Mould and yeast identification in archival settings: preliminary results on the use of traditional methods and molecular biology options in Portuguese archives
This project was developed to fully assess the indoor air quality in archives and libraries from a fungal flora point of view. It uses classical methodologies such as traditional culture media - for the viable fungi - and modern molecular biology protocols, especially relevant to assess the non-viable fraction of the biological contaminants. Denaturing high-performance liquid chromatography (DHPLC) has emerged as an alternative to denaturing gradient gel electrophoresis (DGGE) and has already been applied to the study of a few bacterial communities. We propose the application of DHPLC to the study of fungal colonization on paper-based archive materials. This technology allows for the identification of each component of a mixture of fungi based on their genetic variation. In a highly complex mixture of microbial DNA this method can be used simply to study the population dynamics, and it also allows for sample fraction collection, which can, in many cases, be immediately sequenced, circumventing the need for cloning. Some examples of the methodological application are shown. Also applied is fragment length analysis for the study of mixed Candida samples. Both of these methods can later be applied in various fields, such as clinical and sand sample analysis. So far, the environmental analyses have been extremely useful to determine potentially pathogenic/toxinogenic fungi such as Stachybotrys sp., Aspergillus niger, Aspergillus fumigatus, and Fusarium sp. This work will hopefully lead to more accurate evaluation of environmental conditions for both human health and the preservation of documents.
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2011
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dig-irnas-es-10261-585752018-09-13T09:09:57Z Mould and yeast identification in archival settings: preliminary results on the use of traditional methods and molecular biology options in Portuguese archives Pinheiro, A. C. Macedo, M. F. Jurado, Valme Sáiz-Jiménez, Cesáreo Viegas, C. Brandão, J. Rosado, L. Fundação para a Ciência e a Tecnologia (Portugal) This project was developed to fully assess the indoor air quality in archives and libraries from a fungal flora point of view. It uses classical methodologies such as traditional culture media - for the viable fungi - and modern molecular biology protocols, especially relevant to assess the non-viable fraction of the biological contaminants. Denaturing high-performance liquid chromatography (DHPLC) has emerged as an alternative to denaturing gradient gel electrophoresis (DGGE) and has already been applied to the study of a few bacterial communities. We propose the application of DHPLC to the study of fungal colonization on paper-based archive materials. This technology allows for the identification of each component of a mixture of fungi based on their genetic variation. In a highly complex mixture of microbial DNA this method can be used simply to study the population dynamics, and it also allows for sample fraction collection, which can, in many cases, be immediately sequenced, circumventing the need for cloning. Some examples of the methodological application are shown. Also applied is fragment length analysis for the study of mixed Candida samples. Both of these methods can later be applied in various fields, such as clinical and sand sample analysis. So far, the environmental analyses have been extremely useful to determine potentially pathogenic/toxinogenic fungi such as Stachybotrys sp., Aspergillus niger, Aspergillus fumigatus, and Fusarium sp. This work will hopefully lead to more accurate evaluation of environmental conditions for both human health and the preservation of documents. This work has the financial support of the Fundação para a Ciência e Tecnologia (SFRH/BD/36005/2007). Peer Reviewed 2012-10-22T11:20:14Z 2012-10-22T11:20:14Z 2011 2012-10-22T11:20:14Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.ibiod.2011.02.008 issn: 0964-8305 International Biodeterioration& Biodegradation 65(4): 619- 627 (2011) http://hdl.handle.net/10261/58575 10.1016/j.ibiod.2011.02.008 http://dx.doi.org/10.13039/501100001871 en none Elsevier |
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This project was developed to fully assess the indoor air quality in archives and libraries from a fungal flora point of view. It uses classical methodologies such as traditional culture media - for the viable fungi - and modern molecular biology protocols, especially relevant to assess the non-viable fraction of the biological contaminants. Denaturing high-performance liquid chromatography (DHPLC) has emerged as an alternative to denaturing gradient gel electrophoresis (DGGE) and has already been applied to the study of a few bacterial communities. We propose the application of DHPLC to the study of fungal colonization on paper-based archive materials. This technology allows for the identification of each component of a mixture of fungi based on their genetic variation. In a highly complex mixture of microbial DNA this method can be used simply to study the population dynamics, and it also allows for sample fraction collection, which can, in many cases, be immediately sequenced, circumventing the need for cloning. Some examples of the methodological application are shown. Also applied is fragment length analysis for the study of mixed Candida samples. Both of these methods can later be applied in various fields, such as clinical and sand sample analysis. So far, the environmental analyses have been extremely useful to determine potentially pathogenic/toxinogenic fungi such as Stachybotrys sp., Aspergillus niger, Aspergillus fumigatus, and Fusarium sp. This work will hopefully lead to more accurate evaluation of environmental conditions for both human health and the preservation of documents. |
author2 |
Fundação para a Ciência e a Tecnologia (Portugal) |
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Fundação para a Ciência e a Tecnologia (Portugal) Pinheiro, A. C. Macedo, M. F. Jurado, Valme Sáiz-Jiménez, Cesáreo Viegas, C. Brandão, J. Rosado, L. |
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Pinheiro, A. C. Macedo, M. F. Jurado, Valme Sáiz-Jiménez, Cesáreo Viegas, C. Brandão, J. Rosado, L. |
spellingShingle |
Pinheiro, A. C. Macedo, M. F. Jurado, Valme Sáiz-Jiménez, Cesáreo Viegas, C. Brandão, J. Rosado, L. Mould and yeast identification in archival settings: preliminary results on the use of traditional methods and molecular biology options in Portuguese archives |
author_sort |
Pinheiro, A. C. |
title |
Mould and yeast identification in archival settings: preliminary results on the use of traditional methods and molecular biology options in Portuguese archives |
title_short |
Mould and yeast identification in archival settings: preliminary results on the use of traditional methods and molecular biology options in Portuguese archives |
title_full |
Mould and yeast identification in archival settings: preliminary results on the use of traditional methods and molecular biology options in Portuguese archives |
title_fullStr |
Mould and yeast identification in archival settings: preliminary results on the use of traditional methods and molecular biology options in Portuguese archives |
title_full_unstemmed |
Mould and yeast identification in archival settings: preliminary results on the use of traditional methods and molecular biology options in Portuguese archives |
title_sort |
mould and yeast identification in archival settings: preliminary results on the use of traditional methods and molecular biology options in portuguese archives |
publisher |
Elsevier |
publishDate |
2011 |
url |
http://hdl.handle.net/10261/58575 http://dx.doi.org/10.13039/501100001871 |
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