An efficient strategy for screening large cloned libraries of amplified 16S rDNA sequences from complex environmental communities
5 pages, 3 figures, 23 references.
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| Main Authors: | , , , , |
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| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Elsevier
2003-11
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| Subjects: | Fingerprinting methods, Screening, Microbial diversity, DGGE, Cloning, 16S rDNA, |
| Online Access: | http://hdl.handle.net/10261/56191 http://dx.doi.org/10.13039/501100006280 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100000780 |
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dig-irnas-es-10261-561912018-09-13T09:00:04Z An efficient strategy for screening large cloned libraries of amplified 16S rDNA sequences from complex environmental communities González Grau, Juan Miguel Ortiz Martínez, A. González del Valle, M. A. Laiz Trobajo, L. Sáiz-Jiménez, Cesáreo Ministerio de Ciencia y Tecnología (España) Consejo Superior de Investigaciones Científicas (España) European Commission Fingerprinting methods Screening Microbial diversity DGGE Cloning 16S rDNA 5 pages, 3 figures, 23 references. We propose a strategy for the efficient screening of large libraries of amplified 16S rRNA genes from complex environmental samples. It consists of processing sets of multiple clones simultaneously. This strategy saves up to 90% of the costs and labor spent in the process of screening a 16S rDNA library. JMG thanks funding from the Spanish Ministry of Science and Technology (MCYT), Ramón y Cajal programme. LL thank funding from the CSIC-I3P programme. This study was funded by MCYT pro- jects MAT2000-0261-P4-04, REN2002-0041/GLO, and BTE2002-04492-CO2-01. Peer reviewed 2012-09-12T14:52:53Z 2012-09-12T14:52:53Z 2003-11 artículo http://purl.org/coar/resource_type/c_6501 Journal of Microbiological Methods 55(2): 459-463 (2003) 0167-7012 http://hdl.handle.net/10261/56191 10.1016/S0167-7012(03)00171-4 http://dx.doi.org/10.13039/501100006280 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100000780 en http://dx.doi.org/10.1016/S0167-7012(03)00171-4 none Elsevier |
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| language |
English |
| topic |
Fingerprinting methods Screening Microbial diversity DGGE Cloning 16S rDNA Fingerprinting methods Screening Microbial diversity DGGE Cloning 16S rDNA |
| spellingShingle |
Fingerprinting methods Screening Microbial diversity DGGE Cloning 16S rDNA Fingerprinting methods Screening Microbial diversity DGGE Cloning 16S rDNA González Grau, Juan Miguel Ortiz Martínez, A. González del Valle, M. A. Laiz Trobajo, L. Sáiz-Jiménez, Cesáreo An efficient strategy for screening large cloned libraries of amplified 16S rDNA sequences from complex environmental communities |
| description |
5 pages, 3 figures, 23 references. |
| author2 |
Ministerio de Ciencia y Tecnología (España) |
| author_facet |
Ministerio de Ciencia y Tecnología (España) González Grau, Juan Miguel Ortiz Martínez, A. González del Valle, M. A. Laiz Trobajo, L. Sáiz-Jiménez, Cesáreo |
| format |
artículo |
| topic_facet |
Fingerprinting methods Screening Microbial diversity DGGE Cloning 16S rDNA |
| author |
González Grau, Juan Miguel Ortiz Martínez, A. González del Valle, M. A. Laiz Trobajo, L. Sáiz-Jiménez, Cesáreo |
| author_sort |
González Grau, Juan Miguel |
| title |
An efficient strategy for screening large cloned libraries of amplified 16S rDNA sequences from complex environmental communities |
| title_short |
An efficient strategy for screening large cloned libraries of amplified 16S rDNA sequences from complex environmental communities |
| title_full |
An efficient strategy for screening large cloned libraries of amplified 16S rDNA sequences from complex environmental communities |
| title_fullStr |
An efficient strategy for screening large cloned libraries of amplified 16S rDNA sequences from complex environmental communities |
| title_full_unstemmed |
An efficient strategy for screening large cloned libraries of amplified 16S rDNA sequences from complex environmental communities |
| title_sort |
efficient strategy for screening large cloned libraries of amplified 16s rdna sequences from complex environmental communities |
| publisher |
Elsevier |
| publishDate |
2003-11 |
| url |
http://hdl.handle.net/10261/56191 http://dx.doi.org/10.13039/501100006280 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100000780 |
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