Changes in the structure and protein binding ability of condensed tannins during decomposition of fresh needles and leaves

Changes in the structure and protein binding ability of condensed tannins during decomposition of fresh needles and leaves

13 pages, 7 figures, 5 tables, 48 references.

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Bibliographic Details
Main Authors: Maie, Nagamitsu, Behrens, A., Knicker, Heike, Kögel-Knabner, I.
Format: artículo biblioteca
Language:English
Published: Elsevier 2003-04
Subjects:13C NMR, Condensed tannins, Decomposition, MALDI-TOF MS, Proanthocyanidin, Protein binding capacity,
Online Access:http://hdl.handle.net/10261/82255
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spelling dig-irnas-es-10261-822552018-09-12T11:48:57Z Changes in the structure and protein binding ability of condensed tannins during decomposition of fresh needles and leaves Maie, Nagamitsu Behrens, A. Knicker, Heike Kögel-Knabner, I. 13C NMR Condensed tannins Decomposition MALDI-TOF MS Proanthocyanidin Protein binding capacity 13 pages, 7 figures, 5 tables, 48 references. Alterations of the chemical structure of condensed tannins (CT) during decomposition of Norway spruce (Picea abies) needles and white willow (Salix alba) leaves were investigated by gel permeation chromatography (GPC), 13C nuclear magnetic resonance (NMR) spectroscopy, and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry (MS). The effect of these alterations on their protein binding capacity was determined by radial diffusion assay on an agarose plate. For these studies an incubation experiment was performed with spruce needles and willow leaves. From the fresh foliage and its decomposed materials obtained after 4-days, and 1-, 2-, 4-, and 8-weeks of incubation, CT were extracted and analyzed. Dynamics of CT alterations during decomposition of the fresh foliage were different for the two plant species, although the amount of extractable CT for both decreased soon after incubation and only slight amounts of CT were extractable after 8 weeks of incubation. The decrease was faster for the willow leaves than for the spruce needles. Solution 13C NMR revealed alterations of CT isolated from both degrading plant materials, but only to a small extent. However, considerable changes in chemical composition and chain length of the CT were detected by MALDI-TOF MS. Changes in the chemical composition of CT are expected to decrease the protein binding capacity. Applying the radial diffusion assay, this assumption was confirmed for spruce CT, but not for willow CT. This may be explained by (1) higher reactivity of prodelphinidin (PD) than procyanidin (PC), the former is contained in spruce CT but not in willow CT and (2) the slower decomposition rate of spruce needles than willow leaves. Thus, CT in spruce needles may have suffered a greater extent of chemical alteration, which formed partially altered CT with less protein binding capacity. Further, since the decomposition rate of spruce needles was slower than that of willow leaves, the partially altered CT remained extractable for a longer incubation period compared with the willow leave's CT. Peer reviewed 2013-09-18T07:35:58Z 2013-09-18T07:35:58Z 2003-04 artículo http://purl.org/coar/resource_type/c_6501 Soil Biology and Biochemistry: 35 (4): 577-589 (2003) 0038-0717 http://hdl.handle.net/10261/82255 10.1016/S0038-0717(03)00051-8 en http://dx.doi.org/10.1016/S0038-0717(03)00051-8 none Elsevier
institution IRNAS ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-irnas-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IRNAS España
language English
topic 13C NMR
Condensed tannins
Decomposition
MALDI-TOF MS
Proanthocyanidin
Protein binding capacity
13C NMR
Condensed tannins
Decomposition
MALDI-TOF MS
Proanthocyanidin
Protein binding capacity
spellingShingle 13C NMR
Condensed tannins
Decomposition
MALDI-TOF MS
Proanthocyanidin
Protein binding capacity
13C NMR
Condensed tannins
Decomposition
MALDI-TOF MS
Proanthocyanidin
Protein binding capacity
Maie, Nagamitsu
Behrens, A.
Knicker, Heike
Kögel-Knabner, I.
Changes in the structure and protein binding ability of condensed tannins during decomposition of fresh needles and leaves
description 13 pages, 7 figures, 5 tables, 48 references.
format artículo
topic_facet 13C NMR
Condensed tannins
Decomposition
MALDI-TOF MS
Proanthocyanidin
Protein binding capacity
author Maie, Nagamitsu
Behrens, A.
Knicker, Heike
Kögel-Knabner, I.
author_facet Maie, Nagamitsu
Behrens, A.
Knicker, Heike
Kögel-Knabner, I.
author_sort Maie, Nagamitsu
title Changes in the structure and protein binding ability of condensed tannins during decomposition of fresh needles and leaves
title_short Changes in the structure and protein binding ability of condensed tannins during decomposition of fresh needles and leaves
title_full Changes in the structure and protein binding ability of condensed tannins during decomposition of fresh needles and leaves
title_fullStr Changes in the structure and protein binding ability of condensed tannins during decomposition of fresh needles and leaves
title_full_unstemmed Changes in the structure and protein binding ability of condensed tannins during decomposition of fresh needles and leaves
title_sort changes in the structure and protein binding ability of condensed tannins during decomposition of fresh needles and leaves
publisher Elsevier
publishDate 2003-04
url http://hdl.handle.net/10261/82255
work_keys_str_mv AT maienagamitsu changesinthestructureandproteinbindingabilityofcondensedtanninsduringdecompositionoffreshneedlesandleaves
AT behrensa changesinthestructureandproteinbindingabilityofcondensedtanninsduringdecompositionoffreshneedlesandleaves
AT knickerheike changesinthestructureandproteinbindingabilityofcondensedtanninsduringdecompositionoffreshneedlesandleaves
AT kogelknabneri changesinthestructureandproteinbindingabilityofcondensedtanninsduringdecompositionoffreshneedlesandleaves
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