Identification of Potential Antimicrobial Compounds from a Marine Streptomyces sp. SM2.4 Strain (MH752437) Isolated from Rachgoun Island in Western Algeria
14 páginas.- 4 figuras.- 8 tablas.- 60 referencias
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Bentham Science Publishers
2021-02-22
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| Subjects: | 16S rRNA gene, Antimicrobial activity, Bioactive compounds, Crude extract, GC/MS, Marine actinomycetes, |
| Online Access: | http://hdl.handle.net/10261/276378 |
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dig-irnas-es-10261-2763782022-11-07T17:01:46Z Identification of Potential Antimicrobial Compounds from a Marine Streptomyces sp. SM2.4 Strain (MH752437) Isolated from Rachgoun Island in Western Algeria Boublenza, Nesrine Dergal, Nadir Boudjlal Belyagoubi, Larbi Gabed, Noujoud Abdelouahid, Djamel E. Redissi. Alaeddine Cherif, Ameur Mosbahm Amor Jurado, Valme Sáiz-Jiménez, Cesáreo El-Amine Abi-Ayad, Sidi-M. Dergal, Nadir Boudjlal [0000-0002-0945-2930] Jurado, Valme [0000-0003-0972-9909] Sáiz-Jiménez, Cesáreo [0000-0003-0036-670X] 16S rRNA gene Antimicrobial activity Bioactive compounds Crude extract GC/MS Marine actinomycetes 14 páginas.- 4 figuras.- 8 tablas.- 60 referencias Background: Marine actinobacteria are a potential resource for natural products; their secondary bioactive metabolites have shown several biological activities. Most of the isolated and identified actinobacteria in Algeria were usually explored from caves, Saharan soil or palm groves. The marine ecosystem is poorly explored and documented. Methods: Five Streptomyces strains producing bioactive compounds were isolated from Rachgoun Island located in Western Algeria and characterised phenotypically and genotypically using microbiological and 16S rRNA sequencing methods, respectively. The crude extract of the most representative strain ¿Streptomyces sp. strain SM2.4¿ and its seven active fractions were characterised by GC/MS analysis. Results: Streptomyces sp. strain SM2.4 revealed the strongest activity against the Gram-positive bacteria Staphylococcus aureus and Bacillus subtilis, the fungus Aspergillus niger and was inactive against Gram-negative bacteria. GC/MS analysis of the methylated crude extract of Streptomyces sp. strain SM2.4 revealed the presence of 11 major compounds including fatty acids methyl ester (12-methyltridecanoic acid methyl ester, 9-hexadecenoic acid methyl ester, hexadecanoic acid methyl ester, 14-methylhexadecanoic acid methyl ester and 16-methylheptadecanoic acid methyl ester), 2,4-di-tert-butylphenol, (4S,4aS,8aR)-4,8a-dimethyloctahydro-4a(2H)-naphthalenol (geosmin), 2,4-dimethylbenzaldehyde, 3,4-difluorobenzaldehyde, dimethylfuran-2,4-dicarboxylate and pyrrolo(1,2-a)pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-. Partial purification of the crude extract by Thin-layer chromatography provided seven active fractions which were tested by radial diffusion assay. GC/MS analysis of the active TLC-fractions revealed the presence of a mixture of active compounds from which 2-(bromomethyl)-2-(2-methylphenyl)-1,3-dioxolane was found to be a new 1,3 dioxolane derivative. Furthermore, 3,4-dimethylbenzamide and pyrido[2,3-d] pyridazine-1,4-dione, hexahydro-3-(2-methylpropyl)-, were extracted for the first time from a natural source. Conclusion: Our study reveals that marine Streptomyces sp. strain SM2.4 has an interesting antimicrobial potential due to its panel of bioactive compounds. Peer reviewed 2022-08-22T08:30:03Z 2022-08-22T08:30:03Z 2021-02-22 2022-08-22T08:30:04Z artículo doi: 10.2174/1573407217666210223104350 issn: 1573-4072 e-issn: 1875-6646 Current Bioactive Compounds 17(10): e190721191724 (2021) http://hdl.handle.net/10261/276378 10.2174/1573407217666210223104350 http://dx.doi.org/10.2174/1573407217666210223104350 Sí none Bentham Science Publishers |
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16S rRNA gene Antimicrobial activity Bioactive compounds Crude extract GC/MS Marine actinomycetes 16S rRNA gene Antimicrobial activity Bioactive compounds Crude extract GC/MS Marine actinomycetes |
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16S rRNA gene Antimicrobial activity Bioactive compounds Crude extract GC/MS Marine actinomycetes 16S rRNA gene Antimicrobial activity Bioactive compounds Crude extract GC/MS Marine actinomycetes Boublenza, Nesrine Dergal, Nadir Boudjlal Belyagoubi, Larbi Gabed, Noujoud Abdelouahid, Djamel E. Redissi. Alaeddine Cherif, Ameur Mosbahm Amor Jurado, Valme Sáiz-Jiménez, Cesáreo El-Amine Abi-Ayad, Sidi-M. Identification of Potential Antimicrobial Compounds from a Marine Streptomyces sp. SM2.4 Strain (MH752437) Isolated from Rachgoun Island in Western Algeria |
| description |
14 páginas.- 4 figuras.- 8 tablas.- 60 referencias |
| author2 |
Dergal, Nadir Boudjlal [0000-0002-0945-2930] |
| author_facet |
Dergal, Nadir Boudjlal [0000-0002-0945-2930] Boublenza, Nesrine Dergal, Nadir Boudjlal Belyagoubi, Larbi Gabed, Noujoud Abdelouahid, Djamel E. Redissi. Alaeddine Cherif, Ameur Mosbahm Amor Jurado, Valme Sáiz-Jiménez, Cesáreo El-Amine Abi-Ayad, Sidi-M. |
| format |
artículo |
| topic_facet |
16S rRNA gene Antimicrobial activity Bioactive compounds Crude extract GC/MS Marine actinomycetes |
| author |
Boublenza, Nesrine Dergal, Nadir Boudjlal Belyagoubi, Larbi Gabed, Noujoud Abdelouahid, Djamel E. Redissi. Alaeddine Cherif, Ameur Mosbahm Amor Jurado, Valme Sáiz-Jiménez, Cesáreo El-Amine Abi-Ayad, Sidi-M. |
| author_sort |
Boublenza, Nesrine |
| title |
Identification of Potential Antimicrobial Compounds from a Marine Streptomyces sp. SM2.4 Strain (MH752437) Isolated from Rachgoun Island in Western Algeria |
| title_short |
Identification of Potential Antimicrobial Compounds from a Marine Streptomyces sp. SM2.4 Strain (MH752437) Isolated from Rachgoun Island in Western Algeria |
| title_full |
Identification of Potential Antimicrobial Compounds from a Marine Streptomyces sp. SM2.4 Strain (MH752437) Isolated from Rachgoun Island in Western Algeria |
| title_fullStr |
Identification of Potential Antimicrobial Compounds from a Marine Streptomyces sp. SM2.4 Strain (MH752437) Isolated from Rachgoun Island in Western Algeria |
| title_full_unstemmed |
Identification of Potential Antimicrobial Compounds from a Marine Streptomyces sp. SM2.4 Strain (MH752437) Isolated from Rachgoun Island in Western Algeria |
| title_sort |
identification of potential antimicrobial compounds from a marine streptomyces sp. sm2.4 strain (mh752437) isolated from rachgoun island in western algeria |
| publisher |
Bentham Science Publishers |
| publishDate |
2021-02-22 |
| url |
http://hdl.handle.net/10261/276378 |
| work_keys_str_mv |
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