Structural characterization of the lignin in the cortex and pith of elephant grass (Pennisetum purpureum) stems
The structure of the lignin in the cortex and pith of elephant grass (Pennisetum purpureum) stems were studied both in situ and in isolated milled ‘wood’ lignins by several analytical methods. The presence of p-coumarate and ferulate in the cortex and pith, as well as in their isolated lignins, was revealed by pyrolysis in the presence of TMAH, and by 2D-NMR, and indicated that ferulate acylates the carbohydrates while p-coumarate acylates the lignin polymer. 2D-NMR showed a predominance of β–O–4alkyl aryl ether linkages (82% of total inter-unit linkages), with low amounts of ‘condensed’ substructures, such as resinols, phenylcoumarans, and spirodienones. Moreover, the NMR also indicated that these lignins are extensively acylated at the γ-carbon of the side-chain. DFRC analyses confirmed that p-coumarate groups acylate the γ-OHs of these lignins, and predominantly on syringyl units.
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Format: | capítulo de libro biblioteca |
Language: | English |
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American Chemical Society
2012-08-27
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Subjects: | Elephant grass, Lignin, p-Coumarates, Ferulates, |
Online Access: | http://hdl.handle.net/10261/86339 |
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dig-irnas-es-10261-863392022-04-07T11:26:20Z Structural characterization of the lignin in the cortex and pith of elephant grass (Pennisetum purpureum) stems Río Andrade, José Carlos del Prinsen, Pepijn Rencoret, Jorge Nieto, Lidia Jiménez-Barbero, Jesús Ralph, John Martínez, Ángel T. Gutiérrez Suárez, Ana Elephant grass Lignin p-Coumarates Ferulates The structure of the lignin in the cortex and pith of elephant grass (Pennisetum purpureum) stems were studied both in situ and in isolated milled ‘wood’ lignins by several analytical methods. The presence of p-coumarate and ferulate in the cortex and pith, as well as in their isolated lignins, was revealed by pyrolysis in the presence of TMAH, and by 2D-NMR, and indicated that ferulate acylates the carbohydrates while p-coumarate acylates the lignin polymer. 2D-NMR showed a predominance of β–O–4alkyl aryl ether linkages (82% of total inter-unit linkages), with low amounts of ‘condensed’ substructures, such as resinols, phenylcoumarans, and spirodienones. Moreover, the NMR also indicated that these lignins are extensively acylated at the γ-carbon of the side-chain. DFRC analyses confirmed that p-coumarate groups acylate the γ-OHs of these lignins, and predominantly on syringyl units. This study has been funded by the Spanish project AGL2008-00709, the CSIC project 201040E075 and the EU-project LIGNODECO (KBBE-244362). Prof. John Ralph was funded in part by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-FC02-07ER64494). Peer reviewed 2013-11-12T10:43:25Z 2013-11-12T10:43:25Z 2012-08-27 capítulo de libro http://purl.org/coar/resource_type/c_3248 In: 12th European Workshop on Lignocellulosics and Pulp - EWLP 2012 - Proceedings, pp. 488-491. 978-952-10-8187-3 http://hdl.handle.net/10261/86339 en open American Chemical Society |
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Elephant grass Lignin p-Coumarates Ferulates Elephant grass Lignin p-Coumarates Ferulates |
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Elephant grass Lignin p-Coumarates Ferulates Elephant grass Lignin p-Coumarates Ferulates Río Andrade, José Carlos del Prinsen, Pepijn Rencoret, Jorge Nieto, Lidia Jiménez-Barbero, Jesús Ralph, John Martínez, Ángel T. Gutiérrez Suárez, Ana Structural characterization of the lignin in the cortex and pith of elephant grass (Pennisetum purpureum) stems |
description |
The structure of the lignin in the cortex and pith of elephant grass (Pennisetum purpureum) stems were studied both in situ and in isolated milled ‘wood’ lignins by several analytical methods. The presence of p-coumarate and ferulate in the cortex and pith, as well as in their isolated lignins, was revealed by pyrolysis in the presence of TMAH, and by 2D-NMR, and indicated that ferulate acylates the carbohydrates while p-coumarate acylates the lignin polymer. 2D-NMR showed a predominance of β–O–4alkyl aryl ether linkages (82% of total inter-unit linkages), with low amounts of ‘condensed’ substructures, such as resinols, phenylcoumarans, and spirodienones. Moreover, the NMR also indicated that these lignins are extensively acylated at the γ-carbon of the side-chain. DFRC analyses confirmed that p-coumarate groups acylate the γ-OHs of these lignins, and predominantly on syringyl units. |
format |
capítulo de libro |
topic_facet |
Elephant grass Lignin p-Coumarates Ferulates |
author |
Río Andrade, José Carlos del Prinsen, Pepijn Rencoret, Jorge Nieto, Lidia Jiménez-Barbero, Jesús Ralph, John Martínez, Ángel T. Gutiérrez Suárez, Ana |
author_facet |
Río Andrade, José Carlos del Prinsen, Pepijn Rencoret, Jorge Nieto, Lidia Jiménez-Barbero, Jesús Ralph, John Martínez, Ángel T. Gutiérrez Suárez, Ana |
author_sort |
Río Andrade, José Carlos del |
title |
Structural characterization of the lignin in the cortex and pith of elephant grass (Pennisetum purpureum) stems |
title_short |
Structural characterization of the lignin in the cortex and pith of elephant grass (Pennisetum purpureum) stems |
title_full |
Structural characterization of the lignin in the cortex and pith of elephant grass (Pennisetum purpureum) stems |
title_fullStr |
Structural characterization of the lignin in the cortex and pith of elephant grass (Pennisetum purpureum) stems |
title_full_unstemmed |
Structural characterization of the lignin in the cortex and pith of elephant grass (Pennisetum purpureum) stems |
title_sort |
structural characterization of the lignin in the cortex and pith of elephant grass (pennisetum purpureum) stems |
publisher |
American Chemical Society |
publishDate |
2012-08-27 |
url |
http://hdl.handle.net/10261/86339 |
work_keys_str_mv |
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