Discovery of phloeophagus beetles as a source of pseudomonas strains that produce potentially new bioactive substances and description of pseudomonas bohemica sp. nov.

Saati-Santamaría, Zaki; López-Mondéjar, Rubén; Jiménez-Gómez, Alejandro; Díez-Méndez, Alexandra; Vetrovský, Tomáš; Igual, José Mariano; Velázquez, Encarna; Kolařik, Miroslav; Rivas, Raúl; García-Fraile, Paula; Czech Science Foundation

Discovery of phloeophagus beetles as a source of pseudomonas strains that produce potentially new bioactive substances and description of pseudomonas bohemica sp. nov.

15 páginas, 6 tablas, 2 figuras. -- First publication is due to Frontiers Media

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Bibliographic Details
Main Authors:	Saati-Santamaría, Zaki, López-Mondéjar, Rubén, Jiménez-Gómez, Alejandro, Díez-Méndez, Alexandra, Vetrovský, Tomáš, Igual, José Mariano, Velázquez, Encarna, Kolařik, Miroslav, Rivas, Raúl, García-Fraile, Paula
Other Authors:	Czech Science Foundation
Format:	artículo biblioteca
Language:	English
Published:	Frontiers Media 2018
Subjects:	Antimicrobials, Anticarcinogenic, Antiviral, Genome mining, Bark beetles, Antibiotic resistance, NRPS-PKS, Secondary metabolites,
Online Access:	http://hdl.handle.net/10261/164965
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spelling	dig-irnasa-es-10261-1649652022-10-06T06:28:56Z Discovery of phloeophagus beetles as a source of pseudomonas strains that produce potentially new bioactive substances and description of pseudomonas bohemica sp. nov. Saati-Santamaría, Zaki López-Mondéjar, Rubén Jiménez-Gómez, Alejandro Díez-Méndez, Alexandra Vetrovský, Tomáš Igual, José Mariano Velázquez, Encarna Kolařik, Miroslav Rivas, Raúl García-Fraile, Paula Czech Science Foundation Igual, José Mariano [0000-0002-5080-0378] Antimicrobials Anticarcinogenic Antiviral Genome mining Bark beetles Antibiotic resistance NRPS-PKS Secondary metabolites 15 páginas, 6 tablas, 2 figuras. -- First publication is due to Frontiers Media Antimicrobial resistance is a worldwide problem that threatens the effectiveness of treatments for microbial infection. Consequently, it is essential to study unexplored niches that can serve for the isolation of new microbial strains able to produce antimicrobial compounds to develop new drugs. Bark beetles live in phloem of host trees and establish symbioses with microorganisms that provide them with nutrients. In addition, some of their associated bacteria play a role in the beetle protection by producing substances that inhibit antagonists. In this study the capacity of several bacterial strains, isolated from the bark beetles Ips acuminatus, Pityophthorus pityographus Cryphalus piceae, and Pityogenes bidentatus, to produce antimicrobial compounds was analyzed. Several isolates exhibited the capacity to inhibit Gram-positive and Gram-negative bacteria, as well as fungi. The genome sequence analysis of three Pseudomonas isolates predicted the presence of several gene clusters implicated in the production of already described antimicrobials and moreover, the low similarity of some of these clusters with those previously described, suggests that they encode new undescribed substances, which may be useful for developing new antimicrobial agents. Moreover, these bacteria appear to have genetic machinery for producing antitumoral and antiviral substances. Finally, the strain IA19T showed to represent a new species of the genus Pseudomonas. The 16S rRNA gene sequence analysis showed that its most closely related species include Pseudomonas lutea, Pseudomonas graminis, Pseudomonas abietaniphila and Pseudomonas alkylphenolica, with 98.6, 98.5 98.4, and 98.4% identity, respectively. MLSA of the housekeeping genes gyrB, rpoB, and rpoD confirmed that strain IA19T clearly separates from its closest related species. Average nucleotide identity between strains IA19T and P. abietaniphila ATCC 700689T, P. graminis DSM 11363T, P. alkylphenolica KL28T and P. lutea DSM 17257T were 85.3, 80.2, 79.0, and 72.1%, respectively. Growth occurs at 4-37°C and pH 6.5-8. Optimal growth occurs at 28°C, pH 7–8 and up to 2.5% NaCl. Respiratory ubiquinones are Q9 (97%) and Q8 (3%). C16:0 and in summed feature 3 are the main fatty acids. Based on genotypic, phenotypic and chemotaxonomic characteristics, the description of Pseudomonas bohemica sp. nov. has been proposed. The type strain is IA19T (=CECT 9403T = LMG 30182T). This work was supported by the Czech Science Foundation (GACR) under the project number 16-15293Y. Peer reviewed 2018-05-22T10:14:43Z 2018-05-22T10:14:43Z 2018 artículo http://purl.org/coar/resource_type/c_6501 Frontiers in Microbiology, 9: 913 1664-302X http://hdl.handle.net/10261/164965 10.3389/fmicb.2018.00913 29867824 en Publisher's version http://dx.doi.org/10.3389/fmicb.2018.00913 Sí open Frontiers Media
institution	IRNASA ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-irnasa-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del IRNASA España
language	English
topic	Antimicrobials Anticarcinogenic Antiviral Genome mining Bark beetles Antibiotic resistance NRPS-PKS Secondary metabolites Antimicrobials Anticarcinogenic Antiviral Genome mining Bark beetles Antibiotic resistance NRPS-PKS Secondary metabolites
spellingShingle	Antimicrobials Anticarcinogenic Antiviral Genome mining Bark beetles Antibiotic resistance NRPS-PKS Secondary metabolites Antimicrobials Anticarcinogenic Antiviral Genome mining Bark beetles Antibiotic resistance NRPS-PKS Secondary metabolites Saati-Santamaría, Zaki López-Mondéjar, Rubén Jiménez-Gómez, Alejandro Díez-Méndez, Alexandra Vetrovský, Tomáš Igual, José Mariano Velázquez, Encarna Kolařik, Miroslav Rivas, Raúl García-Fraile, Paula Discovery of phloeophagus beetles as a source of pseudomonas strains that produce potentially new bioactive substances and description of pseudomonas bohemica sp. nov.
description	15 páginas, 6 tablas, 2 figuras. -- First publication is due to Frontiers Media
author2	Czech Science Foundation
author_facet	Czech Science Foundation Saati-Santamaría, Zaki López-Mondéjar, Rubén Jiménez-Gómez, Alejandro Díez-Méndez, Alexandra Vetrovský, Tomáš Igual, José Mariano Velázquez, Encarna Kolařik, Miroslav Rivas, Raúl García-Fraile, Paula
format	artículo
topic_facet	Antimicrobials Anticarcinogenic Antiviral Genome mining Bark beetles Antibiotic resistance NRPS-PKS Secondary metabolites
author	Saati-Santamaría, Zaki López-Mondéjar, Rubén Jiménez-Gómez, Alejandro Díez-Méndez, Alexandra Vetrovský, Tomáš Igual, José Mariano Velázquez, Encarna Kolařik, Miroslav Rivas, Raúl García-Fraile, Paula
author_sort	Saati-Santamaría, Zaki
title	Discovery of phloeophagus beetles as a source of pseudomonas strains that produce potentially new bioactive substances and description of pseudomonas bohemica sp. nov.
title_short	Discovery of phloeophagus beetles as a source of pseudomonas strains that produce potentially new bioactive substances and description of pseudomonas bohemica sp. nov.
title_full	Discovery of phloeophagus beetles as a source of pseudomonas strains that produce potentially new bioactive substances and description of pseudomonas bohemica sp. nov.
title_fullStr	Discovery of phloeophagus beetles as a source of pseudomonas strains that produce potentially new bioactive substances and description of pseudomonas bohemica sp. nov.
title_full_unstemmed	Discovery of phloeophagus beetles as a source of pseudomonas strains that produce potentially new bioactive substances and description of pseudomonas bohemica sp. nov.
title_sort	discovery of phloeophagus beetles as a source of pseudomonas strains that produce potentially new bioactive substances and description of pseudomonas bohemica sp. nov.
publisher	Frontiers Media
publishDate	2018
url	http://hdl.handle.net/10261/164965
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Discovery of phloeophagus beetles as a source of pseudomonas strains that produce potentially new bioactive substances and description of pseudomonas bohemica sp. nov.

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