Transcriptome and metabolome analysis of plant sulfate starvation and resupply provides novel information on transcriptional regulation of metabolism associated with sulfur, nitrogen and phosphorus nutritional responses in Arabidopsis
18 páginas, 7 figuras, 1 tabla
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| Format: | artículo biblioteca |
| Language: | English |
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Frontiers Media
2015
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| Subjects: | Sulfur, Sulfate starvation, Nitrate, Phosphate, Transcription factor, Microarray, Metabolomics, Transcriptomics, |
| Online Access: | http://hdl.handle.net/10261/132042 http://dx.doi.org/10.13039/501100000268 http://dx.doi.org/10.13039/501100004189 |
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dig-irnasa-es-10261-1320422021-12-28T16:31:39Z Transcriptome and metabolome analysis of plant sulfate starvation and resupply provides novel information on transcriptional regulation of metabolism associated with sulfur, nitrogen and phosphorus nutritional responses in Arabidopsis Bielecka, Monica Watanabe, Mutsumi Morcuende, Rosa Scheible, Wolf-Rüdiger Hawkesford, Malcolm J. Hesse, Holger Hoefgen, Rainer Biotechnology and Biological Sciences Research Council (UK) Max Planck Society Sulfur Sulfate starvation Nitrate Phosphate Transcription factor Microarray Metabolomics Transcriptomics 18 páginas, 7 figuras, 1 tabla Sulfur is an essential macronutrient for plant growth and development. Reaching a thorough understanding of the molecular basis for changes in plant metabolism depending on the sulfur-nutritional status at the systems level will advance our basic knowledge and help target future crop improvement. Although the transcriptional responses induced by sulfate starvation have been studied in the past, knowledge of the regulation of sulfur metabolism is still fragmentary. This work focuses on the discovery of candidates for regulatory genes such as transcription factors /TFs) using 'omics technologies. For this purpose a short term sulfate-starvation/re-supply approach was used. ATH1 microarray studies and metabolite determinations yialded 21 TFs which responded more than 2-fold at the transcriptional level to sulfate starvation. Categorization by response behaviors under sulfate-starvation/re-supply and other nutrient starvations such as nitrate and phosphate allowed determination of whether the TF genes are specific for or common between distinct mineral nutrient depletions. Extending this co-behavior analysis to the whole transcriptome data set enabled prediction of putative downstream genes. Addinationally, combinations of transcriptome ans metabolome data allowed identification of relationships between TFs and downstream responses, namely, expression changes in biosynthetic genes and subsequent metabolic respnoses. Effect chains on glucosinolate and polyamine biosynthesis are discussed in detail. The knowledge gained fron tis study provides a blueprint for an integrated analysis of trancriptomics and metabolomics and application for the identification of uncharacterized genes. Work at Rothamsted Research is supported via the 20:20 Wheat ® Programme by the UK Biotechnology and Biological Sciences Research Council. Funding, the Max Planck Society (MPG) Peer reviewed 2016-05-12T08:48:21Z 2016-05-12T08:48:21Z 2015 artículo http://purl.org/coar/resource_type/c_6501 Frontiers in Plant Science 5: 1-18 (2015) http://hdl.handle.net/10261/132042 10.3389/fpls.2014.00805 1664-462X http://dx.doi.org/10.13039/501100000268 http://dx.doi.org/10.13039/501100004189 25674096 en Publisher's version http://dx.doi.org/10.3389/fpls.2014.00805 Sí open Frontiers Media |
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| topic |
Sulfur Sulfate starvation Nitrate Phosphate Transcription factor Microarray Metabolomics Transcriptomics Sulfur Sulfate starvation Nitrate Phosphate Transcription factor Microarray Metabolomics Transcriptomics |
| spellingShingle |
Sulfur Sulfate starvation Nitrate Phosphate Transcription factor Microarray Metabolomics Transcriptomics Sulfur Sulfate starvation Nitrate Phosphate Transcription factor Microarray Metabolomics Transcriptomics Bielecka, Monica Watanabe, Mutsumi Morcuende, Rosa Scheible, Wolf-Rüdiger Hawkesford, Malcolm J. Hesse, Holger Hoefgen, Rainer Transcriptome and metabolome analysis of plant sulfate starvation and resupply provides novel information on transcriptional regulation of metabolism associated with sulfur, nitrogen and phosphorus nutritional responses in Arabidopsis |
| description |
18 páginas, 7 figuras, 1 tabla |
| author2 |
Biotechnology and Biological Sciences Research Council (UK) |
| author_facet |
Biotechnology and Biological Sciences Research Council (UK) Bielecka, Monica Watanabe, Mutsumi Morcuende, Rosa Scheible, Wolf-Rüdiger Hawkesford, Malcolm J. Hesse, Holger Hoefgen, Rainer |
| format |
artículo |
| topic_facet |
Sulfur Sulfate starvation Nitrate Phosphate Transcription factor Microarray Metabolomics Transcriptomics |
| author |
Bielecka, Monica Watanabe, Mutsumi Morcuende, Rosa Scheible, Wolf-Rüdiger Hawkesford, Malcolm J. Hesse, Holger Hoefgen, Rainer |
| author_sort |
Bielecka, Monica |
| title |
Transcriptome and metabolome analysis of plant sulfate starvation and resupply provides novel information on transcriptional regulation of metabolism associated with sulfur, nitrogen and phosphorus nutritional responses in Arabidopsis |
| title_short |
Transcriptome and metabolome analysis of plant sulfate starvation and resupply provides novel information on transcriptional regulation of metabolism associated with sulfur, nitrogen and phosphorus nutritional responses in Arabidopsis |
| title_full |
Transcriptome and metabolome analysis of plant sulfate starvation and resupply provides novel information on transcriptional regulation of metabolism associated with sulfur, nitrogen and phosphorus nutritional responses in Arabidopsis |
| title_fullStr |
Transcriptome and metabolome analysis of plant sulfate starvation and resupply provides novel information on transcriptional regulation of metabolism associated with sulfur, nitrogen and phosphorus nutritional responses in Arabidopsis |
| title_full_unstemmed |
Transcriptome and metabolome analysis of plant sulfate starvation and resupply provides novel information on transcriptional regulation of metabolism associated with sulfur, nitrogen and phosphorus nutritional responses in Arabidopsis |
| title_sort |
transcriptome and metabolome analysis of plant sulfate starvation and resupply provides novel information on transcriptional regulation of metabolism associated with sulfur, nitrogen and phosphorus nutritional responses in arabidopsis |
| publisher |
Frontiers Media |
| publishDate |
2015 |
| url |
http://hdl.handle.net/10261/132042 http://dx.doi.org/10.13039/501100000268 http://dx.doi.org/10.13039/501100004189 |
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