Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile

Fernández-Fernández, Rocío; Hernández, Sara B.; Puerta Fernández, Elena; Sánchez-Romero, María A.; Urdaneta, Veronica; Casadesús, Josep; Ministerio de Economía, Industria y Competitividad (España)

Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile

14 páginas.- 6 figuras.- 2 tablas.- referencias.- The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2021.647305/full#supplementary-material

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Main Authors:	Fernández-Fernández, Rocío, Hernández, Sara B., Puerta Fernández, Elena, Sánchez-Romero, María A., Urdaneta, Veronica, Casadesús, Josep
Other Authors:	Ministerio de Economía, Industria y Competitividad (España)
Format:	artículo biblioteca
Language:	English
Published:	Frontiers Media 2021-02-25
Subjects:	Salmonella, Bile resistance, Z-ring, Lon protease, MicA sRNA,
Online Access:	http://hdl.handle.net/10261/235697 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/100009042 http://dx.doi.org/10.13039/501100010198 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011011
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spelling	dig-irnas-es-10261-2356972021-12-27T16:40:46Z Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile Fernández-Fernández, Rocío Hernández, Sara B. Puerta Fernández, Elena Sánchez-Romero, María A. Urdaneta, Veronica Casadesús, Josep Ministerio de Economía, Industria y Competitividad (España) European Commission Agencia Estatal de Investigación (España) Junta de Andalucía Universidad de Sevilla Puerta Fernández, Elena [0000-0003-3559-6791] Sánchez-Romero, María A. [0000-0003-3098-1033] Casadesús, Josep [0000-0002-2308-293X] Salmonella Bile resistance Z-ring Lon protease MicA sRNA 14 páginas.- 6 figuras.- 2 tablas.- referencias.- The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2021.647305/full#supplementary-material Genes annotated as ygfE and yiiU in the genome of Salmonella enterica serovar Typhimurium encode proteins homologous to Escherichia coli cell division factors ZapA and ZapB, respectively. ZapA− and ZapB− mutants of S. enterica are bile-sensitive. The amount of zapB mRNA increases in the presence of a sublethal concentration of sodium deoxycholate (DOC) while zapA mRNA remains unaffected. Increased zapB mRNA level in the presence of DOC is not caused by upregulation of zapB transcription but by increased stability of zapB mRNA. This increase is suppressed by an hfq mutation, suggesting the involvement of a small regulatory RNA. We provide evidence that such sRNA is MicA. The ZapB protein is degraded in the presence of DOC, and degradation appears to involve the Lon protease. We propose that increased stability of zapB mRNA in the presence of DOC may counter degradation of bile-damaged ZapB, thereby providing sufficient level of functional ZapB protein to permit Z-ring assembly in the presence of bile This study was supported by grant BIO2016-75235-P from the Spanish Ministerio de Economía, Industria y Competitividad - Agencia Estatal de Investigación and the European Regional Fund. VU was supported by a postdoctoral contract associated to grant CVI-5879 from the Consejería de Innovación, Ciencia y Empresa, Junta de Andalucía, Spain. MS-R was supported by the VI Plan Propio de Investigación y Transferencia de la Universidad de Sevilla. Peer reviewed 2021-03-24T11:14:37Z 2021-03-24T11:14:37Z 2021-02-25 artículo http://purl.org/coar/resource_type/c_6501 Frontiers in Microbiology 12: 647305 (2021) http://hdl.handle.net/10261/235697 10.3389/fmicb.2021.647305 1664-302X http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/100009042 http://dx.doi.org/10.13039/501100010198 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011011 33717045 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-75235-P Publisher's version http://dx.doi.org/10.3389/fmicb.2021.647305 Sí open Frontiers Media
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collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-irnas-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del IRNAS España
language	English
topic	Salmonella Bile resistance Z-ring Lon protease MicA sRNA Salmonella Bile resistance Z-ring Lon protease MicA sRNA
spellingShingle	Salmonella Bile resistance Z-ring Lon protease MicA sRNA Salmonella Bile resistance Z-ring Lon protease MicA sRNA Fernández-Fernández, Rocío Hernández, Sara B. Puerta Fernández, Elena Sánchez-Romero, María A. Urdaneta, Veronica Casadesús, Josep Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile
description	14 páginas.- 6 figuras.- 2 tablas.- referencias.- The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2021.647305/full#supplementary-material
author2	Ministerio de Economía, Industria y Competitividad (España)
author_facet	Ministerio de Economía, Industria y Competitividad (España) Fernández-Fernández, Rocío Hernández, Sara B. Puerta Fernández, Elena Sánchez-Romero, María A. Urdaneta, Veronica Casadesús, Josep
format	artículo
topic_facet	Salmonella Bile resistance Z-ring Lon protease MicA sRNA
author	Fernández-Fernández, Rocío Hernández, Sara B. Puerta Fernández, Elena Sánchez-Romero, María A. Urdaneta, Veronica Casadesús, Josep
author_sort	Fernández-Fernández, Rocío
title	Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile
title_short	Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile
title_full	Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile
title_fullStr	Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile
title_full_unstemmed	Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile
title_sort	evidence for involvement of the salmonella enterica z-ring assembly factors zapa and zapb in resistance to bile
publisher	Frontiers Media
publishDate	2021-02-25
url	http://hdl.handle.net/10261/235697 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/100009042 http://dx.doi.org/10.13039/501100010198 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011011
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Evidence for Involvement of the Salmonella enterica Z-Ring Assembly Factors ZapA and ZapB in Resistance to Bile

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