Natural diversity of nodular microsymbionts of Alnus glutinosa in the Tormes River basin.
The genetic diversity of Frankia strains nodulating Alnus glutinosa along the basin of the Tormes River was studied on DNA extracted directly from nodules. Frankia strains inhabiting root nodules at 12 different locations, ranging in altitude from 409 to 1181 m, were characterized. For that, we amplified the whole IGS region between 16S–23S rDNA and performed a restriction fragment length polymorphism (RFLP) analysis with four restriction enzymes. Two different RFLP patterns (termed A and B) were obtained with HaeIII, indicating the existence of two different groups of Frankia strains. Three different nodule extracts from each of the two RFLP groups were selected for further analyses. Sequencing of the 16S–23S rDNA IGS showed a 100% of intragroup homology and also confirmed the difference (98.4% level of similarity) between the Frankia strains in the two nodule extract groups. The phylogenetic analyses based on the two 16S–23S rDNA IGS sequences obtained in this study and other previously published sequences indicated that Frankia strains TFAg5 and TFAg23 (chosen as representative of HaeIII–RFLP group A and B, respectively) are quite similar to other strains nodulating plants of A. rhombifolia and A. viridis in California (pairwise levels of similarity including gaps ranged from 97.8% to 98.6%), together with which they form a single group. To put the Frankia strains representative of each HaeIII–RFLP group in the context of overall Frankia diversity we amplified and sequenced the 16S rDNA and glnII gene from nodular DNA. An also remarkable fact found in this study was that Frankia strains belonging to the HaeIII–RFLP group A were distributed all along the river course, from the lowest site sampled to the highest, while Frankia strains placed into RFLP group B were restricted to the upper Tormes River, being exclusively found at altitudes of 946 m or higher.
Main Authors: | , , , , |
---|---|
Format: | artículo biblioteca |
Language: | English |
Published: |
Springer
|
Subjects: | Alnus glutinosa, Altitude, Frankia, Genetic diversity, Glutamine synthetase gene, 16S rRNA, 23S rRNA, |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-irnasa-es-10261-23834 |
---|---|
record_format |
koha |
spelling |
dig-irnasa-es-10261-238342020-06-01T08:56:28Z Natural diversity of nodular microsymbionts of Alnus glutinosa in the Tormes River basin. Igual, José Mariano Valverde Portal, Ángel Velázquez, Encarna Santa Regina, Ignacio Rodríguez Barrueco, Claudino Alnus glutinosa Altitude Frankia Genetic diversity Glutamine synthetase gene 16S rRNA 23S rRNA The genetic diversity of Frankia strains nodulating Alnus glutinosa along the basin of the Tormes River was studied on DNA extracted directly from nodules. Frankia strains inhabiting root nodules at 12 different locations, ranging in altitude from 409 to 1181 m, were characterized. For that, we amplified the whole IGS region between 16S–23S rDNA and performed a restriction fragment length polymorphism (RFLP) analysis with four restriction enzymes. Two different RFLP patterns (termed A and B) were obtained with HaeIII, indicating the existence of two different groups of Frankia strains. Three different nodule extracts from each of the two RFLP groups were selected for further analyses. Sequencing of the 16S–23S rDNA IGS showed a 100% of intragroup homology and also confirmed the difference (98.4% level of similarity) between the Frankia strains in the two nodule extract groups. The phylogenetic analyses based on the two 16S–23S rDNA IGS sequences obtained in this study and other previously published sequences indicated that Frankia strains TFAg5 and TFAg23 (chosen as representative of HaeIII–RFLP group A and B, respectively) are quite similar to other strains nodulating plants of A. rhombifolia and A. viridis in California (pairwise levels of similarity including gaps ranged from 97.8% to 98.6%), together with which they form a single group. To put the Frankia strains representative of each HaeIII–RFLP group in the context of overall Frankia diversity we amplified and sequenced the 16S rDNA and glnII gene from nodular DNA. An also remarkable fact found in this study was that Frankia strains belonging to the HaeIII–RFLP group A were distributed all along the river course, from the lowest site sampled to the highest, while Frankia strains placed into RFLP group B were restricted to the upper Tormes River, being exclusively found at altitudes of 946 m or higher. Peer reviewed 2006 artículo http://purl.org/coar/resource_type/c_6501 Plant and Soil (2006) 280 (1-2):373–383 0032-079X 10261/23834 10.1007/s11104-005-3700-7 en http://www.springerlink.com/content/412477135774w528/ open Springer |
institution |
IRNASA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-irnasa-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del IRNASA España |
language |
English |
topic |
Alnus glutinosa Altitude Frankia Genetic diversity Glutamine synthetase gene 16S rRNA 23S rRNA Alnus glutinosa Altitude Frankia Genetic diversity Glutamine synthetase gene 16S rRNA 23S rRNA |
spellingShingle |
Alnus glutinosa Altitude Frankia Genetic diversity Glutamine synthetase gene 16S rRNA 23S rRNA Alnus glutinosa Altitude Frankia Genetic diversity Glutamine synthetase gene 16S rRNA 23S rRNA Igual, José Mariano Valverde Portal, Ángel Velázquez, Encarna Santa Regina, Ignacio Rodríguez Barrueco, Claudino Natural diversity of nodular microsymbionts of Alnus glutinosa in the Tormes River basin. |
description |
The genetic diversity of Frankia strains nodulating Alnus glutinosa along the basin of the Tormes River was
studied on DNA extracted directly from nodules. Frankia strains inhabiting root nodules at 12 different
locations, ranging in altitude from 409 to 1181 m, were characterized. For that, we amplified the whole IGS
region between 16S–23S rDNA and performed a restriction fragment length polymorphism (RFLP)
analysis with four restriction enzymes. Two different RFLP patterns (termed A and B) were obtained with
HaeIII, indicating the existence of two different groups of Frankia strains. Three different nodule extracts
from each of the two RFLP groups were selected for further analyses. Sequencing of the 16S–23S rDNA
IGS showed a 100% of intragroup homology and also confirmed the difference (98.4% level of similarity)
between the Frankia strains in the two nodule extract groups. The phylogenetic analyses based on the two
16S–23S rDNA IGS sequences obtained in this study and other previously published sequences indicated
that Frankia strains TFAg5 and TFAg23 (chosen as representative of HaeIII–RFLP group A and B,
respectively) are quite similar to other strains nodulating plants of A. rhombifolia and A. viridis in California
(pairwise levels of similarity including gaps ranged from 97.8% to 98.6%), together with which they
form a single group. To put the Frankia strains representative of each HaeIII–RFLP group in the context of
overall Frankia diversity we amplified and sequenced the 16S rDNA and glnII gene from nodular DNA. An
also remarkable fact found in this study was that Frankia strains belonging to the HaeIII–RFLP group A
were distributed all along the river course, from the lowest site sampled to the highest, while Frankia strains
placed into RFLP group B were restricted to the upper Tormes River, being exclusively found at altitudes
of 946 m or higher. |
format |
artículo |
topic_facet |
Alnus glutinosa Altitude Frankia Genetic diversity Glutamine synthetase gene 16S rRNA 23S rRNA |
author |
Igual, José Mariano Valverde Portal, Ángel Velázquez, Encarna Santa Regina, Ignacio Rodríguez Barrueco, Claudino |
author_facet |
Igual, José Mariano Valverde Portal, Ángel Velázquez, Encarna Santa Regina, Ignacio Rodríguez Barrueco, Claudino |
author_sort |
Igual, José Mariano |
title |
Natural diversity of nodular microsymbionts of Alnus glutinosa in the Tormes River basin. |
title_short |
Natural diversity of nodular microsymbionts of Alnus glutinosa in the Tormes River basin. |
title_full |
Natural diversity of nodular microsymbionts of Alnus glutinosa in the Tormes River basin. |
title_fullStr |
Natural diversity of nodular microsymbionts of Alnus glutinosa in the Tormes River basin. |
title_full_unstemmed |
Natural diversity of nodular microsymbionts of Alnus glutinosa in the Tormes River basin. |
title_sort |
natural diversity of nodular microsymbionts of alnus glutinosa in the tormes river basin. |
publisher |
Springer |
work_keys_str_mv |
AT igualjosemariano naturaldiversityofnodularmicrosymbiontsofalnusglutinosainthetormesriverbasin AT valverdeportalangel naturaldiversityofnodularmicrosymbiontsofalnusglutinosainthetormesriverbasin AT velazquezencarna naturaldiversityofnodularmicrosymbiontsofalnusglutinosainthetormesriverbasin AT santareginaignacio naturaldiversityofnodularmicrosymbiontsofalnusglutinosainthetormesriverbasin AT rodriguezbarruecoclaudino naturaldiversityofnodularmicrosymbiontsofalnusglutinosainthetormesriverbasin |
_version_ |
1777665226698653696 |