In vitro germplasm conservation of Atropa baetica by cold storage
3 pages, 4 figures, 10 references.
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Royal Botanic Gardens, Kew
1998-10
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| Online Access: | http://hdl.handle.net/10261/47164 http://dx.doi.org/10.13039/501100007273 |
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dig-irnas-es-10261-471642018-09-10T09:54:35Z In vitro germplasm conservation of Atropa baetica by cold storage Cantos, Manuel Zárate, Rafael Troncoso de Arce, Antonio Comisión Interministerial de Ciencia y Tecnología, CICYT (España) 3 pages, 4 figures, 10 references. Germplasm of in vitro derived plantlels of Atropa baetica was maintained by cold storage at 4°C in darkness for six months. The response of the cold stored germplasm to growing culture conditions was stludied on Murashige & Skoog medium supplemented wilh BAP (0.66 mg l-1), NAA (0.24 mg l-1), inositol (100 mg l-1), thiamine (1 mg l-1) and 3% sucrose during a six month period. The survival rate of the germplasm was similar to the control (plant material grown under normal culture conditions), with an average percentage of 97.9 over the six month period. The number of buds produced was similar for both control and cold stored germplasm with an average 4.53 buds per explant during the six month period. Similarly, the number of shoots per explant did not differ between control and treatment. However, shoot length of cold stored germplasm was statistically shorter for the 1st and 5th month although the overall six month measurements showed no difference. This work has been supported by CICYT (Spanish Govcrnmenl), grant number AMB94. 1372. Peer reviewed 2012-03-16T11:04:41Z 2012-03-16T11:04:41Z 1998-10 artículo http://purl.org/coar/resource_type/c_6501 Botanic Gardens Micropropagation News 2(3): 37-39 (1998) 0962-7448 http://hdl.handle.net/10261/47164 http://dx.doi.org/10.13039/501100007273 en open Royal Botanic Gardens, Kew |
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biblioteca |
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| language |
English |
| description |
3 pages, 4 figures, 10 references. |
| author2 |
Comisión Interministerial de Ciencia y Tecnología, CICYT (España) |
| author_facet |
Comisión Interministerial de Ciencia y Tecnología, CICYT (España) Cantos, Manuel Zárate, Rafael Troncoso de Arce, Antonio |
| format |
artículo |
| author |
Cantos, Manuel Zárate, Rafael Troncoso de Arce, Antonio |
| spellingShingle |
Cantos, Manuel Zárate, Rafael Troncoso de Arce, Antonio In vitro germplasm conservation of Atropa baetica by cold storage |
| author_sort |
Cantos, Manuel |
| title |
In vitro germplasm conservation of Atropa baetica by cold storage |
| title_short |
In vitro germplasm conservation of Atropa baetica by cold storage |
| title_full |
In vitro germplasm conservation of Atropa baetica by cold storage |
| title_fullStr |
In vitro germplasm conservation of Atropa baetica by cold storage |
| title_full_unstemmed |
In vitro germplasm conservation of Atropa baetica by cold storage |
| title_sort |
in vitro germplasm conservation of atropa baetica by cold storage |
| publisher |
Royal Botanic Gardens, Kew |
| publishDate |
1998-10 |
| url |
http://hdl.handle.net/10261/47164 http://dx.doi.org/10.13039/501100007273 |
| work_keys_str_mv |
AT cantosmanuel invitrogermplasmconservationofatropabaeticabycoldstorage AT zaraterafael invitrogermplasmconservationofatropabaeticabycoldstorage AT troncosodearceantonio invitrogermplasmconservationofatropabaeticabycoldstorage |
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