Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations
In conventional photosystem II preparation high amounts of Cu are found. After fractionation by centrifugation, Cu can be completely removed from photosystem II without affecting either its photosynthetic activity or the composition of its specific proteins. We could demonstrate that the Cu was associated with nuclear contaminants in the starch fraction. Among the contaminants, several histones were identified by specific antisera and by N-terminal sequencing. In order to obtain homogeneous BBY preparations of PSII a procedure is employed that involves a 10000 g centrifugation step and which eliminates non-specifically bound metals, nucleic acids and histones with the starch pellet. The resulting starch-free BBY (BBYs-), which is free of these nuclear contaminants, is an appropriate preparation for biophysical studies or for those of metal interactions with PSII.
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Format: | artículo biblioteca |
Language: | English |
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John Wiley & Sons
1994-07
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Subjects: | Photosystem II, Nuclear contaminants, Copper, Histones, |
Online Access: | http://hdl.handle.net/10261/11101 |
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dig-irnasa-es-10261-111012022-10-06T10:52:08Z Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations Arellano, Juan B. Schröder, Wolfgang P. Sandmann, Gerhard Chueca, Ana Barón Ayala, Matilde Photosystem II Nuclear contaminants Copper Histones In conventional photosystem II preparation high amounts of Cu are found. After fractionation by centrifugation, Cu can be completely removed from photosystem II without affecting either its photosynthetic activity or the composition of its specific proteins. We could demonstrate that the Cu was associated with nuclear contaminants in the starch fraction. Among the contaminants, several histones were identified by specific antisera and by N-terminal sequencing. In order to obtain homogeneous BBY preparations of PSII a procedure is employed that involves a 10000 g centrifugation step and which eliminates non-specifically bound metals, nucleic acids and histones with the starch pellet. The resulting starch-free BBY (BBYs-), which is free of these nuclear contaminants, is an appropriate preparation for biophysical studies or for those of metal interactions with PSII. This work was supported by a grant from the Spanish DGICYT (Project PB 88-0092) and a Spanish-German Exchange Program (MEC-DAAD). J. B. Arellano was the recipient of a predoctoral fellowship from the Spanish Science and Education Ministry. Thanks are due to Junta de Andalucía and the Margit and Foike Pehrzon Foundation for financing W. P. Schroder's and J. B. Arellano's stays in Granada and Stockholm, respectively. Peer reviewed 2009-02-27T15:37:41Z 2009-02-27T15:37:41Z 1994-07 artículo http://purl.org/coar/resource_type/c_6501 Physiol. Plant. (1994) 91:369-374. http://hdl.handle.net/10261/11101 10.1111/j.1399-3054.1994.tb02962.x en http://www3.interscience.wiley.com/journal/119267376/abstract open 635245 bytes application/pdf John Wiley & Sons |
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Photosystem II Nuclear contaminants Copper Histones Photosystem II Nuclear contaminants Copper Histones |
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Photosystem II Nuclear contaminants Copper Histones Photosystem II Nuclear contaminants Copper Histones Arellano, Juan B. Schröder, Wolfgang P. Sandmann, Gerhard Chueca, Ana Barón Ayala, Matilde Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations |
description |
In conventional photosystem II preparation high amounts of Cu are found. After fractionation by centrifugation, Cu can be completely removed from photosystem II without affecting either its photosynthetic activity or the composition of its specific proteins. We could demonstrate that the Cu was associated with nuclear contaminants in the starch fraction. Among the contaminants, several histones were identified by specific antisera and by N-terminal sequencing. In order to obtain homogeneous BBY preparations of PSII a procedure is employed that involves a 10000 g centrifugation step and which eliminates non-specifically bound metals, nucleic acids and histones with the starch pellet. The resulting starch-free BBY (BBYs-), which is free of these nuclear contaminants, is an appropriate preparation for biophysical studies or for those of metal interactions with PSII. |
format |
artículo |
topic_facet |
Photosystem II Nuclear contaminants Copper Histones |
author |
Arellano, Juan B. Schröder, Wolfgang P. Sandmann, Gerhard Chueca, Ana Barón Ayala, Matilde |
author_facet |
Arellano, Juan B. Schröder, Wolfgang P. Sandmann, Gerhard Chueca, Ana Barón Ayala, Matilde |
author_sort |
Arellano, Juan B. |
title |
Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations |
title_short |
Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations |
title_full |
Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations |
title_fullStr |
Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations |
title_full_unstemmed |
Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations |
title_sort |
removal of nuclear contaminants and of non-specifically photosystem ii-bound copper from photosystem ii preparations |
publisher |
John Wiley & Sons |
publishDate |
1994-07 |
url |
http://hdl.handle.net/10261/11101 |
work_keys_str_mv |
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