Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis

Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis

4 pages, 2 tables, 2 figures, 29 references. We acknowledge Aguas de Jerez E.M.S.A. for the use of the Guadalete wastewater treatment plant and for technical assistance and E. Iglesias from Yara Iberian for providing Nutriox.

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Bibliographic Details
Main Authors: Portillo Guisado, María del Carmen, Villahermosa, Desiré, Corzo Rodríguez, Alfonso, González Grau, Juan Miguel
Other Authors: Junta de Andalucía
Format: artículo biblioteca
Language:English
Published: American Society for Microbiology 2011-01
Subjects:Microbial community, Fingerprinting, DGGE, DD-DGGE, Differential display, Wastewater,
Online Access:http://hdl.handle.net/10261/40398
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100011011
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spelling dig-irnas-es-10261-403982018-09-11T10:24:54Z Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis Portillo Guisado, María del Carmen Villahermosa, Desiré Corzo Rodríguez, Alfonso González Grau, Juan Miguel Junta de Andalucía Ministerio de Ciencia e Innovación (España) Microbial community Fingerprinting DGGE, DD-DGGE Differential display Wastewater 4 pages, 2 tables, 2 figures, 29 references. We acknowledge Aguas de Jerez E.M.S.A. for the use of the Guadalete wastewater treatment plant and for technical assistance and E. Iglesias from Yara Iberian for providing Nutriox. Complex microbial communities exhibit a large diversity, hampering differentiation by DNA fingerprinting. Herein, differential display-denaturing gradient gel electrophoresis is proposed. By adding a nucleotide to the 3'ends of PCR primers, 16 primer pairs and fingerprints were generated per community. Complexity reduction in each partial fingerprint facilitates sample comparison. This work was supported mainly by grant P06-RNM-01787 from the Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía, Spain, and by projects CGL2009-12328/BOS and Consolider CSD2009-00006 from the Spanish Ministry of Science and Innovation. Peer reviewed 2011-09-30T11:45:32Z 2011-09-30T11:45:32Z 2011-01 artículo http://purl.org/coar/resource_type/c_6501 Applied and Environmental Microbiology 77(1): 351-354 (2011) 0099-2240 http://hdl.handle.net/10261/40398 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011011 en http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3019698/ doi:10.1128/AEM.01316-10 open American Society for Microbiology
institution IRNAS ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-irnas-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IRNAS España
language English
topic Microbial community
Fingerprinting
DGGE, DD-DGGE
Differential display
Wastewater
Microbial community
Fingerprinting
DGGE, DD-DGGE
Differential display
Wastewater
spellingShingle Microbial community
Fingerprinting
DGGE, DD-DGGE
Differential display
Wastewater
Microbial community
Fingerprinting
DGGE, DD-DGGE
Differential display
Wastewater
Portillo Guisado, María del Carmen
Villahermosa, Desiré
Corzo Rodríguez, Alfonso
González Grau, Juan Miguel
Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis
description 4 pages, 2 tables, 2 figures, 29 references. We acknowledge Aguas de Jerez E.M.S.A. for the use of the Guadalete wastewater treatment plant and for technical assistance and E. Iglesias from Yara Iberian for providing Nutriox.
author2 Junta de Andalucía
author_facet Junta de Andalucía
Portillo Guisado, María del Carmen
Villahermosa, Desiré
Corzo Rodríguez, Alfonso
González Grau, Juan Miguel
format artículo
topic_facet Microbial community
Fingerprinting
DGGE, DD-DGGE
Differential display
Wastewater
author Portillo Guisado, María del Carmen
Villahermosa, Desiré
Corzo Rodríguez, Alfonso
González Grau, Juan Miguel
author_sort Portillo Guisado, María del Carmen
title Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis
title_short Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis
title_full Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis
title_fullStr Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis
title_full_unstemmed Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis
title_sort microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis
publisher American Society for Microbiology
publishDate 2011-01
url http://hdl.handle.net/10261/40398
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100011011
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AT villahermosadesire microbialcommunityfingerprintingbydifferentialdisplaydenaturinggradientgelelectrophoresis
AT corzorodriguezalfonso microbialcommunityfingerprintingbydifferentialdisplaydenaturinggradientgelelectrophoresis
AT gonzalezgraujuanmiguel microbialcommunityfingerprintingbydifferentialdisplaydenaturinggradientgelelectrophoresis
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