Proteomic analysis of plasma exosomes from Cystic Echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers

Proteomic analysis of plasma exosomes from Cystic Echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers

31 páginas, 3 tablas, 10 figuras

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Main Authors: Fratini, Federica, Tamarozzi, Francesca, Macchia, G., Bertuccini, L., Mariconti, M., Birago, C., Iriarte, A., Brunetti, Enrico, Cretu, Carmen Michaela, Akhan, Okan, Siles Lucas, Mar, Diaz, A., Casulli, Adriano
Other Authors: European Commission
Format: artículo biblioteca
Language:English
Published: Public Library of Science 2020
Subjects:Cystic echinococcosis, Circulating biomarkers, Exosomes, Diagnosis,
Online Access:http://hdl.handle.net/10261/221974
http://dx.doi.org/10.13039/501100000780
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spelling dig-irnasa-es-10261-2219742023-02-07T13:44:26Z Proteomic analysis of plasma exosomes from Cystic Echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers Fratini, Federica Tamarozzi, Francesca Macchia, G. Bertuccini, L. Mariconti, M. Birago, C. Iriarte, A. Brunetti, Enrico Cretu, Carmen Michaela Akhan, Okan Siles Lucas, Mar Diaz, A. Casulli, Adriano European Commission Siles Lucas, Mar [0000-0002-1257-2562] Cystic echinococcosis Circulating biomarkers Exosomes Diagnosis 31 páginas, 3 tablas, 10 figuras The reference diagnostic method of human abdominal Cystic Echinococcosis (CE) is imaging, particularly ultrasound, supported by serology when imaging is inconclusive. However, current diagnostic tools are neither optimal nor widely available. The availability of a test detecting circulating biomarkers would considerably improve CE diagnosis and cyst staging (active vs inactive), as well as treatments and follow-up of patients. Exosomes are extracellular vesicles involved in intercellular communication, including immune system responses, and are a recognized source of biomarkers. With the aim of identifying potential biomarkers, plasma pools from patients infected by active or inactive CE, as well as from control subjects, were processed to isolate exosomes for proteomic label-free quantitative analysis. Results were statistically processed and subjected to bioinformatics analysis to define distinct features associated with parasite viability. First, a few parasite proteins were identified that were specifically associated with either active or inactive CE, which represent potential biomarkers to be validated in further studies. Second, numerous identified proteins of human origin were common to active and inactive CE, confirming an overlap of several immune response pathways. However, a subset of human proteins specific to either active or inactive CE, and central in the respective protein-protein interaction networks, were identified. These include the Src family kinases Src and Lyn, and the immune-suppressive cytokine TGF-β in active CE, and Cdc42 in inactive CE. The Src and Lyn Kinases were confirmed as potential markers of active CE in totally independent plasma pools. In addition, insights were obtained on immune response profiles: largely consistent with previous evidence, our observations hint to a Th1/Th2/regulatory immune environment in patients with active CE and a Th1/inflammatory environment with a component of the wound healing response in the presence of inactive CE. Of note, our results were obtained for the first time from the analysis of samples obtained in vivo from a well-characterized, large cohort of human subjects. This work was funded by the European Commission Seventh Framework Programme (FP7) under the HERACLES project (grant agreement n 602051) Peer reviewed 2020-10-29T08:42:23Z 2020-10-29T08:42:23Z 2020 artículo http://purl.org/coar/resource_type/c_6501 PLoS Neglected Tropical Diseases, 14: e0008586 (2020) 1935-2727 http://hdl.handle.net/10261/221974 10.1371/journal.pntd.0008586 1935-2735 http://dx.doi.org/10.13039/501100000780 33017416 en #PLACEHOLDER_PARENT_METADATA_VALUE# eu-repo/grantAgreement/EC/FP7/602051 Publisher's version https://doi.org/10.1371/journal.pntd.0008586 Sí open Public Library of Science
institution IRNASA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-irnasa-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IRNASA España
language English
topic Cystic echinococcosis
Circulating biomarkers
Exosomes
Diagnosis
Cystic echinococcosis
Circulating biomarkers
Exosomes
Diagnosis
spellingShingle Cystic echinococcosis
Circulating biomarkers
Exosomes
Diagnosis
Cystic echinococcosis
Circulating biomarkers
Exosomes
Diagnosis
Fratini, Federica
Tamarozzi, Francesca
Macchia, G.
Bertuccini, L.
Mariconti, M.
Birago, C.
Iriarte, A.
Brunetti, Enrico
Cretu, Carmen Michaela
Akhan, Okan
Siles Lucas, Mar
Diaz, A.
Casulli, Adriano
Proteomic analysis of plasma exosomes from Cystic Echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers
description 31 páginas, 3 tablas, 10 figuras
author2 European Commission
author_facet European Commission
Fratini, Federica
Tamarozzi, Francesca
Macchia, G.
Bertuccini, L.
Mariconti, M.
Birago, C.
Iriarte, A.
Brunetti, Enrico
Cretu, Carmen Michaela
Akhan, Okan
Siles Lucas, Mar
Diaz, A.
Casulli, Adriano
format artículo
topic_facet Cystic echinococcosis
Circulating biomarkers
Exosomes
Diagnosis
author Fratini, Federica
Tamarozzi, Francesca
Macchia, G.
Bertuccini, L.
Mariconti, M.
Birago, C.
Iriarte, A.
Brunetti, Enrico
Cretu, Carmen Michaela
Akhan, Okan
Siles Lucas, Mar
Diaz, A.
Casulli, Adriano
author_sort Fratini, Federica
title Proteomic analysis of plasma exosomes from Cystic Echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers
title_short Proteomic analysis of plasma exosomes from Cystic Echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers
title_full Proteomic analysis of plasma exosomes from Cystic Echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers
title_fullStr Proteomic analysis of plasma exosomes from Cystic Echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers
title_full_unstemmed Proteomic analysis of plasma exosomes from Cystic Echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers
title_sort proteomic analysis of plasma exosomes from cystic echinococcosis patients provides in vivo support for distinct immune response profiles in active vs inactive infection and suggests potential biomarkers
publisher Public Library of Science
publishDate 2020
url http://hdl.handle.net/10261/221974
http://dx.doi.org/10.13039/501100000780
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