about the host precedence of the fungus, the best way of identifying them is by analysis of partial sequences of ribosomal RNA genes. For this work, the NIR spectra of 189 samples derived from 25 isolates of E. festucae and 17 ot E. typhina were registered. Two thirds of the isolates of each species were used to develop a calibration by means of principal component analysis. The remaining samples were used tor validation. With the calibration obtaíned, both endophyte specíes could be clearly separated, each specíes being in a different cluster. With the absorbance data, in 93% of the trials the test set was well classified. and the first component explained 98.5% of the variance. The results obtained suggest that NIRS may have applications in the identification of morphologically similar fungal species. 2022-04-20T07:02:34Z 2022-04-20T07:02:34Z 2003-04-07 2022-04-20T07:02:34Z póster de congreso http://purl.org/coar/resource_type/c_6670 11th International Conference on Near Infrared Spectroscopy (2003) http://hdl.handle.net/10261/267227 Sí open
institution IRNASA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-irnasa-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IRNASA España
topic Fungal endophytes
Epichloe
Grasses
NIRS
Classification
Fungal endophytes
Epichloe
Grasses
NIRS
Classification
spellingShingle Fungal endophytes
Epichloe
Grasses
NIRS
Classification
Fungal endophytes
Epichloe
Grasses
NIRS
Classification
García Ciudad, Antonia
Zabalgogeazcoa, Iñigo
Vázquez de Aldana, Beatriz R.
Canedo, M. M.
García Criado, Balbino
Potential of Near Infrared Spectroscopy for the classification of fungal endophytes of grasses
description Resumen del trabajo presentado en el 11th International Conference on Near Infrared Spectroscopy, celebrado en Córdoba (España), del 6 al 11 de abril de 2021
format póster de congreso
topic_facet Fungal endophytes
Epichloe
Grasses
NIRS
Classification
author García Ciudad, Antonia
Zabalgogeazcoa, Iñigo
Vázquez de Aldana, Beatriz R.
Canedo, M. M.
García Criado, Balbino
author_facet García Ciudad, Antonia
Zabalgogeazcoa, Iñigo
Vázquez de Aldana, Beatriz R.
Canedo, M. M.
García Criado, Balbino
author_sort García Ciudad, Antonia
title Potential of Near Infrared Spectroscopy for the classification of fungal endophytes of grasses
title_short Potential of Near Infrared Spectroscopy for the classification of fungal endophytes of grasses
title_full Potential of Near Infrared Spectroscopy for the classification of fungal endophytes of grasses
title_fullStr Potential of Near Infrared Spectroscopy for the classification of fungal endophytes of grasses
title_full_unstemmed Potential of Near Infrared Spectroscopy for the classification of fungal endophytes of grasses
title_sort potential of near infrared spectroscopy for the classification of fungal endophytes of grasses
publishDate 2003-04-07
url http://hdl.handle.net/10261/267227
work_keys_str_mv AT garciaciudadantonia potentialofnearinfraredspectroscopyfortheclassificationoffungalendophytesofgrasses
AT zabalgogeazcoainigo potentialofnearinfraredspectroscopyfortheclassificationoffungalendophytesofgrasses
AT vazquezdealdanabeatrizr potentialofnearinfraredspectroscopyfortheclassificationoffungalendophytesofgrasses
AT canedomm potentialofnearinfraredspectroscopyfortheclassificationoffungalendophytesofgrasses
AT garciacriadobalbino potentialofnearinfraredspectroscopyfortheclassificationoffungalendophytesofgrasses
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