New salivary anti-haemostatics containing protective epitopes from Ornithodoros moubata ticks: Assessment of their individual and combined vaccine efficacy

Díaz Martín, Verónica; Manzano Román, Raúl; Oleaga, Ana; Pérez Sánchez, Ricardo; Ministerio de Ciencia e Innovación (España)

New salivary anti-haemostatics containing protective epitopes from Ornithodoros moubata ticks: Assessment of their individual and combined vaccine efficacy

45 páginas, 6 figuras, 5 tablas. -- The definitive version is available at http://wwww.elsevier.com

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Main Authors:	Díaz Martín, Verónica, Manzano Román, Raúl, Oleaga, Ana, Pérez Sánchez, Ricardo
Other Authors:	Ministerio de Ciencia e Innovación (España)
Format:	artículo biblioteca
Language:	English
Published:	Elsevier
Subjects:	Ornithodoros moubata, Salivary antigens, Vaccine, Recombinant antigens, B-cell epitopes,
Online Access:	http://hdl.handle.net/10261/125403 http://dx.doi.org/10.13039/501100004837
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spelling	dig-irnasa-es-10261-1254032019-05-20T08:30:25Z New salivary anti-haemostatics containing protective epitopes from Ornithodoros moubata ticks: Assessment of their individual and combined vaccine efficacy Díaz Martín, Verónica Manzano Román, Raúl Oleaga, Ana Pérez Sánchez, Ricardo Ministerio de Ciencia e Innovación (España) Ornithodoros moubata Salivary antigens Vaccine Recombinant antigens B-cell epitopes 45 páginas, 6 figuras, 5 tablas. -- The definitive version is available at http://wwww.elsevier.com Ornithodoros moubata is the main vector of the pathogens causing African swine fever and human relapsing fever in Africa. The development of an efficient vaccine against this tick would facilitate its control and the prevention of the diseases it transmits to a considerable extent. Previous efforts to identify vaccine target candidates led us to the discovery of novel salivary proteins that probably act as anti-haemostatics at the host-tick interface, including a secreted phospholipase A2 (PLA2), a 7DB-like protein (7DB-like), a riboprotein 60S L10 (RP-60S), an apyrase (APY), and a new platelet aggregation inhibitor peptide, designated mougrin (MOU). In this work, the corresponding recombinant proteins were expressed in Escherichia coli and their individual vaccine efficacy was tested in rabbit vaccination trials. All of them, except the less immunogenic RP-60S, induced strong humoral responses that reduced tick feeding and survival, providing vaccine efficacies of 44.2%, 43.2% and 27.2%, 19.9% and 17.3% for PLA2, APY, MOU, RP-60S and 7DB-like, respectively. In the case of the more protective recombinant antigens (PLA2, APY and MOU), the immunodominant protective linear B-cell epitopes were identified and their combined vaccine efficacy was tested in a second vaccine trial using different adjuvants. In comparison with the best efficacy of individual antigens, the multicomponent vaccine increased vaccine efficacy by 13.6%, indicating additive protective effects rather than a synergistic effect. Tick saliva inoculated during natural tick-host contacts had a boosting effect on vaccinated animals, increasing specific antibody levels and protection Spanish Ministry of Science and Innovation (Grant no. GL2010-18164). Peer reviewed 2015 artículo http://purl.org/coar/resource_type/c_6501 Veterinary Parasitology 212(3-4): 336-349 (2015) 0304-4017 http://hdl.handle.net/10261/125403 10.1016/j.vetpar.2015.08.005 1873-2550 http://dx.doi.org/10.13039/501100004837 en Postprint http://dx.doi.org/10.1016/j.vetpar.2015.08.005 Sí open Elsevier
institution	IRNASA ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-irnasa-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del IRNASA España
language	English
topic	Ornithodoros moubata Salivary antigens Vaccine Recombinant antigens B-cell epitopes Ornithodoros moubata Salivary antigens Vaccine Recombinant antigens B-cell epitopes
spellingShingle	Ornithodoros moubata Salivary antigens Vaccine Recombinant antigens B-cell epitopes Ornithodoros moubata Salivary antigens Vaccine Recombinant antigens B-cell epitopes Díaz Martín, Verónica Manzano Román, Raúl Oleaga, Ana Pérez Sánchez, Ricardo New salivary anti-haemostatics containing protective epitopes from Ornithodoros moubata ticks: Assessment of their individual and combined vaccine efficacy
description	45 páginas, 6 figuras, 5 tablas. -- The definitive version is available at http://wwww.elsevier.com
author2	Ministerio de Ciencia e Innovación (España)
author_facet	Ministerio de Ciencia e Innovación (España) Díaz Martín, Verónica Manzano Román, Raúl Oleaga, Ana Pérez Sánchez, Ricardo
format	artículo
topic_facet	Ornithodoros moubata Salivary antigens Vaccine Recombinant antigens B-cell epitopes
author	Díaz Martín, Verónica Manzano Román, Raúl Oleaga, Ana Pérez Sánchez, Ricardo
author_sort	Díaz Martín, Verónica
title	New salivary anti-haemostatics containing protective epitopes from Ornithodoros moubata ticks: Assessment of their individual and combined vaccine efficacy
title_short	New salivary anti-haemostatics containing protective epitopes from Ornithodoros moubata ticks: Assessment of their individual and combined vaccine efficacy
title_full	New salivary anti-haemostatics containing protective epitopes from Ornithodoros moubata ticks: Assessment of their individual and combined vaccine efficacy
title_fullStr	New salivary anti-haemostatics containing protective epitopes from Ornithodoros moubata ticks: Assessment of their individual and combined vaccine efficacy
title_full_unstemmed	New salivary anti-haemostatics containing protective epitopes from Ornithodoros moubata ticks: Assessment of their individual and combined vaccine efficacy
title_sort	new salivary anti-haemostatics containing protective epitopes from ornithodoros moubata ticks: assessment of their individual and combined vaccine efficacy
publisher	Elsevier
url	http://hdl.handle.net/10261/125403 http://dx.doi.org/10.13039/501100004837
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New salivary anti-haemostatics containing protective epitopes from Ornithodoros moubata ticks: Assessment of their individual and combined vaccine efficacy

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