Recognition pattern of the fasciola hepatica excretome/secretome during the course of an experimental infection in sheep by 2d immunoproteomics
11 páginas, 1 figura, 1 tabla
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Multidisciplinary Digital Publishing Institute
2021
|
| Subjects: | Fasciola hepatica, Sheep, Excretory/secretory antigens, Immunoproteomics, |
| Online Access: | http://hdl.handle.net/10261/244912 http://dx.doi.org/10.13039/501100000780 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-irnasa-es-10261-244912 |
|---|---|
| record_format |
koha |
| spelling |
dig-irnasa-es-10261-2449122021-12-27T16:28:39Z Recognition pattern of the fasciola hepatica excretome/secretome during the course of an experimental infection in sheep by 2d immunoproteomics Becerro Recio, David González Miguel, Javier Ucero, Alberto Sotillo, Javier Martínez Moreno, Álvaro Pérez Arévalo, José Cwiklinski, Krystyna Dalton, John P. Siles Lucas, Mar European Commission González Miguel, Javier [0000-0003-4279-4761] Siles Lucas, Mar [0000-0002-1257-2562] Fasciola hepatica Sheep Excretory/secretory antigens Immunoproteomics 11 páginas, 1 figura, 1 tabla Excretory/secretory products released by helminth parasites have been widely studied for their diagnostic utility, immunomodulatory properties, as well as for their use as vaccines. Due to their location at the host/parasite interface, the characterization of parasite secretions is important to unravel the molecular interactions governing the relationships between helminth parasites and their hosts. In this study, the excretory/secretory products from adult worms of the trematode Fasciola hepatica (FhES) were employed in a combination of two-dimensional electrophoresis, immunoblot and mass spectrometry, to analyze the immune response elicited in sheep during the course of an experimental infection. Ten different immunogenic proteins from FhES recognized by serum samples from infected sheep at 4, 8, and/or 12 weeks post-infection were identified. Among these, different isoforms of cathepsin L and B, peroxiredoxin, calmodulin, or glutathione S-transferase were recognized from the beginning to the end of the experimental infection, suggesting their potential role as immunomodulatory antigens. Furthermore, four FhES proteins (C2H2-type domaincontaining protein, ferritin, superoxide dismutase, and globin-3) were identified for the first time as non-immunogenic proteins. These results may help to further understand host/parasite relationships in fasciolosis, and to identify potential diagnostic molecules and drug target candidates of F. hepatica. This research was funded by European Union’s Horizon 2020 research and innovation program under grant agreement No 635408 (PARAGONE) Peer reviewed 2021-06-29T18:47:21Z 2021-06-29T18:47:21Z 2021 artículo http://purl.org/coar/resource_type/c_6501 Pathogens 10(6): 725 (2021) http://hdl.handle.net/10261/244912 10.3390/pathogens10060725 2076-0817 http://dx.doi.org/10.13039/501100000780 34207550 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/635408 Publisher's version http://dx.doi.org/10.3390/pathogens10060725 Sí open Multidisciplinary Digital Publishing Institute |
| institution |
IRNASA ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-irnasa-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del IRNASA España |
| language |
English |
| topic |
Fasciola hepatica Sheep Excretory/secretory antigens Immunoproteomics Fasciola hepatica Sheep Excretory/secretory antigens Immunoproteomics |
| spellingShingle |
Fasciola hepatica Sheep Excretory/secretory antigens Immunoproteomics Fasciola hepatica Sheep Excretory/secretory antigens Immunoproteomics Becerro Recio, David González Miguel, Javier Ucero, Alberto Sotillo, Javier Martínez Moreno, Álvaro Pérez Arévalo, José Cwiklinski, Krystyna Dalton, John P. Siles Lucas, Mar Recognition pattern of the fasciola hepatica excretome/secretome during the course of an experimental infection in sheep by 2d immunoproteomics |
| description |
11 páginas, 1 figura, 1 tabla |
| author2 |
European Commission |
| author_facet |
European Commission Becerro Recio, David González Miguel, Javier Ucero, Alberto Sotillo, Javier Martínez Moreno, Álvaro Pérez Arévalo, José Cwiklinski, Krystyna Dalton, John P. Siles Lucas, Mar |
| format |
artículo |
| topic_facet |
Fasciola hepatica Sheep Excretory/secretory antigens Immunoproteomics |
| author |
Becerro Recio, David González Miguel, Javier Ucero, Alberto Sotillo, Javier Martínez Moreno, Álvaro Pérez Arévalo, José Cwiklinski, Krystyna Dalton, John P. Siles Lucas, Mar |
| author_sort |
Becerro Recio, David |
| title |
Recognition pattern of the fasciola hepatica excretome/secretome during the course of an experimental infection in sheep by 2d immunoproteomics |
| title_short |
Recognition pattern of the fasciola hepatica excretome/secretome during the course of an experimental infection in sheep by 2d immunoproteomics |
| title_full |
Recognition pattern of the fasciola hepatica excretome/secretome during the course of an experimental infection in sheep by 2d immunoproteomics |
| title_fullStr |
Recognition pattern of the fasciola hepatica excretome/secretome during the course of an experimental infection in sheep by 2d immunoproteomics |
| title_full_unstemmed |
Recognition pattern of the fasciola hepatica excretome/secretome during the course of an experimental infection in sheep by 2d immunoproteomics |
| title_sort |
recognition pattern of the fasciola hepatica excretome/secretome during the course of an experimental infection in sheep by 2d immunoproteomics |
| publisher |
Multidisciplinary Digital Publishing Institute |
| publishDate |
2021 |
| url |
http://hdl.handle.net/10261/244912 http://dx.doi.org/10.13039/501100000780 |
| work_keys_str_mv |
AT becerroreciodavid recognitionpatternofthefasciolahepaticaexcretomesecretomeduringthecourseofanexperimentalinfectioninsheepby2dimmunoproteomics AT gonzalezmigueljavier recognitionpatternofthefasciolahepaticaexcretomesecretomeduringthecourseofanexperimentalinfectioninsheepby2dimmunoproteomics AT uceroalberto recognitionpatternofthefasciolahepaticaexcretomesecretomeduringthecourseofanexperimentalinfectioninsheepby2dimmunoproteomics AT sotillojavier recognitionpatternofthefasciolahepaticaexcretomesecretomeduringthecourseofanexperimentalinfectioninsheepby2dimmunoproteomics AT martinezmorenoalvaro recognitionpatternofthefasciolahepaticaexcretomesecretomeduringthecourseofanexperimentalinfectioninsheepby2dimmunoproteomics AT perezarevalojose recognitionpatternofthefasciolahepaticaexcretomesecretomeduringthecourseofanexperimentalinfectioninsheepby2dimmunoproteomics AT cwiklinskikrystyna recognitionpatternofthefasciolahepaticaexcretomesecretomeduringthecourseofanexperimentalinfectioninsheepby2dimmunoproteomics AT daltonjohnp recognitionpatternofthefasciolahepaticaexcretomesecretomeduringthecourseofanexperimentalinfectioninsheepby2dimmunoproteomics AT sileslucasmar recognitionpatternofthefasciolahepaticaexcretomesecretomeduringthecourseofanexperimentalinfectioninsheepby2dimmunoproteomics |
| _version_ |
1777665405202989056 |