Strains nodulating Lupinus albus on different continents belong to several new chromosomal and symbiotic lineages within Bradyrhizobium
In this work we analysed different chromosomal and symbiotic markers in rhizobial strains nodulating Lupinus albus (white lupin) in several continents. Collectively the analysis of their rrs and atpD genes, and 16S-23S intergenic spacers (ITS), showed that they belong to at least four chromosomal lineages within the genus Bradyrhizobium. Most isolates from the Canary Islands (near to the African continent) grouped with some strains isolated on mainland Spain and were identified as Bradyrhizobium canariense. These strains are divided into two ITS subgroups coincident with those previously described from isolates nodulating Ornithopus. The remaining strains isolated on mainland Spain grouped with most isolates from Chile (American continent) forming a new lineage related to Bradyrhizobium japonicum. The strains BLUT2 and ISLU207 isolated from the Canary Islands and Chile, respectively, formed two new lineages phylogenetically close to different species of Bradyrhizobium depending on the marker analyzed. The analysis of the nodC gene showed that all strains nodulating L. albus belong to the biovar genistearum; nevertheless they form four different nodC lineages of which lineage C is at present exclusively formed by L. albus endosymbionts isolated from different continents.
| Main Authors: | , , , , , , , , , , |
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| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Springer
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| Subjects: | Bradyrhizobium, Lupinus, Phylogeny, |
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| Summary: | In this work we analysed different chromosomal
and symbiotic markers in rhizobial strains
nodulating Lupinus albus (white lupin) in several
continents. Collectively the analysis of their rrs and
atpD genes, and 16S-23S intergenic spacers (ITS),
showed that they belong to at least four chromosomal
lineages within the genus Bradyrhizobium. Most
isolates from the Canary Islands (near to the African
continent) grouped with some strains isolated on
mainland Spain and were identified as Bradyrhizobium
canariense. These strains are divided into two ITS
subgroups coincident with those previously described
from isolates nodulating Ornithopus. The remaining
strains isolated on mainland Spain grouped with most
isolates from Chile (American continent) forming a
new lineage related to Bradyrhizobium japonicum.
The strains BLUT2 and ISLU207 isolated from the
Canary Islands and Chile, respectively, formed two
new lineages phylogenetically close to different
species of Bradyrhizobium depending on the marker
analyzed. The analysis of the nodC gene showed that
all strains nodulating L. albus belong to the biovar
genistearum; nevertheless they form four different
nodC lineages of which lineage C is at present
exclusively formed by L. albus endosymbionts isolated
from different continents. |
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