Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives
14 páginas.- 6 figuras. 1 tabla.- 50 referencias.- Supplementary Information Te online version contains supplementary material available at https://doi.org/10.1038/s41598-021-91756-5
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Springer Nature
2021-06-15
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| Online Access: | http://hdl.handle.net/10261/245474 http://dx.doi.org/10.13039/100000015 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100005946 http://dx.doi.org/10.13039/501100001691 http://dx.doi.org/10.13039/100000001 |
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dig-irnas-es-10261-2454742021-07-10T01:14:43Z Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives Iwata, Mana Gutiérrez Suárez, Ana Marques, Gisela Sabat, Grzegorz Kersten, Philip J Cullen, Daniel Bhatnagar, Jennifer M. Yadav, Jagjit S. Lipzen, Anna Yoshinaga, Yuko Sharma, Aditi Adam, Catherine Joint Genome Institute (US) National Science Foundation (US) Department of Energy (US) Japan Society for the Promotion of Science Consejo Superior de Investigaciones Científicas (España) Hokkaido University Gutiérrez Suárez, Ana [0000-0002-8823-9029] Marques, Gisela [0000-0002-6431-8267] Bhatnagar, Jennifer M. [0000-0001-6424-4133] 14 páginas.- 6 figuras. 1 tabla.- 50 referencias.- Supplementary Information Te online version contains supplementary material available at https://doi.org/10.1038/s41598-021-91756-5 Wood extractives, solvent-soluble fractions of woody biomass, are considered to be a factor impeding or excluding fungal colonization on the freshly harvested conifers. Among wood decay fungi, the basidiomycete Phlebiopsis gigantea has evolved a unique enzyme system to efficiently transform or degrade conifer extractives but little is known about the mechanism(s). In this study, to clarify the mechanism(s) of softwood degradation, we examined the transcriptome, proteome, and metabolome of P. gigantea when grown on defined media containing microcrystalline cellulose and pine sapwood extractives. Beyond the conventional enzymes often associated with cellulose, hemicellulose and lignin degradation, an array of enzymes implicated in the metabolism of softwood lipophilic extractives such as fatty and resin acids, steroids and glycerides was significantly up-regulated. Among these, a highly expressed and inducible lipase is likely responsible for lipophilic extractive degradation, based on its extracellular location and our characterization of the recombinant enzyme. Our results provide insight into physiological roles of extractives in the interaction between wood and fungi. © 2021, The Author(s). The work partly conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. Research was also supported by NSF Grants 1457695 and 1457721 to J.M.B. and D.C., respectively, by CSIC project 201740E071 to A.G. and by JSPS Grant-in-Aid for Scientific Research 19K15881, JST-ACTX PJ2519A059 and 2017 Feasibility Study Program of the Frontier Chemistry Center, Faculty of Engineering, Hokkaido University to C.H. Peer reviewed 2021-07-08T09:16:48Z 2021-07-08T09:16:48Z 2021-06-15 artículo http://purl.org/coar/resource_type/c_6501 Scientific Reports 11: 12528 (2021) http://hdl.handle.net/10261/245474 10.1038/s41598-021-91756-5 2045-2322 http://dx.doi.org/10.13039/100000015 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100005946 http://dx.doi.org/10.13039/501100001691 http://dx.doi.org/10.13039/100000001 34131180 en Publisher's version http://dx.doi.org/10.1038/s41598-021-91756-5 Sí open Springer Nature |
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14 páginas.- 6 figuras. 1 tabla.- 50 referencias.- Supplementary Information Te online version contains supplementary material available at https://doi.org/10.1038/s41598-021-91756-5 |
| author2 |
Joint Genome Institute (US) |
| author_facet |
Joint Genome Institute (US) Iwata, Mana Gutiérrez Suárez, Ana Marques, Gisela Sabat, Grzegorz Kersten, Philip J Cullen, Daniel Bhatnagar, Jennifer M. Yadav, Jagjit S. Lipzen, Anna Yoshinaga, Yuko Sharma, Aditi Adam, Catherine |
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artículo |
| author |
Iwata, Mana Gutiérrez Suárez, Ana Marques, Gisela Sabat, Grzegorz Kersten, Philip J Cullen, Daniel Bhatnagar, Jennifer M. Yadav, Jagjit S. Lipzen, Anna Yoshinaga, Yuko Sharma, Aditi Adam, Catherine |
| spellingShingle |
Iwata, Mana Gutiérrez Suárez, Ana Marques, Gisela Sabat, Grzegorz Kersten, Philip J Cullen, Daniel Bhatnagar, Jennifer M. Yadav, Jagjit S. Lipzen, Anna Yoshinaga, Yuko Sharma, Aditi Adam, Catherine Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives |
| author_sort |
Iwata, Mana |
| title |
Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives |
| title_short |
Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives |
| title_full |
Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives |
| title_fullStr |
Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives |
| title_full_unstemmed |
Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives |
| title_sort |
omics analyses and biochemical study of phlebiopsis gigantea elucidate its degradation strategy of wood extractives |
| publisher |
Springer Nature |
| publishDate |
2021-06-15 |
| url |
http://hdl.handle.net/10261/245474 http://dx.doi.org/10.13039/100000015 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100005946 http://dx.doi.org/10.13039/501100001691 http://dx.doi.org/10.13039/100000001 |
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