Demonstration of laccase/mediator-based removal of lignin from wood and non-wood feedstocks
9 páginas, 6 figuras, 4 tablas -- PAGS nros. 267-277
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier
2012-09
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| Subjects: | Enzymatic deconstruction, laccase, Bioethanol, lignocellulose, 2D NMR, |
| Online Access: | http://hdl.handle.net/10261/60660 |
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dig-irnas-es-10261-606602020-11-13T13:29:40Z Demonstration of laccase/mediator-based removal of lignin from wood and non-wood feedstocks Gutiérrez Suárez, Ana Rencoret, Jorge Cadena, Edith M. Rico Campos, Alejandro Barth, Dorothee Río Andrade, José Carlos del Martínez, Ángel T. Enzymatic deconstruction laccase Bioethanol lignocellulose 2D NMR 9 páginas, 6 figuras, 4 tablas -- PAGS nros. 267-277 The ability of Trametes villosa laccase, in conjuction with 1-hydroxybenzotriazole (HBT) as mediator and alkaline extraction, to remove lignin was demonstrated during treatment of wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) feedstocks. At 50 U g−1 laccase and 2.5% HBT concentration, 48% and 32% of the Eucalyptus and Pennisetum lignin were removed, respectively. Two-dimensional nuclear magnetic resonance of the feedstocks, swollen in dimethylsulfoxide-d6, revealed the removal of p-hydroxyphenyl, guaiacyl and syringyl lignin units and aliphatic (mainly β-O-4′-linked) side-chains of lignin, and a moderate removal of p-coumaric acid (present in Pennisetum) without a substantial change in polysaccharide cross-signals. The enzymatic pretreatment (at 25 U g−1) of Eucalyptus and Pennisetum feedstocks increased the glucose (by 61% and 12% in 72 h) and ethanol (by 4 and 2 g L−1 in 17 h) yields from both lignocellulosic materials, respectively, as compared to those without enzyme treatment This study was funded by the LIGNODECO EU-project on “Optimised pre-treatment of fast growing woody and nonwoody Brazilian crops by detailed characterization of chemical changes produced in the lignin-carbohydrate matrix” (KBBE-3-244362) and the ELLE, LIGNOCELL and RAPERO Spanish MICINN (co-financed by FEDER funds) projects (AGL2008-00709, AGL2011-25379 and BIO2008-01533, respectively). J. Rencoret acknowledges the CSIC JAE-Doc contract of the program “Junta para la Ampliación de Estudios” co-financed by the European Social Fund (ESF) Peer reviewed 2012-11-20T13:42:16Z 2012-11-20T13:42:16Z 2012-09 artículo http://purl.org/coar/resource_type/c_6501 Bioresource Technology 119:114-122(2012) 0960-8524 http://hdl.handle.net/10261/60660 10.1016/j.biortech.2012.05.112 1873-2976 en http://dx.doi.org/10.1016/j.biortech.2012.05.112 open Elsevier |
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Biblioteca del IRNAS España |
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Enzymatic deconstruction laccase Bioethanol lignocellulose 2D NMR Enzymatic deconstruction laccase Bioethanol lignocellulose 2D NMR |
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Enzymatic deconstruction laccase Bioethanol lignocellulose 2D NMR Enzymatic deconstruction laccase Bioethanol lignocellulose 2D NMR Gutiérrez Suárez, Ana Rencoret, Jorge Cadena, Edith M. Rico Campos, Alejandro Barth, Dorothee Río Andrade, José Carlos del Martínez, Ángel T. Demonstration of laccase/mediator-based removal of lignin from wood and non-wood feedstocks |
| description |
9 páginas, 6 figuras, 4 tablas -- PAGS nros. 267-277 |
| format |
artículo |
| topic_facet |
Enzymatic deconstruction laccase Bioethanol lignocellulose 2D NMR |
| author |
Gutiérrez Suárez, Ana Rencoret, Jorge Cadena, Edith M. Rico Campos, Alejandro Barth, Dorothee Río Andrade, José Carlos del Martínez, Ángel T. |
| author_facet |
Gutiérrez Suárez, Ana Rencoret, Jorge Cadena, Edith M. Rico Campos, Alejandro Barth, Dorothee Río Andrade, José Carlos del Martínez, Ángel T. |
| author_sort |
Gutiérrez Suárez, Ana |
| title |
Demonstration of laccase/mediator-based removal of lignin from wood and non-wood feedstocks |
| title_short |
Demonstration of laccase/mediator-based removal of lignin from wood and non-wood feedstocks |
| title_full |
Demonstration of laccase/mediator-based removal of lignin from wood and non-wood feedstocks |
| title_fullStr |
Demonstration of laccase/mediator-based removal of lignin from wood and non-wood feedstocks |
| title_full_unstemmed |
Demonstration of laccase/mediator-based removal of lignin from wood and non-wood feedstocks |
| title_sort |
demonstration of laccase/mediator-based removal of lignin from wood and non-wood feedstocks |
| publisher |
Elsevier |
| publishDate |
2012-09 |
| url |
http://hdl.handle.net/10261/60660 |
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