The AtNHX1 exchanger mediates potassium compartmentation in vacuoles of transgenic tomato
Ledidi, Eduardo O. et al.-- 12 páginas, 6 figuras, 3 tablas.
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| Format: | artículo biblioteca |
| Language: | English |
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Wiley-Blackwell
2010-02
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| Subjects: | NA+/H+ Antiporter Gene, Confers salt tolerance, CV heavenly-blue, Zostera-Marina L., Plasma-membrane, Arabidopsis thaliana, Morning glory, Physiological evidence, Flower coloration, Ion homeostasis, |
| Online Access: | http://hdl.handle.net/10261/37780 http://dx.doi.org/10.13039/501100003141 http://dx.doi.org/10.13039/501100011011 |
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dig-irnas-es-10261-377802019-11-14T12:45:38Z The AtNHX1 exchanger mediates potassium compartmentation in vacuoles of transgenic tomato Leidi, Eduardo O. Pardo, José M. Barragán, Verónica El Hamdaoui, Addelaziz Ruiz, Mª Teresa Cubero, Beatriz Quintero, Francisco J. Ministerio de Educación y Ciencia (España) Junta de Andalucía Consejo Nacional de Ciencia y Tecnología (México) NA+/H+ Antiporter Gene Confers salt tolerance CV heavenly-blue Zostera-Marina L. Plasma-membrane Arabidopsis thaliana Morning glory Physiological evidence Flower coloration Ion homeostasis Ledidi, Eduardo O. et al.-- 12 páginas, 6 figuras, 3 tablas. NHX-type antiporters in the tonoplast have been reported to increase the salt tolerance of various plants species, and are thought to mediate the compartmentation of Na+ in vacuoles. However, all isoforms characterized so far catalyze both Na+/H+ and K+/H+ exchange. Here, we show that AtNHX1 has a critical involvement in the subcellular partitioning of K+, which in turn affects plant K+ nutrition and Na+ tolerance. Transgenic tomato plants overexpressing AtNHX1 had larger K+ vacuolar pools in all growth conditions tested, but no consistent enhancement of Na+ accumulation was observed under salt stress. Plants overexpressing AtNHX1 have a greater capacity to retain intracellular K+ and to withstand salt-shock. Under K+-limiting conditions, greater K+ compartmentation in the vacuole occurred at the expense of the cytosolic K+ pool, which was lower in transgenic plants. This caused the early activation of the high-affinity K+ uptake system, enhanced K+ uptake by roots, and increased the K+ content in plant tissues and the xylem sap of transformed plants. Our results strongly suggest that NHX proteins are likely candidates for the H+-linked K+ transport that is thought to facilitate active K+ uptake at the tonoplast, and the partitioning of K+ between vacuole and cytosol. This work was supported by grants BFU2006-06968 from Ministerio de Educación y Ciencia and AGR-1482 from Junta de Andalucíia to JMP. Additional support was obtained from CSIC-CONACYT Bilateral Cooperation Grant 2004MX0021. Peer reviewed 2011-07-14T15:28:30Z 2011-07-14T15:28:30Z 2010-02 artículo http://purl.org/coar/resource_type/c_6501 The Plant Journal 61(3): 495-506 (2010) 0960-7412 http://hdl.handle.net/10261/37780 10.1111/j.1365-313X.2009.04073.x 1365-313X http://dx.doi.org/10.13039/501100003141 http://dx.doi.org/10.13039/501100011011 en http://dx.doi.org/10.1111/j.1365-313X.2009.04073.x open Wiley-Blackwell |
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NA+/H+ Antiporter Gene Confers salt tolerance CV heavenly-blue Zostera-Marina L. Plasma-membrane Arabidopsis thaliana Morning glory Physiological evidence Flower coloration Ion homeostasis NA+/H+ Antiporter Gene Confers salt tolerance CV heavenly-blue Zostera-Marina L. Plasma-membrane Arabidopsis thaliana Morning glory Physiological evidence Flower coloration Ion homeostasis |
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NA+/H+ Antiporter Gene Confers salt tolerance CV heavenly-blue Zostera-Marina L. Plasma-membrane Arabidopsis thaliana Morning glory Physiological evidence Flower coloration Ion homeostasis NA+/H+ Antiporter Gene Confers salt tolerance CV heavenly-blue Zostera-Marina L. Plasma-membrane Arabidopsis thaliana Morning glory Physiological evidence Flower coloration Ion homeostasis Leidi, Eduardo O. Pardo, José M. Barragán, Verónica El Hamdaoui, Addelaziz Ruiz, Mª Teresa Cubero, Beatriz Quintero, Francisco J. The AtNHX1 exchanger mediates potassium compartmentation in vacuoles of transgenic tomato |
| description |
Ledidi, Eduardo O. et al.-- 12 páginas, 6 figuras, 3 tablas. |
| author2 |
Ministerio de Educación y Ciencia (España) |
| author_facet |
Ministerio de Educación y Ciencia (España) Leidi, Eduardo O. Pardo, José M. Barragán, Verónica El Hamdaoui, Addelaziz Ruiz, Mª Teresa Cubero, Beatriz Quintero, Francisco J. |
| format |
artículo |
| topic_facet |
NA+/H+ Antiporter Gene Confers salt tolerance CV heavenly-blue Zostera-Marina L. Plasma-membrane Arabidopsis thaliana Morning glory Physiological evidence Flower coloration Ion homeostasis |
| author |
Leidi, Eduardo O. Pardo, José M. Barragán, Verónica El Hamdaoui, Addelaziz Ruiz, Mª Teresa Cubero, Beatriz Quintero, Francisco J. |
| author_sort |
Leidi, Eduardo O. |
| title |
The AtNHX1 exchanger mediates potassium compartmentation in vacuoles of transgenic tomato |
| title_short |
The AtNHX1 exchanger mediates potassium compartmentation in vacuoles of transgenic tomato |
| title_full |
The AtNHX1 exchanger mediates potassium compartmentation in vacuoles of transgenic tomato |
| title_fullStr |
The AtNHX1 exchanger mediates potassium compartmentation in vacuoles of transgenic tomato |
| title_full_unstemmed |
The AtNHX1 exchanger mediates potassium compartmentation in vacuoles of transgenic tomato |
| title_sort |
atnhx1 exchanger mediates potassium compartmentation in vacuoles of transgenic tomato |
| publisher |
Wiley-Blackwell |
| publishDate |
2010-02 |
| url |
http://hdl.handle.net/10261/37780 http://dx.doi.org/10.13039/501100003141 http://dx.doi.org/10.13039/501100011011 |
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